Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Debra Weigl"'
Autor:
Byron Ellis, Debra Weigl, Jeff T. Hutchins, Mark de Serres, Randolph B. DePrince, John E. Dillberger, Sharon K. Rudolph, Christine M. Boytos
Publikováno v:
STEM CELLS. 17:203-209
Administration of exogenous proteins and peptides as therapeutics carries with it the potential for immune system recognition and the development of neutralizing antibodies to endogenous regulatory proteins. PEGylation of proteins typically reduces t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a9781d3c6ff73d43af74cc08030aac9
https://doi.org/10.1002/stem.170203
https://doi.org/10.1002/stem.170203
Autor:
Michael A. Luther, Brett Lovejoy, Anne Cleasby, Debra Weigl, Gerard McGeehan, Steven R. Jordan, Anne M. Hassell, Millard H. Lambert
Publikováno v:
Annals of the New York Academy of Sciences. 732:375-378
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60c68696de324d5fb8ecc5ce341bcee6
https://doi.org/10.1111/j.1749-6632.1994.tb24758.x
https://doi.org/10.1111/j.1749-6632.1994.tb24758.x
Publikováno v:
Biochemistry. 33:8207-8217
Collagenase is a member of the matrix metalloproteinase (MMP) family of enzymes. Aberrant regulation of this family has been implicated in pathologies such as arthritis and metastasis. Two crystal forms of the catalytic (19-kDa) domain of human fibro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1316164a29bc97d2ecb8cb9cc989ed26
https://doi.org/10.1021/bi00193a006
https://doi.org/10.1021/bi00193a006
Autor:
Kathryn S. Albrecht, Robert L. Simmer, William E. Kohlbrenner, Mary C. Turon, John W. Erickson, Debra Weigl, Federico Mordini, Judith Rittenhouse, Rosalind Helfrich
Publikováno v:
Biochemical and Biophysical Research Communications. 171:60-66
A procedure is described which employs pepstatin-agarose for the affinity purification of either HIV-1 or HIV-2 protease from two similar recombinant E. coli constructs that were developed for the expression of these enzymes. HIV-2 protease was routi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c2c1f8ac2c75c5070a7b9f37a5959b1
https://doi.org/10.1016/0006-291x(90)91356-w
https://doi.org/10.1016/0006-291x(90)91356-w
Autor:
Clara R. Smith, Debra Weigl, Magnus Persmark, Jennifer Dackor, Jack D. Griffith, Randolph B. DePrince
Publikováno v:
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 854(1-2)
We sought to establish a single anion-exchange HPLC method for the separation of linear, open circular and supercoiled plasmid topoisomers using purified topoisomeric forms of three plasmids (3.0, 5.5 and 7.6 kb). However, finding one condition prove
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::916585c978d9a472866714e4c9fa02c4
https://pubmed.ncbi.nlm.nih.gov/17481973
https://pubmed.ncbi.nlm.nih.gov/17481973
Autor:
Pierre Graber, William Burkhart, Michael A. Luther, Gardiner F.H. Smith, Steven R. Jordan, Michael V. Milburn, Timothy N. C. Wells, Anne M. Hassell, Debra Weigl, Robert J. Anderegg
Publikováno v:
Journal of molecular biology. 236(5)
Crystals of the catalytic domain of human fibroblast collagenase have been grown in the presence and absence of an inhibitor. Crystals of the inhibitor complex grew from 0.2 M ammonium sulfate and 15 to 30% PEG 8000 at 22 degrees C as bipyramids in t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d69cfd655110b647906994a045564395
https://pubmed.ncbi.nlm.nih.gov/8126730
https://pubmed.ncbi.nlm.nih.gov/8126730
Autor:
Andrew B. McElroy, Gerard McGeehan, Anne M. Hassell, Brett Lovejoy, David H. Drewry, Anne Cleasby, Steven R. Jordan, Kelly Longley, Michael A. Luther, Debra Weigl, Millard H. Lambert
Publikováno v:
Science (New York, N.Y.). 263(5145)
Collagenase is a zinc-dependent endoproteinase and is a member of the matrix metalloproteinase (MMP) family of enzymes. The MMPs participate in connective tissue remodeling events and aberrant regulation has been associated with several pathologies.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0788edfed161c8ff65507c32985a5de3
https://pubmed.ncbi.nlm.nih.gov/8278810
https://pubmed.ncbi.nlm.nih.gov/8278810
Autor:
David Brown, Alison Taylor, Mary Maus, Mary C. Turon, William E. Kohlbrenner, Sunil Kadam, Leonard Katz, Debra Weigl
Publikováno v:
Applied Microbiology and Biotechnology. 37
A 1.3-kb segment of Escherichia coli DNA containing the regulatory gene, araC, and the promoter of the araBAD operon was amplified by the polymerase chain reaction (PCR) and cloned into pUC18, resulting in plasmid pKB130 that produced the alpha fragm
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::529d843e1465673bf23acdb0c3f847b2
https://doi.org/10.1007/bf00178172
https://doi.org/10.1007/bf00178172
Publikováno v:
Biochemical and Biophysical Research Communications. 159:842-847
A heteronuclear three-dimensional NMR experiment has been applied to uniformly 15N-labeled CMP-KDO synthetase (CTP:3-deoxy-D-manno-octulosonate cytidylyl transferase; E.C. 2.7.7.38) complexed with an inhibitor and CTP. Using this 3D technique, the 2D
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e7a63ef968029bbc69c5098b7a64ff5
https://doi.org/10.1016/0006-291x(89)90071-5
https://doi.org/10.1016/0006-291x(89)90071-5