Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Deborah Y. Kwoh"'
Autor:
Mariusz G. Banaszczyk, Charles P. Lollo, Patricia M. Mullen, Steven W. Brostoff, Alison T. Phillips, Arjang Amini, Christopher C. Coffin, Duncan P. Wu, Deborah Y. Kwoh, Dennis J. Carlo
Publikováno v:
Journal of Macromolecular Science, Part A. 36:1061-1084
Polycations have been used for gene delivery in vitro quite successfully, however, in vivo applications suffered from serum effects that lower the overall gene drug efficiency. PEG polymers have been used extensively to minimize serum effects and cre
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::00c59ba433b2b6a965a17392d4679882
https://doi.org/10.1080/10601329908951200
https://doi.org/10.1080/10601329908951200
Publikováno v:
Veterinary Microbiology. 24:235-251
The capability to detect the genetic elements (DNA or RNA) of a particular pathogen as a means of identifying the infectious agent has been the traditional function of nucleic acid hybridization assays. The low copy number of genetic material from se
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc8520df3745f3e8c1564fa70eb11813
https://doi.org/10.1016/0378-1135(90)90174-t
https://doi.org/10.1016/0378-1135(90)90174-t
Publikováno v:
An Introduction to Molecular Medicine and Gene Therapy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::01f714e9d524ceffd6ec178a36fc759c
https://doi.org/10.1002/0471223875.app
https://doi.org/10.1002/0471223875.app
Publikováno v:
Biochimica et biophysica acta. 1467(1)
Phospholipid (PL) scramblase is a 35 kDa protein that is thought to mediate Ca2+-induced bidirectional transbilayer movement of plasma membrane phospholipids in activated, injured, or apoptotic cells. We recently reported the molecular cloning of a P
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54574ddc217d3a921f074ed5d7249d65
https://pubmed.ncbi.nlm.nih.gov/10930526
https://pubmed.ncbi.nlm.nih.gov/10930526
Autor:
Richard M. Bartholomew, Alison T. Phillips, Christopher C. Coffin, Charles P. Lollo, Steven W. Brostoff, Mariusz G. Banaszczyk, Dennis J. Carlo, Jocelyn Jovenal, Patricia M. Mullen, Deborah Y. Kwoh, Arjang Amini, Joanne M Fabrycki
Publikováno v:
Biochimica et biophysica acta. 1444(2)
We are developing a self-assembling non-viral in vivo gene delivery vehicle based on poly-l-lysine and plasmid DNA. We have characterized poly-l-lysines of different chain lengths for DNA condensation and strength of DNA binding. Poly-l-lysine chains
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bfcb161793fe1f9e1b38aaf237174e01
https://pubmed.ncbi.nlm.nih.gov/10023051
https://pubmed.ncbi.nlm.nih.gov/10023051
Autor:
Thomas R. Gingeras, Deborah Y. Kwoh
Publikováno v:
Applied Virology Research ISBN: 9781475792676
The advances in nucleic acid sequencing and detection by hybridization have opened new approaches to the diagnosis of human pathogens. While nucleic acid hybridization made detection of target sequences highly specific and/or relatively rapid in comp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fba896b84d21752a3984c6c87f976eed
https://doi.org/10.1007/978-1-4757-9265-2_3
https://doi.org/10.1007/978-1-4757-9265-2_3
Publikováno v:
Canadian journal of microbiology. 39(4)
Polymerase chain reaction amplification was used to compare different regions of the cytochrome c3 gene from nine strains of Desulfovibrio vulgaris, to examine homology within the species. Six 30-base polymerase chain reaction primers and three probe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa79ebcc93618a80c3855f2f732c9294
https://pubmed.ncbi.nlm.nih.gov/8388770
https://pubmed.ncbi.nlm.nih.gov/8388770
Autor:
John C. Guatelli, Deborah Y. Kwoh, Kevin J. Barringer, Kristina Marie Whitfield, Thomas R. Gingeras, Douglas D. Richman
Publikováno v:
Scopus-Elsevier
A target nucleic acid sequence can be replicated (amplified) exponentially in vitro under isothermal conditions by using three enzymatic activities essential to retroviral replication: reverse transcriptase, RNase H, and a DNA-dependent RNA polymeras
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25379fe1b0d3d3d8d08d070fdfea5a96
https://doi.org/10.1073/pnas.87.19.7797-a
https://doi.org/10.1073/pnas.87.19.7797-a
Publikováno v:
Analytical Biochemistry. 178:43-51
A hydrazone-based method for conjugating synthetic nucleic acids and reporter molecules for use as nonradioactive hybridization probes is presented. Oligonucleotides complementary to the hepatitis B virus were derivatized at their 5′ ends with hydr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee5be473a7d70d8304105e7c7017634e
https://doi.org/10.1016/0003-2697(89)90354-0
https://doi.org/10.1016/0003-2697(89)90354-0
Autor:
Thomas R. Gingeras, G. R. Davis, Kristina Marie Whitfield, H. L. Chappelle, Deborah Y. Kwoh, L. J. DiMichele
Publikováno v:
Scopus-Elsevier
The in vitro amplification of biologically important nucleic acids has proceeded principally by a strategy of DNA replication. Polymerase chain reaction was the first such protocol to achieve this goal. In this report, a transcription-based amplifica
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a64ad1aaf472da64d7410f13927a556
https://doi.org/10.1073/pnas.86.4.1173
https://doi.org/10.1073/pnas.86.4.1173