The low information content of Neurospora splicing signals: implications for RNA splicing and intron origin

Autor: Deborah J. Field, Jason E. Stajich, Diane M. DeAbreu, Joan E. Olive, Richard A. Collins

Publikováno v: Collins, RA; Stajich, JE; Field, DJ; Olive, JE; & Deabreu, DM. (2015). The low information content of Neurospora splicing signals: Implications for RNA splicing and intron origin. RNA, 21(5), 997-1004. doi: 10.1261/rna.047985.114. UC Riverside: Retrieved from: http://www.escholarship.org/uc/item/8n51c387

When we expressed a small (0.9 kb) nonprotein-coding transcript derived from the mitochondrial VS plasmid in the nucleus of Neurospora we found that it was efficiently spliced at one or more of eight 5′ splice sites and ten 3′ splice sites, which

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::318dcc2351b5470729069d86b3b3e8f9
https://doi.org/10.1261/rna.047985.114

Synthetic Lethality of Yeast slt Mutations with U2 Small Nuclear RNA Mutations Suggests Functional Interactions between U2 and U5 snRNPs That Are Important for Both Steps of Pre-mRNA Splicing

Autor: Deborah J. Field, Arnaud Moris, Brian P. Bobechko, Deming Xu, James D. Friesen, Shou-Jiang Tang

Publikováno v: Molecular and Cellular Biology. 18:2055-2066

A genetic screen was devised to identify Saccharomyces cerevisiae splicing factors that are important for the function of the 5' end of U2 snRNA. Six slt (stands for synthetic lethality with U2) mutants were isolated on the basis of synthetic lethali

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0284634a891b8d867b023bd951a0bea9
https://doi.org/10.1128/mcb.18.4.2055

The Wiskott-Aldrich syndrome protein acts downstream of CD2 and the CD2AP and PSTPIP1 adaptors to promote formation of the immunological synapse

Autor: Lynne A. Hardy, Fabio Shi, Karen Badour, Deborah J. Field, Mary K. H. McGavin, Katherine A. Siminovitch, Jinyi Zhang, Vik Rampersad

Publikováno v: Immunity. 18(1)

The Wiskott-Aldrich syndrome protein (WASp) couples actin cytoskeletal rearrangement to T cell activation, but the mechanisms involved are unknown. Here, we show that antigen-induced formation of T cell:APC conjugates and synapses is abrogated in WAS

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bfb32b873a7fa674630580ced49a5c84
https://pubmed.ncbi.nlm.nih.gov/12530983

Polymorphism of brain tubulin

Autor: Deborah J. Field, James C. Lee, Lily Misra, Henry J. George

Publikováno v: Biochemistry. 20:2402-2409

Calf brain tubulin was subjected to isoelectric focusing and tryptic peptide map analysis. Results from isoelectric focusing experiments showed a total number of 17 well-resolved protein peaks. The number of peaks and the mass distribution under each

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::926205db51c4098cd8952570a2bfe412
https://doi.org/10.1021/bi00512a006

Effects of nocodazole on structures of calf brain tubulin

Autor: Lucy L. Y. Lee, James Lee, Deborah J. Field

Publikováno v: Biochemistry. 19:6209-6215

The interaction of nocodazole with calf brain tubulin was studied to determine the effect of such interaction on the structure of tubulin. The effect of nocodazole on the self-association of tubulin was monitored by turbidity measurements and velocit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cfcbc665c244da2ee787b8a5c52d1400
https://doi.org/10.1021/bi00567a041