Engineering of Escherichia coli for Krebs cycle-dependent production of malic acid

Autor: Debora Trichez, Clément Auriol, Audrey Baylac, Romain Irague, Clémentine Dressaire, Marc Carnicer-Heras, Stéphanie Heux, Jean Marie François, Thomas Walther

Publikováno v: Microbial Cell Factories, Vol 17, Iss 1, Pp 1-12 (2018)

Abstract Background Malate is a C4-dicarboxylic acid widely used as an acidulant in the food and beverage industry. Rational engineering has been performed in the past for the development of microbial strains capable of efficient production of this m

Externí odkaz: https://doaj.org/article/a3de523a6c104bd59c4a1d068bd06ebb

Cell Surface Phenotypic Diversity and Flocculation Gene Variability in Contaminant Industrial Fuel-Ethanol Yeast Strains Exhibiting Highly Foaming Phenotypes

Autor: Henrique Amorim, Boris U. Stambuk, Debora Trichez, Daniella H Hock, M. L. Lopes, Maria de Lurdes B. Magalhães, Catarina M. de Figueiredo

Many contaminant yeast strains able to survive inside fuel ethanol industrial vats show detrimental cell surface phenotypes, such as filamentation, invasive growth, flocculation, biofilm formation and excessive foam production. Previous studies have

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87affb0fd3e002a28e0f480b3d68e793
https://doi.org/10.20944/preprints202106.0719.v1

Applications of the Methylotrophic Yeast Komagataella phaffii in the Context of Modern Biotechnology

Autor: Lidia Maria Pepe de Moraes, Henrique Fetzner Marques, Viviane Castelo Branco Reis, Cintia Marques Coelho, Matheus de Castro Leitão, Alexsandro Sobreira Galdino, Thais Paiva Porto de Souza, Luiza Cesca Piva, Ana Laura Alfonso Perez, Débora Trichez, João Ricardo Moreira de Almeida, Janice Lisboa De Marco, Fernando Araripe Gonçalves Torres

Publikováno v: Journal of Fungi, Vol 10, Iss 6, p 411 (2024)

Komagataella phaffii (formerly Pichia pastoris) is a methylotrophic yeast widely used in laboratories around the world to produce recombinant proteins. Given its advantageous features, it has also gained much interest in the context of modern biotech

Externí odkaz: https://doaj.org/article/e49d134356bf46a598f964e553688b7f

The synthetic xylulose-1 phosphate pathway increases production of glycolic acid from xylose-rich sugar mixtures

Autor: Jean Marie François, Thomas Walther, Lucie Spina, Ceren Alkim, Debora Trichez, Yvan Cam

Publikováno v: Biotechnology for Biofuels
Biotechnology for Biofuels, BioMed Central, 2016, 9 (1), ⟨10.1186/s13068-016-0610-2⟩
Biotechnology for Biofuels (9), . (2016)
Biotechnology for Biofuels, 2016, 9 (1), ⟨10.1186/s13068-016-0610-2⟩

Background: Glycolic acid (GA) is a two-carbon hydroxyacid with applications in the cosmetic, textile, and medical industry. Microbial GA production from all sugars can be achieved by engineering the natural glyoxylate shunt. The synthetic (D)-xylulo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85b91a9c879401b68adce866701e8456
https://hal.archives-ouvertes.fr/hal-01886414/document

Engineering of a Synthetic Metabolic Pathway for the Assimilation of (d)-Xylose into Value-Added Chemicals

Autor: Debora Trichez, François Bartolo, Vincent Trebosc, Jean Marie François, Amélie Vax, Philippe Besse, Ceren Alkim, Thomas Walther, Yvan Cam

Publikováno v: ACS Synthetic Biology
ACS Synthetic Biology, American Chemical Society, 2016, 5 (7), pp.607-618. ⟨10.1021/acssynbio.5b00103⟩
ACS Synthetic Biology, 2016, 5 (7), pp.607-618. ⟨10.1021/acssynbio.5b00103⟩

A synthetic pathway for (D)-xylose assimilation was stoichiometrically evaluated and implemented in Escherichia coli strains. The pathway proceeds via isomerization of (D)-xylose to (D)-xylulose, phosphorylation of (D)-xylulose to obtain (D)-xylulose

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::349e7f772e1772ee502c12596f6937b3
https://pubmed.ncbi.nlm.nih.gov/26186096