Zobrazeno 1 - 10
of 16
pro vyhledávání: '"De-Xiang Gu"'
Publikováno v:
Enzyme and Microbial Technology. 24:647-650
Streptokinase (SK) is a specific effective medicine for thrombolytic therapy of acute myocardial infarction. This study established a process for the pilot production of recombinant streptokinase (r-SK). Engineering bacteria were fermented in a 20-l
Publikováno v:
Process Biochemistry. 34:55-58
High cell density fermentation production of recombinant human granulocyte-macrophage colony stimulating factor (GM-CSF) was studied in an Escherichia coli secretory expression system. By using glycerol instead of glucose as a carbon source and feedi
Publikováno v:
Process Biochemistry. 33:683-686
High cell density fermentation production of recombinant human growth hormone was studied in Escherichia coli. The results showed that by using glycerol instead of glucose as carbon source and feeding in a specific fed-batch mode, by controlling the
Publikováno v:
Journal of Food Engineering. 37:103-110
Supercritical carbon dioxide was employed to extract wheat plumule oil in this study. Effects of pressure, temperature, CO 2 solvent flows and extraction time on the extraction efficiency were investigated. Three models were set up to describe extrac
Publikováno v:
Enzyme and Microbial Technology. 22:205-209
A group of time-dependent kinetic models for glutamic acid fermentation are presented. These can divide cell growth into three stages: the positive acceleration stage, negative acceleration stage, and equilibrium stage. The substrate consumption is a
Publikováno v:
Enzyme and Microbial Technology. 21:165-169
It is different from traditional considerations that the concentrations of cell growth (X), substrate consumption (S), and product formation (P) during a fermentation process are put into a three-dimensional space (X, P, S). It is found that the coor
Publikováno v:
Journal of the Science of Food and Agriculture. 70:303-306
The desorption isotherms of potato, carrot, tomato and green pepper were determined at 30, 40, 50 and 60°C and within the range of 10-90% water activity. Iglesias and Chirife, Halsey and GAB equations were tested to fit the experimental data. The ne
Publikováno v:
Bioresource Technology. 70:299-301
A cDNA fragment coding human tumor necrosis factor-alpha (TNF-α) was inserted into the vector pSXIVVI + X3 with the control of Syn XIV promoter. The Sf9 cells ( Spodoptera frugiperda ) were co-transfected with the recombinant plasmid and TnNPV DNA (
Publikováno v:
Bioresource Technology. 68:201-203
A cDNA fragment coding human interleukin-6 (IL-6) was inserted into the vector pSXIVVI + X3 with the control of Syn and XIV promoters. The Sf9 cells ( Spodoptera frugiperda ) were co-transfected with the recombinant plasmid and TnNPV DNA ( Trichoplus
Publikováno v:
Journal of Industrial Microbiology and Biotechnology. 21:175-177
Using pSXIVVI + X3 as an expressing vector, an occluded recombinant Trichoplusia ni nuclear polyhedrosis virus carrying the cDNA encoding plasminogen activators inhibitor-2 (PAI-2) under the control of the Syn and XIV promoters, has been constructed.