Zobrazeno 1 - 10
of 81
pro vyhledávání: '"David W. Emery"'
Autor:
Monica Volpin, Georgios Kaltsounis, Minas Yiangou, Eugenio Montini, Nikoletta Psatha, Mingdong Liu, Suming Huang, Evangelia Yannaki, David W. Emery, George Stamatoyannopoulos, Thalia Papayannopoulou, Penelope-Georgia Papayanni, Xing-Guo Li, Panayota Christofi, Achilles Anagnostopoulos, Pamela Melo
Publikováno v:
Hum Gene Ther
Despite the unequivocal success of hematopoietic stem and progenitor cell gene therapy, limitations still exist including genotoxicity and variegation/silencing of transgene expression. A class of DNA regulatory elements known as chromatin insulators
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::36f1d2ebdb416dd221ffcd7c7041517b
https://doi.org/10.1089/hum.2021.142
https://doi.org/10.1089/hum.2021.142
Publikováno v:
PLoS ONE, Vol 10, Iss 4, p e0124588 (2015)
Sequences of the genome that are capable of silencing gene expression are thought to play a key role in gene regulation. However, very few silencer elements capable of functioning in mammalian cells have been described, and only a fraction of these h
Externí odkaz:
https://doaj.org/article/74aef74c0f884cefbe0a251cb4107cb4
Publikováno v:
BioTechniques, Vol 39, Iss 4, Pp 553-557 (2005)
The bacteriophage λ recombination system is increasingly used for recombinant DNA applications that involve the frequent transfer of sequences into and between shuttle and reporter vectors. This approach bypasses the need for restriction endonucleas
Externí odkaz:
https://doaj.org/article/ecce0e9095494e48b21ea628d05ddd9a
Publikováno v:
PLoS ONE, Vol 8, Iss 10, p e76528 (2013)
The chromatin insulator cHS4 can reduce silencing chromosomal position effects and genotoxicity associated with integrating viral vectors. However, the fully active version of this element can also reduce vector titers and is only partially effective
Externí odkaz:
https://doaj.org/article/0fcbb5f656c34c0da5d42b5cec8193f9
Autor:
Arnold J. Levine, Hans-Peter Kiem, Dale Ando, David W. Emery, Tony R. Reid, Christopher L. Jenkins, Carl H. June, Mark L. Bagarazzi
Publikováno v:
Molecular Therapy. 24:1173-1177
The year 2015 marked the 40th anniversary of the Asilomar Conference leading to the first biosafety guidelines for research with recombinant DNA molecules,1 as well as the 25th anniversary of the first human gene transfer clinical trial approved by t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9103b2f46bd5b000d0bd89a857c85710
https://doi.org/10.1038/mt.2016.133
https://doi.org/10.1038/mt.2016.133
Autor:
Mingdong Liu, Chao Zhong Song, Patrick A. Navas, David W. Emery, George Stamatoyannopoulos, Hao Wang, Matthew T. Maurano, John A. Stamatoyannopoulos, Heyuan Qi
Publikováno v:
Nature Biotechnology. 33:198-203
Insertional mutagenesis and genotoxicity, which usually manifest as hematopoietic malignancy, represent major barriers to realizing the promise of gene therapy. Although insulator sequences that block transcriptional enhancers could mitigate or elimi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb3ee893807db1dcc74fb2a2dd7ad643
https://doi.org/10.1038/nbt.3062
https://doi.org/10.1038/nbt.3062
Publikováno v:
Cytogenetic and genome research. 151(2)
Integrating gammaretroviral vectors can dysregulate the expression of cellular genes through a variety of mechanisms, leading to genotoxicity and malignant transformation. Although most attention has focused on the activation of cellular genes by vec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4671a63bd50ff32761af62ca21f4c604
https://pubmed.ncbi.nlm.nih.gov/28433994
https://pubmed.ncbi.nlm.nih.gov/28433994
Autor:
David W. Emery
Publikováno v:
Human Gene Therapy. 22:761-774
The therapeutic application of recombinant retroviruses and other integrating gene transfer vectors has been limited by problems of vector expression and vector-mediated genotoxicity. These problems arise in large part from the interactions between v
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a3f353408069e3c2a73bc04f91a0eef
https://doi.org/10.1089/hum.2010.233
https://doi.org/10.1089/hum.2010.233
Publikováno v:
Journal of Biological Chemistry. 285:37589-37597
The prototypic chromatin insulator cHS4 has proven effective in reducing silencing chromosomal position effects in a variety of settings. Most of this barrier insulator activity has been mapped to a 250-bp core region, as well as to several proteins
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e37b19eea66ba1357a1398c6119cb965
https://doi.org/10.1074/jbc.m110.174532
https://doi.org/10.1074/jbc.m110.174532
Publikováno v:
Molecular Therapy. 17(4):716-724
Interest in the use of recombinant retroviral vectors for clinical gene therapy has been tempered by evidence of vector-mediated genotoxicity involving the activation of cellular oncogenes flanking sites of vector integration. We report here that the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a06f6da7b99e192e705257631f90e354