The tunable pReX expression vector enables optimizing the T7-based production of membrane and secretory proteins in E. coli

Autor: Grietje Kuipers, Alexandros Karyolaimos, Zhe Zhang, Nurzian Ismail, Gianluca Trinco, David Vikström, Dirk Jan Slotboom, Jan-Willem de Gier

Publikováno v: Microbial Cell Factories, Vol 16, Iss 1, Pp 1-11 (2017)

Abstract Background To optimize the production of membrane and secretory proteins in Escherichia coli, it is critical to harmonize the expression rates of the genes encoding these proteins with the capacity of their biogenesis machineries. Therefore,

Externí odkaz: https://doaj.org/article/5461ebc22f7a43058e93cdf812a4c890

Mutagenesis-Based Characterization and Improvement of a Novel Inclusion Body Tag

Autor: Wouter S. P. Jong, Corinne M. ten Hagen-Jongman, David Vikström, Wendy Dontje, Abdallah M. Abdallah, Jan-Willem de Gier, Wilbert Bitter, Joen Luirink

Publikováno v: Frontiers in Bioengineering and Biotechnology, 7. Frontiers Research Foundation
Frontiers in Bioengineering and Biotechnology, Vol 7 (2020)
Jong, W S P, ten Hagen-Jongman, C M, Vikström, D, Dontje, W, Abdallah, A M, de Gier, J W, Bitter, W & Luirink, J 2020, ' Mutagenesis-Based Characterization and Improvement of a Novel Inclusion Body Tag ', Frontiers in Bioengineering and Biotechnology, vol. 7, 442 . https://doi.org/10.3389/fbioe.2019.00442
Frontiers in Bioengineering and Biotechnology, 7:442. Frontiers Research Foundation
Frontiers in Bioengineering and Biotechnology
Jong, W S P, Ten Hagen-Jongman, C M, Vikström, D, Dontje, W, Abdallah, A M, de Gier, J-W, Bitter, W & Luirink, J 2019, ' Mutagenesis-Based Characterization and Improvement of a Novel Inclusion Body Tag ', Frontiers in Bioengineering and Biotechnology, vol. 7, pp. 442 . https://doi.org/10.3389/fbioe.2019.00442

Whereas, bacterial inclusion bodies (IBs) for long were regarded as undesirable aggregates emerging during recombinant protein production, they currently receive attention as promising nanoparticulate biomaterials with diverse applications in biotech

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::043bc97aa4f9da7cc897cca2e9b08b31
https://research.vumc.nl/en/publications/81555503-6216-45c2-9b8a-4e851cc1ed04

Autotransporter-based antigen display in bacterial ghosts

Autor: Joen Luirink, Bill Söderström, Jan-Willem de Gier, Anna Hjelm, Wouter S. P. Jong, David Vikström

Publikováno v: Applied and Environmental Microbiology, 81(2), 726-735. American Society for Microbiology
Hjelm, A, Söderström, B, Vikström, D, Jong, W S P, Luirink, S & de Gier, J W 2015, ' Autotransporter-based antigen display in bacterial ghosts. ', Applied and Environmental Microbiology, vol. 81, no. 2, pp. 726-735 . https://doi.org/10.1128/AEM.02733-14

Bacterial ghosts are empty cell envelopes of Gram-negative bacteria that can be used as vehicles for antigen delivery. Ghosts are generated by releasing the bacterial cytoplasmic contents through a channel in the cell envelope that is created by the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fcd1fe18402fe4372aa2911f56f7e131
https://research.vu.nl/en/publications/cef38071-2faa-4159-bfc5-bb3446ebd878

Application of an E. coli signal sequence as a versatile inclusion body tag

Autor: Jan-Willem de Gier, Joen Luirink, Diane Houben, Wouter S. P. Jong, H. Bart van den Berg van Saparoea, David Vikström

Publikováno v: Microbial Cell Factories, 16(1):50. BioMed Central
Microbial Cell Factories
Jong, W S P, Vikström, D, Houben, D, Berg van Saparoea, H B, de Gier, J W & Luirink, J 2017, ' Application of an E. coli signal sequence as a versatile inclusion body tag ', Microbial Cell Factories, vol. 16, no. 1, 50 . https://doi.org/10.1186/s12934-017-0662-4

Background Heterologous protein production in Escherichia coli often suffers from bottlenecks such as proteolytic degradation, complex purification procedures and toxicity towards the expression host. Production of proteins in an insoluble form in in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d5686686223213905d6d327b222e52a
https://doi.org/10.1186/s12934-017-0662-4

Bacterial-based membrane protein production

Autor: Jan-Willem de Gier, David Vikström, Anna Hjelm, Thomas Baumgarten, Susan Schlegel

Publikováno v: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1843(8):1739-1749

Escherichia coli is by far the most widely used bacterial host for the production of membrane proteins. Usually, different strains, culture conditions and production regimes are screened for to design the optimal production process. However, these E.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::829212a36ea5e08e22216abae3176d32