Non-random spatial organization of telomeres varies during the cell cycle and requires LAP2 and BAF

Autor: Debora Keller, Sonia Stinus, David Umlauf, Edith Gourbeyre, Eric Biot, Nicolas Olivier, Pierre Mahou, Emmanuel Beaurepaire, Philippe Andrey, Laure Crabbe

Publikováno v: iScience, Vol 27, Iss 4, Pp 109343- (2024)

Summary: Spatial genome organization within the nucleus influences major biological processes and is impacted by the configuration of linear chromosomes. Here, we applied 3D spatial statistics and modeling on high-resolution telomere and centromere 3

Externí odkaz: https://doaj.org/article/56dab50fd97b44fab26e59dcc95207fe

UVB induces a genome-wide acting negative regulatory mechanism that operates at the level of transcription initiation in human cells.

Autor: Akos Gyenis, David Umlauf, Zsuzsanna Ujfaludi, Imre Boros, Tao Ye, Làszlò Tora

Publikováno v: PLoS Genetics, Vol 10, Iss 7, p e1004483 (2014)

Faithful transcription of DNA is constantly threatened by different endogenous and environmental genotoxic effects. Transcription coupled repair (TCR) has been described to stop transcription and quickly remove DNA lesions from the transcribed strand

Externí odkaz: https://doaj.org/article/391002d91f4243c39e17f890772d5747

Spatial modeling of telomere intra-nuclear distribution reveals non-random organization that varies during cell cycle and depends on LAP2 and BAF

Autor: Debora Keller, Sonia Stinus, David Umlauf, Edith Gourbeyre, Eric Biot, Nicolas Olivier, Pierre Mahou, Emmanuel Beaurepaire, Philippe Andrey, Laure Crabbe

Genome organization within the 3D nuclear volume influences major biological processes but is completely lost during mitosis, which represents a major challenge to maintain cellular identity and cell fate. To restore a functional G1 nucleus for the n

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6dd9e048cf69af9628b96b1ef08f126e
https://doi.org/10.1101/2022.12.22.521599

Methyl Adenine Identification (MadID): High-Resolution Detection of Protein-DNA Interactions

Autor: Michal Sobecki, Laure Crabbe, David Umlauf

Publikováno v: The nucleus
The nucleus, 2175, pp.123-138, 2020, Methods in Molecular Biology book series, ⟨10.1007/978-1-0716-0763-3_10⟩
The Nucleus ISBN: 9781071607626

Mapping the binding sites of DNA- or chromatin-interacting proteins is essential to understand many essential biological processes. Methyl Adenine Identification (MadID) is a proximity methylation-based assay that allows the visualization, quantifica

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ceeabba31d2a3c65f2d5cba9c06ecaf
https://hal.archives-ouvertes.fr/hal-03014022

Le génome intime… et en trois dimensions

Autor: David Umlauf

Publikováno v: médecine/sciences. 31:304-311

Au cours de la dernière décennie, les différentes techniques dites de capture de la conformation des chromosomes (3C) ont accéléré notre compréhension de l’architecture nucléaire des cellules eucaryotes. Couplées aux technologies de séque

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1c9b0459216497014aa9f4e37f490b9e
https://doi.org/10.1051/medsci/20153103016