Zobrazeno 1 - 10
of 119
pro vyhledávání: '"David S. Sigman"'
Publikováno v:
ChemBioChem. 2:735-740
Publikováno v:
Methods. 23:160-168
We have demonstrated that oligoribonucleotides that lack a 3'-OH group and cannot be extended by RNA polymerase can hybridize to the single-stranded DNA formed inside the transcription initiation bubble (or open complex) and inhibit transcription. Us
Publikováno v:
Proceedings of the National Academy of Sciences. 97:3136-3141
The single-stranded region of DNA within the open complex of transcriptionally active genes provides a unique target for the design of gene-specific transcription inhibitors. Using the Escherichia coli lac UV5 and trp EDCBA promoters as in vitro mode
Autor:
David S. Sigman, Thomas C. Terwilliger, Avram D. Walts, Ralf Landgraf, Chi Hong B. Chen, Lisa Chan, Petra M. Schlonk
Publikováno v:
Chemistry & Biology. 5(5):283-292
Background: We were interested in developing a protocol for cleaving large DNAs specifically. Previous attempts to develop such methods have failed to work because of high levels of nonspecific background scission. Results: R-loop formation was chose
Publikováno v:
Chemistry & Biology. 3(9):739-746
Background : The open complex formed at the initiation site of transcription within the active site of RNA polymerase is unique to actively transcribing genes and is thus an ideal target for the design of transcription inhibitors. Many redoxactive te
Publikováno v:
Nucleic Acids Research. 24:3246-3252
We have used R-loop formation and direct hybridization techniques to analyze the kinetics by which RNA is displaced from a heteroduplex by DNA of identical sequence. Using random walk simulations we were able to calculate the step times for a single
Autor:
Chi-hong B. Chen, Jamey L. Anderson, Christopher S. Foote, Yi-Zhong An, Yves Rubin, David S. Sigman
Publikováno v:
Tetrahedron. 52:5179-5189
A C60-linked deoxyoligonucleotide (C60-DON-1) was prepared from bromoacetate 3. This C60-oligonucleotide conjugate was hybridized to a complementary single-stranded DNA. This system reacted with light and oxygen to damage only guanosines in the singl
Autor:
David S. Sigman, Chi-hong B. Chen, Clark Pan, Young-Moon Cho, David M. Perrin, James Gallagher
Publikováno v:
Bioconjugate Chemistry. 7:413-420
Our interest in improving the efficiency of targeted scission reagents has prompted us to study the influence of ring substituents on the nuclease activity of 1,10-phenanthroline-copper conjugated to oligonucleotides and DNA-binding proteins. Since m
Publikováno v:
Nucleic Acids Research. 23:3524-3530
R-loop formation with short (100 nt) RNAs provides a highly flexible and stringent method to achieve sequence-specific separation of target DNA at any given sequence. After stabilization of R-loops with glyoxal and removal of the RNA through RNase tr
Publikováno v:
Nucleic Acids Research. 23:3516-3523
The sequence-specific formation of R-loops can be assayed using RNAs which overlap a HindIII cleavage site in a 3.5 kb plasmid. Chemical modification of the displaced DNA strand has permitted stabilization of these R-loops and allowed a systematic in