Zobrazeno 1 - 10
of 51
pro vyhledávání: '"David Rickwood"'
Publikováno v:
BioTechniques, Vol 37, Iss 2, Pp 276-281 (2004)
Previous work from this laboratory has shown that immunoporation has the potential for the selective transfection of a range of different animal cells based on their immunological identity. The unique ability of immunoporation to target cells for tra
Externí odkaz:
https://doaj.org/article/37c3a7524a1f4f858e1d5551e90a368e
Techniques for separating cells are needed in many areas of cell biology. This book presents modern methods from the laboratories of experts in the field, and includes tested, reproducible protocols, hints and tips for success, and troubleshooting su
Autor:
Carlos P. Rubbi, David Rickwood
Publikováno v:
Journal of Immunological Methods. 192:157-164
Activated T cells can release lymphokines selectively towards the site of contact with the target cell. In this way the specificity of the target-effector cell interaction can be maintained in spite of signalling being mediated by soluble factors (Mo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e55074ec9d85b1417eac27dda2e9ee3
https://doi.org/10.1016/0022-1759(96)00046-4
https://doi.org/10.1016/0022-1759(96)00046-4
Autor:
T.C. Ford, David Rickwood
Publikováno v:
Clinica Chimica Acta. 206:249-252
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3b553ba7aba96d4ab92103ee2d5d3973
https://doi.org/10.1016/0009-8981(92)90096-9
https://doi.org/10.1016/0009-8981(92)90096-9
Publikováno v:
Analytical Biochemistry. 187:318-323
Commonly, subcellular organelles such as nuclei, mitochondria, lysosomes, and Golgi membranes are isolated first by differential centrifugation in low-speed or high-speed centrifuges and then purified by gradient centrifugation in ultracentrifuges. W
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::964c775d02943dd083089024215a82bb
https://doi.org/10.1016/0003-2697(90)90463-j
https://doi.org/10.1016/0003-2697(90)90463-j
Autor:
David Rickwood
Publikováno v:
Cell Biology Protocols
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::574e6d2f1823fb99d5cf89fee2235c57
https://doi.org/10.1002/0470033487.ch7
https://doi.org/10.1002/0470033487.ch7
Publikováno v:
Cell Biology Protocols: Harris/Cell Biology Protocols
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::13bafd3515e8546e5a6c9d3841ffb080
https://doi.org/10.1002/0470033487
https://doi.org/10.1002/0470033487
Publikováno v:
Journal of immunoassayimmunochemistry. 26(3)
Immunoporation has been found to be able to efficiently transfect a wide range of human cultured cell lines. This report shows that peripheral blood mononuclear cells can also be efficiently transfected using immunoporation. The immunoporation of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e87fe20abe8ebef9b5f8c7d401daf28e
https://pubmed.ncbi.nlm.nih.gov/16011143
https://pubmed.ncbi.nlm.nih.gov/16011143
Autor:
David Rickwood, J. M. Graham
Publikováno v:
eLS
Mononuclear cells (lymphocytes and monocytes) can be isolated from other blood cell elements on the basis of their lower density. There are three variations of the basic method: (1) sedimentation on to a density barrier using diatrizoate (plus a poly
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7fd9ce6b4a5bd0006e6c34ade3c766bf
https://doi.org/10.1038/npg.els.0003801
https://doi.org/10.1038/npg.els.0003801
Autor:
David Rickwood, L Bildirici
Publikováno v:
Journal of immunological methods. 252(1-2)
This study has investigated possible alternative types of beads for fractionating cells on the basis of density perturbation. It is well known that uniform magnetic beads can be extremely important tools for separating cells by both magnetic separati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d88734d7328a0069365c0db724faf19e
https://pubmed.ncbi.nlm.nih.gov/11334965
https://pubmed.ncbi.nlm.nih.gov/11334965