Distribution of Holliday junctions and repair forks during Escherichia coli DNA double-strand break repair.

Autor: Tahirah Yasmin, Benura Azeroglu, Charlotte A Cockram, David R F Leach

Publikováno v: PLoS Genetics, Vol 17, Iss 8, p e1009717 (2021)

Accurate repair of DNA double-strand breaks (DSBs) is crucial for cell survival and genome integrity. In Escherichia coli, DSBs are repaired by homologous recombination (HR), using an undamaged sister chromosome as template. The DNA intermediates of

Externí odkaz: https://doaj.org/article/699560fd8ed641c9a2cef26281450fc3

Broken replication forks trigger heritable DNA breaks in the terminus of a circular chromosome.

Autor: Anurag Kumar Sinha, Christophe Possoz, Adeline Durand, Jean-Michel Desfontaines, François-Xavier Barre, David R F Leach, Bénédicte Michel

Publikováno v: PLoS Genetics, Vol 14, Iss 3, p e1007256 (2018)

It was recently reported that the recBC mutants of Escherichia coli, deficient for DNA double-strand break (DSB) repair, have a decreased copy number of their terminus region. We previously showed that this deficit resulted from DNA loss after post-r

Externí odkaz: https://doaj.org/article/47a0245915e84020b9ce3a42fe5784f1

RecG Directs DNA Synthesis during Double-Strand Break Repair.

Autor: Benura Azeroglu, Julia S P Mawer, Charlotte A Cockram, Martin A White, A M Mahedi Hasan, Milana Filatenkova, David R F Leach

Publikováno v: PLoS Genetics, Vol 12, Iss 2, p e1005799 (2016)

Homologous recombination provides a mechanism of DNA double-strand break repair (DSBR) that requires an intact, homologous template for DNA synthesis. When DNA synthesis associated with DSBR is convergent, the broken DNA strands are replaced and repa

Externí odkaz: https://doaj.org/article/db5fc476d63d47d28e62be6c530a41b4

Repair on the go: E. coli maintains a high proliferation rate while repairing a chronic DNA double-strand break.

Autor: Elise Darmon, John K Eykelenboom, Manuel A Lopez-Vernaza, Martin A White, David R F Leach

Publikováno v: PLoS ONE, Vol 9, Iss 10, p e110784 (2014)

DNA damage checkpoints exist to promote cell survival and the faithful inheritance of genetic information. It is thought that one function of such checkpoints is to ensure that cell division does not occur before DNA damage is repaired. However, in u

Externí odkaz: https://doaj.org/article/25178cb20c8741e0b49027a0e376de36

DNA targeting and interference by a bacterial Argonaute nuclease

Autor: Olga Maslova, David R. F. Leach, Alexei A. Aravin, D. S. Yudin, Mayya Petrova, Anton Kuzmenko, Sergei Ryazansky, Andrey Kulbachinskiy, Maria Ninova, Anastasiya Oguienko, A. G. Kudinova, Daria Esyunina

Publikováno v: Kuzmenko, A, Oguienko, A, Esyunina, D, Yudin, D, Petrova, M, Kudinova, A, Maslova, O, Ninova, M, Ryazansky, S, Leach, D, Aravin, A A & Kulbachinskiy, A 2020, ' DNA targeting and interference by a bacterial Argonaute nuclease ', Nature, vol. 587, pp. 632–637 . https://doi.org/10.1038/s41586-020-2605-1

Members of the conserved Argonaute protein family use small RNA guides to locate their mRNA targets and regulate gene expression and suppress mobile genetic elements in eukaryotes1,2. Argonautes are also present in many bacterial and archaeal species

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc28c961c82c2224784b1713d58137e8
https://pubmed.ncbi.nlm.nih.gov/32731256