Zobrazeno 1 - 10
of 25
pro vyhledávání: '"David Nishioka"'
Autor:
David Nishioka, Omid Vafa
Publikováno v:
Molecular Reproduction and Development. 40:36-47
A temporal study of protease expression employing the technique of SDS-PAGE gelatin substrate zymography revealed a definitive appearance of proteases during early development in the sea urchins, Lytechinus pictus and Strongylocentrotus purpuratus. T
Publikováno v:
The Journal of Cell Biology
An Mr 63-kD sea urchin sperm flagellar membrane protein has been previously implicated as a possible receptor for egg jelly ligand(s) that trigger the sperm acrosome reaction (AR). The cDNA and deduced amino acid sequences of the 63-kD protein are pr
Publikováno v:
Molecular Reproduction and Development. 28:410-418
Freeze-fracture electron microscopy reveals that intramembrane particles are concentrated in a band encircling the posterior portion of the acrosome of Strongylocentrotus purpuratus sperm. Two colloidal gold labeling methods, label-fracture and repli
Publikováno v:
Molecular Reproduction and Development. 27:181-190
Expression of the bindin gene was examined in testicular cells of the sea urchin Strongylocentrotus purpuratus. In situ hybridization studies, using an 35S-labeled antisense RNA probe transcribed from a bindin cDNA, reveal that bindin mRNAs are local
Publikováno v:
Cancer research. 63(12)
Demonstration of the existence of G-quadruplex structures in telomeres of Stylonychia macronuclei and in the promoter of c-myc in human cells has validated these secondary DNA structures as potential targets for drug design. The next important issue
Autor:
David, Nishioka, Vanessa, Marcell, Meghan, Cunningham, Merium, Khan, Daniel D, Von Hoff, Elzbieta, Izbicka
Publikováno v:
Methods in molecular medicine. 85
Publikováno v:
Differentiation; research in biological diversity. 60(3)
Previously, results were presented showing a spatiotemporal expression of matrix metalloproteases consistent with a role in remodeling the blastocoelic extracellular matrix (bECM) of the gastrulating sea urchin embryo [35]. In the present work, we pr
Publikováno v:
Zygote (Cambridge, England). 2(2)
SummarySpecific mRNAs coding for a 63 kDa sperm membrane protein (63-SMP) were localised in Strongylocentrotus purpuratus testis sections using in situ hybridisation techniques. 35S-labelled antisense RNA probes transcribed from a 766 base pair fragm
Publikováno v:
Developmental biology. 142(1)
This first study of the onset of spermatogenesis in the sea urchin, Strongylocentrotus purpuratus, was undertaken using individuals reared in the laboratory. Spermatogenesis commences about 11–12 months after metamorphosis in these animals. Bindin