Zobrazeno 1 - 10
of 25
pro vyhledávání: '"David N. Keys"'
Autor:
Paul J Gokhale, Janice K Au-Young, SriVidya Dadi, David N Keys, Neil J Harrison, Mark Jones, Shamit Soneji, Tariq Enver, Jon K Sherlock, Peter W Andrews
Publikováno v:
PLoS ONE, Vol 10, Iss 4, p e0123467 (2015)
We have used single cell transcriptome analysis to re-examine the substates of early passage, karyotypically Normal, and late passage, karyotypically Abnormal ('Culture Adapted') human embryonic stem cells characterized by differential expression of
Externí odkaz:
https://doaj.org/article/8ccf1997f49a4adda88675199717a01a
Autor:
Babu Valliyodan, Henry T. Nguyen, Jinrong Wan, David N. Keys, Yalei Wu, Caifu Chen, Li Song, J. Grover Shannon, Pius Brzoska
Publikováno v:
Advances in Bioscience and Biotechnology. :403-417
Detection and quantification of transgenes are important in analyzing genetically modified organisms (GMOs). Quantitative polymerase chain reaction (qPCR) is commonly utilized for such purposes. However, qPCR has certain limitations in detecting and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0b41d2e1b4fbe4cdb06af83f14569969
https://doi.org/10.4236/abb.2016.710039
https://doi.org/10.4236/abb.2016.710039
Publikováno v:
Journal of Cellular Physiology. 218:251-255
The unique properties of embryonic stem cells (ESCs) to self-renew indefinitely or to differentiate to any cell type have great potential for clinical applications in regenerative medicine. MicroRNAs (miRNAs) are emerging as important regulators of p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::688439ae4438498bd857b0a4fa6b78a7
https://doi.org/10.1002/jcp.21607
https://doi.org/10.1002/jcp.21607
Autor:
Sylvia Ahn, Hidetoshi Saiga, Daniel S. Rokhsar, Allen T. Christian, Noriyuki Satoh, Anna Di Gregorio, Byung In Lee, David N. Keys, Mei Wang, Paul G. Richardson, Sharon A. Doyle, Yutaka Satou, Cindy Zhang, Naoe Harafuji, Trevor Hawkins, Michael Levine, J. Chris Detter, Orsalem Kahsai
Publikováno v:
Proceedings of the National Academy of Sciences. 102:679-683
A screen for the systematic identification of cis-regulatory elements within large (>100 kb) genomic domains containing Hox genes was performed by using the basal chordate Ciona intestinalis . Randomly generated DNA fragments from bacterial artificia
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::58c9c4ee15aa7136180402137003dabb
https://doi.org/10.1073/pnas.0408952102
https://doi.org/10.1073/pnas.0408952102
Publikováno v:
Developmental Biology. 246:329-340
Dishevelled signaling plays a critical role in the control of cell intercalation during convergent extension in vertebrates. This study presents evidence that Dishevelled serves a similar function in the Ciona notochord. Embryos transgenic for mutant
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91b9633f10e5286443d3c8dbf987bcd4
https://doi.org/10.1006/dbio.2002.0656
https://doi.org/10.1006/dbio.2002.0656
Publikováno v:
Proceedings of the National Academy of Sciences. 99:6802-6805
Less than 100 cis-regulatory DNAs have been characterized in the context of transgenic metazoan embryos. Here we investigate the feasibility of conducting a genome-wide search for tissue-specific enhancers in the ascidian Ciona intestinalis . A total
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5933f4e0d42f136cb26350ea82b5e6ff
https://doi.org/10.1073/pnas.052024999
https://doi.org/10.1073/pnas.052024999
Autor:
Wijngaarden Pj, David N. Keys, Antónia Monteiro, Julie Gates, Paul M. Brakefield, Fanja Kesbeke, Sean B. Carroll
Publikováno v:
Nature. 384:236-242
The developmental and genetic bases for the formation, plasticity and diversity of eyespot patterns in butterflies are examined. Eyespot pattern mutants, regulatory gene expression, and transplants of the eyespot developmental organizer demonstrate t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7871fb04270795ee16495d588cf39691
https://doi.org/10.1038/384236a0
https://doi.org/10.1038/384236a0
Publikováno v:
Methods in Molecular Biology ISBN: 9781617794261
Quantification of the microRNAs (miRNAs) in cells or tissues is a crucial step in understanding their biological functions. Development of the stem-loop reverse transcription procedure and TaqMan(®) miRNA assays enables accurate detection of miRNA e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ce8f9a25776af458ddeea2ca27558f0e
https://doi.org/10.1007/978-1-61779-427-8_3
https://doi.org/10.1007/978-1-61779-427-8_3
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 632
TaqMan Array Cards are high-throughput, accurate, sensitive, and simple-to-use tools for quantitative analysis of mRNA or miRNA transcripts using a real-time PCR protocol. They utilize a microfluidic card with 384 reaction chambers and eight sample l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::62b22e2dbf0467b6e2c5124e8ebd1a93
https://pubmed.ncbi.nlm.nih.gov/20217573
https://pubmed.ncbi.nlm.nih.gov/20217573
Publikováno v:
Methods in Molecular Biology ISBN: 9781607616627
TaqMan Array Cards are high-throughput, accurate, sensitive, and simple-to-use tools for quantitative analysis of mRNA or miRNA transcripts using a real-time PCR protocol. They utilize a microfluidic card with 384 reaction chambers and eight sample l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::66e0b5e11495eb142f0a80f71bd0a4c5
https://doi.org/10.1007/978-1-60761-663-4_6
https://doi.org/10.1007/978-1-60761-663-4_6