Zobrazeno 1 - 9
of 9
pro vyhledávání: '"David Mark Whitcombe"'
Autor:
David Mark Whitcombe, Nicola Jo Thelwell, Stephen Little, Paul Francis Ravetto, Andrew Hughes, Caroline Dive, Ruth E Board, Malcolm R Ranson
Publikováno v:
Clinical Chemistry. 54:757-760
Background: Mutations in the PIK3CA gene (phosphoinositide-3-kinase, catalytic, alpha polypeptide) have recently been described in a number of cancers, and their detection is currently limited because of the low sensitivity of conventional sequencing
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42b1e2e16fcd6f8e1dd98a2474fae98a
https://doi.org/10.1373/clinchem.2007.098376
https://doi.org/10.1373/clinchem.2007.098376
Publikováno v:
Analytical Chemistry. 74:1069-1074
The accurate detection of DNA sequences is essential for a variety of post human genome projects including detection of specific gene variants for medical diagnostics and pharmacogenomics. A specific DNA sequence detection assay based on surface-enha
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e387eeeac200b24c5976e694925d4251
https://doi.org/10.1021/ac0155456
https://doi.org/10.1021/ac0155456
Autor:
Neil James Gibson, David Mark Whitcombe, Jayne C. Fox, Darren Hodgson, Peter Ceuppens, Simon J. Clayton, F Girdler, I Brotherick, David Browell, Carole A. Foy, Brian K. Shenton, Mary Stuart
Publikováno v:
Clinical Chemistry. 47:774-778
Alterations in the p53 gene have been reported in approximately one-half of human tumors (1). Much debate surrounds the clinical significance of p53 mutations as measured directly or inferred by immunohistochemical staining (2). Many studies have add
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f0169376e7054e5cc4501fb205fb6371
https://doi.org/10.1093/clinchem/47.4.774
https://doi.org/10.1093/clinchem/47.4.774
Publikováno v:
Current Opinion in Biotechnology. 9:602-608
The most tangible advances in DNA diagnostics during the past year have been in enhancing existing techniques to simplify their use and improve throughput. This has led to simplified genotyping methods using homogeneous analysis coupled with spectral
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26b8b66f148c80ca39e4ffd4b24f7671
https://doi.org/10.1016/s0958-1669(98)80137-7
https://doi.org/10.1016/s0958-1669(98)80137-7
Autor:
Stephen Little, Clive R. Newton, David Mark Whitcombe, Richard M. Ferrie, Jane Theaker, Jannine Brownie, Susan G. Shawcross
Publikováno v:
Nucleic Acids Research. 25:3235-3241
We attempted to produce primer-dimers (PDs) from a variety of primers with differing types and extents of complementarity. Where PDs were produced they were cloned and sequenced. We were unable to produce detectable PDs either with individual primers
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d91ae00d487bb51cf8ace7ab6c2f1390
https://doi.org/10.1093/nar/25.16.3235
https://doi.org/10.1093/nar/25.16.3235
Autor:
Stephen Little, Richard M. Ferrie, Clive R. Newton, Neil James Gibson, Helen L. Gillard, David Mark Whitcombe
Publikováno v:
Clinical Chemistry. 43:1336-1341
We combined the amplification refractory mutation system (ARMS™) and fluorescence polarization (FP) to give a homogeneous genomic DNA genotype analysis method. Oligonucleotide probes labeled with the fluorescein dyes fluorescein isothiocyanate and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::460ebcdb6c4a3bfea755f1157e110c14
https://doi.org/10.1093/clinchem/43.8.1336
https://doi.org/10.1093/clinchem/43.8.1336
Autor:
Nicola Jo Thelwell, Rupert Gaut, Paul Francis Ravetto, David Mark Whitcombe, Jennifer Ferguson, Rachael Carters
Publikováno v:
Methods in Molecular Biology ISBN: 9781588297006
A number of probe systems exist for the real-time detection of PCR products. Scorpions are a unique method wherein primer and probe are combined in a single oligonucleotide. During the PCR, the probe element becomes linked directly to its complementa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b3de3cc212a0ad5e9a4a46e8b8f3e8aa
https://doi.org/10.1007/978-1-60327-040-3_8
https://doi.org/10.1007/978-1-60327-040-3_8
Publikováno v:
Nature biotechnology. 17(8)
Molecular diagnostics is progressing from low-throughput, heterogeneous, mostly manual technologies to higher throughput, closed-tube, and automated methods. Fluorescence is the favored signaling technology for such assays, and a number of techniques
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd27cac856ff8eeebe1e69e35691575e
https://pubmed.ncbi.nlm.nih.gov/10429248
https://pubmed.ncbi.nlm.nih.gov/10429248
Publikováno v:
ChemPhysChem. 2:746
Accurate multiplex genotyping of the mutational status of the cystic fibrosis gene with the sensitive and selective SERRS technique is reported. This new assay does not require separation of the various alleles and shows that SERRS is a realistic alt
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ed952d6fc0c7eb9ccbc04da63fd4807c
https://doi.org/10.1002/1439-7641(20011217)2:12<746::aid-cphc746>3.0.co
https://doi.org/10.1002/1439-7641(20011217)2:12<746::aid-cphc746>3.0.co