Zobrazeno 1 - 10
of 17
pro vyhledávání: '"David M. Shechner"'
Autor:
Timothy M. Locke, Rose Fields, Hayden Gizinski, George M. Otto, Melissa J.S. MacEwen, Domnita-Valeria Rusnac, Peixian He, David M. Shechner, Chris D. McGann, Matthew D. Berg, Judit Villen, Yasemin Sancak, Devin K. Schweppe
Publikováno v:
Cell Reports, Vol 43, Iss 11, Pp 114879- (2024)
Summary: Calcium ions play important roles in nearly every biological process, yet whole-proteome analysis of calcium effectors has been hindered by a lack of high-throughput, unbiased, and quantitative methods to identify protein-calcium engagement.
Externí odkaz:
https://doaj.org/article/83058aefa024485bafa97ed7f25ab95d
Autor:
Sahar Attar, Valentino E. Browning, Yuzhen Liu, Eva K. Nichols, Ashley F. Tsue, David M. Shechner, Jay Shendure, Joshua A. Lieberman, Shreeram Akilesh, Brian J. Beliveau
In situhybridization (ISH) is a powerful tool for investigating the spatial arrangement of nucleic acid targets in fixed samples. ISH is typically visualized using fluorophores to allow high sensitivity and multiplexing or with colorimetric labels to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4c020b5f019e69917adfade277be52b4
https://doi.org/10.1101/2023.01.30.526264
https://doi.org/10.1101/2023.01.30.526264
Autor:
Ashley F. Tsue, Evan E. Kania, Diana Q. Lei, Rose Fields, Christopher D. McGann, Elliot Hershberg, Xinxian Deng, Maryanne Kihiu, Shao-En Ong, Christine M. Disteche, Sita Kugel, Brian J. Beliveau, Devin K. Schweppe, David M. Shechner
Publikováno v:
bioRxiv
Throughout biology, RNA molecules form complex networks of molecular interactions that are central to their function, but remain challenging to investigate. Here, we introduce Oligonucleotide-mediated proximity-interactome MAPping (O-MAP), a straight
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6883d23e364b652de865445cd891798e
https://europepmc.org/articles/PMC9882335/
https://europepmc.org/articles/PMC9882335/
Autor:
David M Shechner
Publikováno v:
Methods in Molecular Biology ISBN: 9781071606865
CRISPR-Display uses the S. pyogenes Cas9 protein to posttranscriptionally localize noncoding RNA (ncRNA) domains to any genomic site, by directly fusing these domains to the Cas9 sgRNA cofactor. This versatile technology enables numerous applications
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::564bb385631eeae47df2be2b42652f49
https://doi.org/10.1007/978-1-0716-0687-2_8
https://doi.org/10.1007/978-1-0716-0687-2_8
Autor:
David M, Shechner
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2162
CRISPR-Display uses the S. pyogenes Cas9 protein to posttranscriptionally localize noncoding RNA (ncRNA) domains to any genomic site, by directly fusing these domains to the Cas9 sgRNA cofactor. This versatile technology enables numerous applications
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::d03bc5bc0fbc379671fe69eaf27d076c
https://pubmed.ncbi.nlm.nih.gov/32926381
https://pubmed.ncbi.nlm.nih.gov/32926381
Autor:
Mark H. Ellisman, David M Shechner, Jeffrey D. Martell, Yisu Han, Daniela Boassa, Tess C. Branon, Alice Y. Ting
Publikováno v:
ACS chemical biology, vol 14, iss 4
APEX is an engineered peroxidase that catalyzes the oxidation of a wide range of substrates, facilitating its use in a variety of applications from subcellular staining for electron microscopy to proximity biotinylation for spatial proteomics and tra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cbe3de57ad363e46d1f0445952f7818b
https://europepmc.org/articles/PMC6548188/
https://europepmc.org/articles/PMC6548188/
Autor:
Daniela Boassa, David M Shechner, Tess C. Branon, Mark H. Ellisman, Yisu Han, Jeffrey D. Martell, Alice Y. Ting
APEX is an engineered peroxidase that catalyzes the oxidation of a wide range of substrates, facilitating its use in a variety of applications, from subcellular staining for electron microscopy to proximity biotinylation for spatial proteomics and tr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0279e6ceabd6d2464e6d6195aa7e383
Autor:
Chinmay J. Shukla, Chiara Gerhardinger, John L. Rinn, David M Shechner, Keegan Korthauer, Rafael A. Irizarry, Alexandra L McCorkindale, Philipp G. Maass, Moran N. Cabili
Publikováno v:
The EMBO Journal
In the post‐genomic era, thousands of putative noncoding regulatory regions have been identified, such as enhancers, promoters, long noncoding RNAs (lncRNAs), and a cadre of small peptides. These ever‐growing catalogs require high‐throughput as
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17df7f475dfa882e32f12631a7cc2da8
http://europepmc.org/articles/PMC5852646
http://europepmc.org/articles/PMC5852646
Publikováno v:
eLife, Vol 6 (2017)
eLife
eLife
The spatial organization of RNA within cells is a crucial factor in a wide range of biological functions, spanning all kingdoms of life. However, a general understanding of RNA localization has been hindered by a lack of simple, high-throughput metho
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::139aaa5ec3029eefd2204c214b254ce1
https://doi.org/10.7554/elife.29224
https://doi.org/10.7554/elife.29224