Hypoxia due to cardiac arrest induces a time-dependent increase in serum amyloid β levels in humans.

Autor: Henrik Zetterberg, Erik Mörtberg, Linan Song, Lei Chang, Gail K Provuncher, Purvish P Patel, Evan Ferrell, David R Fournier, Cheuk W Kan, Todd G Campbell, Ray Meyer, Andrew J Rivnak, Brian A Pink, Kaitlin A Minnehan, Tomasz Piech, David M Rissin, David C Duffy, Sten Rubertsson, David H Wilson, Kaj Blennow

Publikováno v: PLoS ONE, Vol 6, Iss 12, p e28263 (2011)

Amyloid β (Aβ) peptides are proteolytic products from amyloid precursor protein (APP) and are thought to play a role in Alzheimer disease (AD) pathogenesis. While much is known about molecular mechanisms underlying cerebral Aβ accumulation in fami

Externí odkaz: https://doaj.org/article/bd279e68e5df447f8caafa35e86ed2e2

Digital enzyme-linked immunosorbent assays with sub-attomolar detection limits based on low numbers of capture beads combined with high efficiency bead analysis

Autor: Danielle M. Svancara, Carmen I. Tobos, Roger Millington, Ray E. Meyer, Nicholas Collier, Alexander D. Wiener, David M. Rissin, Anna Comperchio, Christopher Warwick, Cheuk W. Kan, David C. Duffy

Publikováno v: Lab on a Chip. 20:2122-2135

We report the development of digital enzyme-linked immunosorbent assays (ELISAs) based on single molecule arrays (Simoa) with improved sensitivities over conventional digital ELISA, enabling detection of proteins at sub-attomolar concentrations. The

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57418cfee56df61d5c2c03bbd19fe573
https://doi.org/10.1039/d0lc00267d

Direct Detection of miR-122 in Hepatotoxicity Using Dynamic Chemical Labeling Overcomes Stability and isomiR Challenges

Autor: James W. Dear, Eric Boitier, Salvatore Pernagallo, David C. Duffy, Sébastien Laurent, Emma E. Morrison, Richard J. Mellanby, Lucile Sautier, Juan J. Díaz-Mochón, Aude Lartigau, David M. Rissin, John D. Tranter, Hugh Ilyine, Jean-François Léonard, Pedro Carmona-Sáez, Barbara López-Longarela, Lianne Chahman-Vos, Jordi Martorell-Marugán, Jean-Charles Gautier

Publikováno v: López-longarela, B, Morrison, E E, Tranter, J D, Chahman-vos, L, Léonard, J, Gautier, J, Laurent, S, Lartigau, A, Boitier, E, Sautier, L, Carmona-saez, P, Martorell-marugan, J, Mellanby, R J, Pernagallo, S, Ilyine, H, Rissin, D M, Duffy, D C, Dear, J W & Díaz-mochón, J J 2020, ' Direct Detection of miR-122 in Hepatotoxicity Using Dynamic Chemical Labeling Overcomes Stability and isomiR Challenges ', Analytical Chemistry . https://doi.org/10.1021/acs.analchem.9b05449

Circulating microRNAs are biomarkers reported to be stable and translational across species. MicroRNA-122 (miR-122) is a hepatocyte-specific microRNA biomarker for drug-induced liver injury (DILI). We developed a single molecule, dynamic chemical lab

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::355ebb0b8c10eadda0ff4309e718d045
https://hdl.handle.net/20.500.11820/6a3d708a-fc7c-4d13-939a-e1fedda53e81

Sensitivity and binding kinetics of an ultra-sensitive chemiluminescent enzyme-linked immunosorbent assay at arrays of antibodies

Autor: Shuai Nie, Carmen I. Tobos, Ki-Joo Sung, Seunghyeon Kim, Susan Yan, Joseph M. Johnson, David C. Duffy, Hadley D. Sikes, Bradley Rice, Scott Douglas, David M. Rissin

Publikováno v: Journal of immunological methods. 474

We have characterized the sensitivity and kinetics of a multiplex immunoassay system based on detection of chemiluminescence (CL) at arrays of antibodies. This enzyme-linked immunosorbent assay (ELISA) was based on the spotting of different antibodie

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d53c3a662c1c666d581df18ac2575a0b
https://pubmed.ncbi.nlm.nih.gov/31401067

A fully-automated, six-plex single molecule immunoassay for measuring cytokines in blood

Autor: Cheuk W. Kan, David C. Duffy, David M. Rissin, Todd G. Campbell, Derek R. DuPont, Andrew J. Rivnak, Carlos Cabrera, David Fournier, Sean Sullivan, Melissa Gardel, Brian A. Pink, Matthew W. Fishburn, Tomasz Piech, Linan Song

Publikováno v: Journal of Immunological Methods. 424:20-27

We report a system and assay for performing fully-automated measurement of 6 proteins simultaneously with single molecule sensitivity. The system combines handling of samples, reagents, and consumables, with a module for imaging single molecule array

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::584185c8c2ca6739c7ccbcfa74dad842
https://doi.org/10.1016/j.jim.2015.04.017