A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation.

Autor: Huaying Zhao, Rodolfo Ghirlando, Carlos Alfonso, Fumio Arisaka, Ilan Attali, David L Bain, Marina M Bakhtina, Donald F Becker, Gregory J Bedwell, Ahmet Bekdemir, Tabot M D Besong, Catherine Birck, Chad A Brautigam, William Brennerman, Olwyn Byron, Agnieszka Bzowska, Jonathan B Chaires, Catherine T Chaton, Helmut Cölfen, Keith D Connaghan, Kimberly A Crowley, Ute Curth, Tina Daviter, William L Dean, Ana I Díez, Christine Ebel, Debra M Eckert, Leslie E Eisele, Edward Eisenstein, Patrick England, Carlos Escalante, Jeffrey A Fagan, Robert Fairman, Ron M Finn, Wolfgang Fischle, José García de la Torre, Jayesh Gor, Henning Gustafsson, Damien Hall, Stephen E Harding, José G Hernández Cifre, Andrew B Herr, Elizabeth E Howell, Richard S Isaac, Shu-Chuan Jao, Davis Jose, Soon-Jong Kim, Bashkim Kokona, Jack A Kornblatt, Dalibor Kosek, Elena Krayukhina, Daniel Krzizike, Eric A Kusznir, Hyewon Kwon, Adam Larson, Thomas M Laue, Aline Le Roy, Andrew P Leech, Hauke Lilie, Karolin Luger, Juan R Luque-Ortega, Jia Ma, Carrie A May, Ernest L Maynard, Anna Modrak-Wojcik, Yee-Foong Mok, Norbert Mücke, Luitgard Nagel-Steger, Geeta J Narlikar, Masanori Noda, Amanda Nourse, Tomas Obsil, Chad K Park, Jin-Ku Park, Peter D Pawelek, Erby E Perdue, Stephen J Perkins, Matthew A Perugini, Craig L Peterson, Martin G Peverelli, Grzegorz Piszczek, Gali Prag, Peter E Prevelige, Bertrand D E Raynal, Lenka Rezabkova, Klaus Richter, Alison E Ringel, Rose Rosenberg, Arthur J Rowe, Arne C Rufer, David J Scott, Javier G Seravalli, Alexandra S Solovyova, Renjie Song, David Staunton, Caitlin Stoddard, Katherine Stott, Holger M Strauss, Werner W Streicher, John P Sumida, Sarah G Swygert, Roman H Szczepanowski, Ingrid Tessmer, Ronald T Toth, Ashutosh Tripathy, Susumu Uchiyama, Stephan F W Uebel, Satoru Unzai, Anna Vitlin Gruber, Peter H von Hippel, Christine Wandrey, Szu-Huan Wang, Steven E Weitzel, Beata Wielgus-Kutrowska, Cynthia Wolberger, Martin Wolff, Edward Wright, Yu-Sung Wu, Jacinta M Wubben, Peter Schuck

Publikováno v: PLoS ONE, Vol 10, Iss 5, p e0126420 (2015)

Analytical ultracentrifugation (AUC) is a first principles based method to determine absolute sedimentation coefficients and buoyant molar masses of macromolecules and their complexes, reporting on their size and shape in free solution. The purpose o

Externí odkaz: https://doaj.org/article/3bd91e8f42734f39965350067490e003

Comparative Thermodynamics of the Reversible Self-Association of Therapeutic mAbs Reveal Opposing Roles for Linked Proton- and Ion-Binding Events

Autor: Mandi M. Hopkins, Ioanna H. Antonopoulos, Arun Parupudi, Jared S. Bee, David L. Bain

Publikováno v: Pharmaceutical Research.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::da320722361a9123c0f889d8b8662f64
https://doi.org/10.1007/s11095-023-03485-1

Energetic Dissection of Mab-Specific Reversible Self-Association Reveals Unique Thermodynamic Signatures

Autor: Mandi M, Hopkins, Arun, Parupudi, Jared S, Bee, David L, Bain

Publikováno v: Pharmaceutical research. 38(2)

Reversible self-association (RSA) remains a challenge in the development of therapeutic monoclonal antibodies (mAbs). We recently analyzed the energetics of RSA for five IgG mAbs (designated as A-E) under matched conditions and using orthogonal metho

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::47a23da670d6024ff01f90482a18d99a
https://pubmed.ncbi.nlm.nih.gov/33604786

An Opportunity for Industry–Academia Partnership: Training the Next Generation of Industrial Researchers in Characterizing Higher Order Protein Structure

Autor: Christopher J. Roberts, David L. Bain, Michael Brenowitz

Publikováno v: Journal of Pharmaceutical Sciences. 105:3483-3486

Training researchers for positions in the United States biopharmaceutical industry has long been driven by academia. This commentary explores how the changing landscape of academic training will impact the industrial workforce, particularly with rega

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f9fff50dea6ba24efc5cb54e4610a5be
https://doi.org/10.1016/j.xphs.2016.08.007

Determination of Interaction Parameters for Reversibly Self-Associating Antibodies: A Comparative Analysis

Autor: Cherie L. Lambert, Jared S. Bee, Mandi M. Hopkins, David L. Bain, Arun Parupudi

Publikováno v: Journal of pharmaceutical sciences. 107(7)

Monoclonal antibodies (mAbs) represent a major class of biotherapeutics and are the fastest growing category of biologic drugs on the market. However, mAb development and formulation are often impeded by reversible self-association (RSA), defined as

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a7a59e21e98152688696bf6edd8b7ef
https://pubmed.ncbi.nlm.nih.gov/29571738