Zobrazeno 1 - 10
of 11
pro vyhledávání: '"David L, Wong"'
Autor:
Grant Ganshaw, Collier Katherine, James T. Kellis, Gudrun Vogtentanz, Brandy C. Falcon, Cindy S. Lai, Michael Bodo, David A. Estell, David L. Wong, Renato Meneses, Hans de Nobel, Marc Kolkman, Jeffrey V. Miller, Brian F. Schmidt, Scott Power, Walter Weyler, Alisha S. Jarnagin, Judy H. Chang, Anthony G. Day
Publikováno v:
Protein Expression and Purification. 55:40-52
A fusion protein based expression system was developed in the Gram-positive bacterium Bacillus subtilis to produce the soybean Bowman-Birk protease inhibitor (sBBI). The N-terminus of the mature sBBI was fused to the C-terminus of the 1st cellulose b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13120eaa96b31ac81e2b5f2ae9f53cb9
https://doi.org/10.1016/j.pep.2007.05.001
https://doi.org/10.1016/j.pep.2007.05.001
Autor:
Minha Park, Naoya Tsurushita, Bryan P. Fox, Judith A. Fox, Hendrik J. Meerman, Robin Fong, Victoria Doreen, David L. Wong, Huaming Wang, Christine Gieswein, Meng H. Heng, Jeff Palo Alto Pucci, Cherry Lin, Michael Ward
Publikováno v:
Applied and environmental microbiology. 70(5)
Two different humanized immunoglobulin G1(κ) antibodies and an Fab′ fragment were produced byAspergillus niger. The antibodies were secreted into the culture supernatant. Both light and heavy chains were initially synthesized as fusion proteins wi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b15d4d5b185e91fbed3a7030709fd678
https://pubmed.ncbi.nlm.nih.gov/15128505
https://pubmed.ncbi.nlm.nih.gov/15128505
Autor:
Alfred L. Gaertner, Beth Fryksdale, Paul T. Jedrzejewski, Sigrid Paech, Nicole Chow, Brian S. Miller, David L. Wong
Publikováno v:
Methods in Proteome and Protein Analysis ISBN: 9783642057793
With the availability of microbial and mammalian genomes combined with dramatic improvements in bioanalytical methods, high-throughput analysis of transcriptomes and proteomes has become a reality for academic and industrial laboratories alike. New t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0079c9ee9b2b1aaa8f9d80535315791a
https://doi.org/10.1007/978-3-662-08722-0_25
https://doi.org/10.1007/978-3-662-08722-0_25
Publikováno v:
Electrophoresis. 24(19-20)
Prefractionation of protein samples prior to two-dimensional electrophoresis (2-DE) has the potential to increase the dynamic detection range for proteomic analysis. We evaluated a membrane-based electrophoretic separation technique (Gradiflow) for i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d63dcea2415e853bbf8c8b5ed3b52bed
https://pubmed.ncbi.nlm.nih.gov/14595695
https://pubmed.ncbi.nlm.nih.gov/14595695
Publikováno v:
Electrophoresis. 23(14)
Glycosylation is a common post-translational modification that can add complexity to the proteome of many cell types. We used enzymatic and chemical methods of deglycosylation to treat a heavily glycosylated exoproteome sample from the filamentous fu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::4b81ef58a39c443f0ce9e6541369d37a
https://pubmed.ncbi.nlm.nih.gov/12210222
https://pubmed.ncbi.nlm.nih.gov/12210222
Autor:
David L. Wong, Norbert O. Reich
Publikováno v:
Biochemistry. 39(50)
We describe a highly sensitive strategy combining laser-induced photo-cross-linking and HPLC-based electrospray ionization mass spectrometry to identify amino acid residues involved in protein-DNA recognition. The photoactivatible cross-linking thymi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a4b0619e6d56a4d0edbeb65ff96a61f4
https://pubmed.ncbi.nlm.nih.gov/11112526
https://pubmed.ncbi.nlm.nih.gov/11112526
Autor:
Ricardo A. Garcia, Barrett W. Allan, Karen A. Maegley, Jessica L. Mort, William M. Lindstrom, David L. Wong, Joseph M. Beechem, Norbert O. Reich
Publikováno v:
The Journal of biological chemistry. 274(27)
EcoRI DNA methyltransferase was previously shown to bend its cognate DNA sequence by 52 degrees and stabilize the target adenine in an extrahelical orientation. We describe the characterization of an EcoRI DNA methyltransferase mutant in which histid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e994397e9fcce77da015fc22cdb9ce6
https://pubmed.ncbi.nlm.nih.gov/10383435
https://pubmed.ncbi.nlm.nih.gov/10383435
Publikováno v:
Nucleic acids research. 26(2)
We describe a novel strategy combining photocrosslinking and HPLC-based electrospray ionization mass spectrometry to identify UV crosslinked DNA-protein complexes. Eco RI DNA methyltransferase modifies the second adenine within the recognition sequen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4baf64d38ac2c5547b3ef9f95b4a0462
https://pubmed.ncbi.nlm.nih.gov/9421528
https://pubmed.ncbi.nlm.nih.gov/9421528
Publikováno v:
BMC Immunology. 5:12
We have devised a simple and efficient fluorescence-based method to track antigen uptake and processing in human B lymphoblastoid cells (B-LCL). Fluorescein labelled subtilisin was used to optimize antigen uptake conditions and identify processed pep
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5d678623e1001628d6e580db2f3f5e11
https://doi.org/10.1186/1471-2172-5-12
https://doi.org/10.1186/1471-2172-5-12
Publikováno v:
ELECTROPHORESIS. 23:2184
Glycosylation is a common post-translational modification that can add complexity to the proteome of many cell types. We used enzymatic and chemical methods of deglycosylation to treat a heavily glycosylated exoproteome sample from the filamentous fu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c206a936b8cd56aff4ee8f4d8923b38f
https://doi.org/10.1002/1522-2683(200207)23:14<2184::aid-elps2184>3.0.co
https://doi.org/10.1002/1522-2683(200207)23:14<2184::aid-elps2184>3.0.co