Zobrazeno 1 - 10
of 124
pro vyhledávání: '"David L, Hacker"'
Autor:
Mateja Manček-Keber, Florence Pojer, Alexander Herrmann, Tina Fink, Vida Forstnerič, Gregor Anderluh, Duško Lainšček, Roman Jerala, David L. Hacker, Hana Esih, Peter Pečan, Lucija Kadunc, Tjaša Plaper, Žiga Strmšek, Neža Omersa, Špela Malenšek, Andreas Pichlmair, Petra Dekleva, Mojca Benčina, Bruno E. Correia, David Peterhoff, Kelvin Lau, Jana Aupič, Sara Vidmar, Valter Bergant, Sara Orehek, Iva Hafner-Bratkovič, Ralf Wagner
Publikováno v:
Vaccines, Vol 9, Iss 431, p 431 (2021)
Vaccines
Volume 9
Issue 5
Vaccines
Volume 9
Issue 5
The response of the adaptive immune system is augmented by multimeric presentation of a specific antigen, resembling viral particles. Several vaccines have been designed based on natural or designed protein scaffolds, which exhibited a potent adaptiv
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6133a91e93c20c845908c38f717426d2
https://www.mdpi.com/2076-393X/9/5/431
https://www.mdpi.com/2076-393X/9/5/431
Autor:
Semira Gonseth Nusslé, Antony Croxatto, Murielle Bochud, Cyril Andre, Florence Pojer, Kelvin Lau, Jérémy Campos, Alix T. Coste, Berend Jan Bosch, Alex Farina, Didier Trono, Céline Pellaton, Gilbert Greub, Giuseppe Pantaleo, David L. Hacker, Craig Fenwick, Valérie D'Acremont
Publikováno v:
Journal of Virology
Journal of virology, vol. 95, no. 3, pp. e01828-20
Journal of virology, vol. 95, no. 3, pp. e01828-20
In the present study, we have determined SARS-CoV-2-specific antibody responses in sera of acute and postinfection phase subjects. Our results indicate that antibody responses against viral S and N proteins were equally sensitive in the acute phase o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb7a1532637321c375937782bcba31e9
http://europepmc.org/articles/PMC7925109
http://europepmc.org/articles/PMC7925109
Structural investigation of ACE2 dependent disassembly of the trimeric SARS-CoV-2 Spike glycoprotein
Autor:
Frank S. Lehmann, Kelvin Lau, David L. Hacker, Florence Pojer, Dongchun Ni, Henning Stahlberg, Andri Fraenkli
The human membrane protein Angiotensin-converting enzyme 2 (hACE2) acts as the main receptor for host cells invasion of the new coronavirus SARS-CoV-2. The viral surface glycoprotein Spike binds to hACE2, which triggers virus entry into cells. As of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d2b4bb182e316644d779a1b666373ab
https://doi.org/10.1101/2020.10.12.336016
https://doi.org/10.1101/2020.10.12.336016
Autor:
David L, Hacker, Paloma, Ordóñez-Morán
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2171
The presence of the proteins mouse R-Spondin1 (mRSpo1) and mouse Noggin (mNoggin) in a 3D-organoid culture allows for the maintenance of intestinal stem cells. Here, we describe a transient gene expression method for the production of these proteins
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e3011b815044d652d154b16663ff6a30
https://pubmed.ncbi.nlm.nih.gov/32705641
https://pubmed.ncbi.nlm.nih.gov/32705641
Autor:
Semira Gonseth Nusslé, Berend Jan Bosch, Alex Farina, Florence Pojer, Kelvin Lau, Giuseppe Pantaleo, David L. Hacker, Céline Pellaton, Cyril Andre, Jérémy Campos, Alix T. Coste, Valerie D'Acremont Genton, Antony Croxatto, Didier Trono, Murielle Bochud, Craig Fenwick, Gilbert Greub
We have determined SARS-CoV-2-specific antibody responses in a cohort of 96 individuals with acute infection and in 578 individuals enrolled in a seroprevalence population study in Switzerland including three groups, i.e. subjects with previous RT-PC
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd5196353ad94aca6a9595913cdc3484
Autor:
David L. Hacker, Paloma Ordóñez-Morán
Publikováno v:
Methods in Molecular Biology ISBN: 9781071607466
The presence of the proteins mouse R-Spondin1 (mRSpo1) and mouse Noggin (mNoggin) in a 3D-organoid culture allows for the maintenance of intestinal stem cells. Here, we describe a transient gene expression method for the production of these proteins
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6c89e0fbc3a6fd7771221c1c25d15469
https://doi.org/10.1007/978-1-0716-0747-3_10
https://doi.org/10.1007/978-1-0716-0747-3_10
Autor:
Wang Yukui, Zhen-long Wang, Florian M. Wurm, Dominique T. Monteil, Maria J Wurm, Xiaobin Li, David L. Hacker, Boyan Song, Li-kuan Zhu
Publikováno v:
Biochemical Engineering Journal. 129:84-95
Orbitally shaken bioreactors (OSRs) are commonly used for the cultivation of mammalian cells in suspension. Here we conducted a three-dimensional computational fluid dynamics (CFD) simulation to characterize the fluid field in the disposable 600-mL o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ac617eb92820f140744f7ad095044c6b
https://doi.org/10.1016/j.bej.2017.10.019
https://doi.org/10.1016/j.bej.2017.10.019
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1850
Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells are the two most popular mammalian hosts for the production of recombinant proteins. In this chapter the suspension cultivation and transfection of these cells in small-scale d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e9c3a83e0c433f1967531d43a7f65566
https://pubmed.ncbi.nlm.nih.gov/30242684
https://pubmed.ncbi.nlm.nih.gov/30242684