Zobrazeno 1 - 6
of 6
pro vyhledávání: '"David K. Walburger"'
Publikováno v:
Scanning. 18:407-416
Apoptosis is an important form of physiologic cell death displayed by an enormous variety of tissues under divergent conditions. The recent attention toward apoptosis in virtually all aspects of modern biology indicates that rapid and accurate differ
Autor:
Silvia Sanders, Irina A. Afonina, Robert Welch, David K. Walburger, Alan Mills, Sunita Yadavalli, Robert O. Dempcy, Alena Kulchenko, Yevgeniy S. Belousov, Cynthia L. Glaser, Walt Mahoney, Nicolaas M. J. Vermeulen
Publikováno v:
Human Genomics, Vol 1, Iss 3, Pp 209-217 (2004)
Human Genomics
Human Genomics
Probe and primer design for single nucleotide polymorphism (SNP) detection can be very challenging for A-T DNA-rich targets, requiring long sequences with lower specificity and stability, while G-C-rich DNA targets present limited design options to l
Autor:
David K. Walburger, Haley B. Skaer, James C. Diggans, Mojdeh Mohtashemi, Felicia N Sutton, Matthew W. Peterson
Publikováno v:
BMC Bioinformatics
BioMed Central
BMC Bioinformatics, Vol 12, Iss 1, p 314 (2011)
BioMed Central
BMC Bioinformatics, Vol 12, Iss 1, p 314 (2011)
Background Current biosensors are designed to target and react to specific nucleic acid sequences or structural epitopes. These 'target-specific' platforms require creation of new physical capture reagents when new organisms are targeted. An 'open-ta
Publikováno v:
2010 IEEE Sensors Applications Symposium (SAS).
Conventional microarray-based biosensors can only detect a limited number of organisms, and adding sensor capabilities requires re-engineering of reagents and devices to detect the presence of a novel microbial organism. To overcome these limitations
Autor:
Eugeny A. Lukhtanov, Vladimir V. Gorn, Michael W. Reed, Irina Shishkina, David K. Walburger, Irina A. Afonina, Yevheniy S. Belousov, Sergey G. Lokhov, Silvia Sanders, Merl F. Hoekstra, Nicolaas M. J. Vermeulen
Publikováno v:
SPIE Proceedings.
Fluorogenic 2'-deoxynucleotide probes containing a minor groove binding-quencher compound at the 5'-end and a fluorophore at the 3'-end, were recently described. These probes fluoresce upon hybridization to the complementary target. The 5'-MGB-quench
Autor:
Michael J. Singer, Joe Hedgpeth, Irina A. Afonina, Robert O. Dempcy, Sergey G. Lokhov, Michael W. Reed, Rich B. Meyer, Evgeniy S. Belousov, Eugeny A. Lukhtanov, David K. Walburger, Alan Mills, Vladimir V. Gorn, Igor V. Kutyavin, Alexander A. Gall
Publikováno v:
Nucleic acids research. 28(2)
DNA probes with conjugated minor groove binder (MGB) groups form extremely stable duplexes with single-stranded DNA targets, allowing shorter probes to be used for hybridization based assays. In this paper, sequence specificity of 3'-MGB probes was e