Výsledky vyhledávání - "David K. Kovalic"

The Use of Next Generation Sequencing and Junction Sequence Analysis Bioinformatics to Achieve Molecular Characterization of Crops Improved Through Modern Biotechnology

Autor: Liang Guo, David K. Kovalic, Andre Silvanovich, Mingya Huang, Jeanna Groat, Jerry Hjelle, Qing Tian, Allen T. Christian, Lyle Ralston, Gary A. Bannon, Stephen R. Padgette, Nordine Cheikh, Yongpan Yan, Carl W. Garnaat

Publikováno v: The Plant Genome, Vol 5, Iss 3, Pp 149-163 (2012)

The assessment of genetically modifi ed (GM) crops for regulatory approval currently requires a detailed molecular characterization of the DNA sequence and integrity of the transgene locus. In addition, molecular characterization is a critical compon

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb586f1d0d613957d4c8e7107fa3fc2c
https://doi.org/10.3835/plantgenome2012.10.0026

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23S rRNA domain V, a fragment that can be specifically methylated in vitro by the ErmSF (TlrA) methyltransferase

Autor: Bernard Weisblum, David K. Kovalic, Hyung-Jong Jin, Robert B. Giannattasio

Publikováno v: Journal of Bacteriology. 176:6992-6998

The DNA sequence that encodes 23S rRNA domain V of Bacillus subtilis, nucleotides 2036 to 2672 (C. J. Green, G. C. Stewart, M. A. Hollis, B. S. Vold, and K. F. Bott, Gene 37:261-266, 1985), was cloned and used as a template from which to transcribe d

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d72aa96fe330f930489d94cf5dc82d07
https://doi.org/10.1128/jb.176.22.6992-6998.1994

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Characterization of Unique Small RNA Populations from Rice Grain

Autor: James K. Roberts, Edwards Allen, Liang Guo, David K. Kovalic, Xiao Yang, Tracey L. Reynolds, Yuanji Zhang, Sara E. Heisel

Publikováno v: PLoS ONE
PLoS ONE, Vol 3, Iss 8, p e2871 (2008)

Small RNAs (∼20 to 24 nucleotides) function as naturally occurring molecules critical in developmental pathways in plants and animals [1], [2]. Here we analyze small RNA populations from mature rice grain and seedlings by pyrosequencing. Using a cl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02f5016549957781de997e5b4843d803
http://europepmc.org/articles/PMC2518513

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Methylation of minimalist 23S rRNA sequences in vitro by ErmSF (TlrA) N-methyltransferase

Autor: David K. Kovalic, Bernard Weisblum, Robert B. Giannattasio

Publikováno v: Biochemistry. 34(48)

ermSF (synonym tlrA) from Streptomyces fradiae NRRL 2702 confers resistance to the macrolide-lincosamide- streptogramin type B (MLS) superfamily of antibiotics. ErmSF specifically methylates Bacillus subtilis 23S rRNA in vitro at A2085 (B. subtilis c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c056a97f02e1daac2079aff622c6ebd
https://pubmed.ncbi.nlm.nih.gov/7495815

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[17] Direct cloning of DNA fragments generated by PCR

Autor: Bernard Weisblum, David K. Kovalic

Publisher Summary The cloning of amplified DNA from polymerase chain reactions (PCR) has become an important task for many researchers because the popularity of the PCR has increased. Unfortunately, the difficulty often encountered in cloning these f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a0b8bb86fe562ca869cab50ece3682dc
https://doi.org/10.1016/s1043-9471(06)80095-9

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General method for direct cloning of DNA fragments generated by the polymerase chain reaction

Autor: Bernard Weisblum, Jin-Hwan Kwak, David K. Kovalic

Publikováno v: Nucleic acids research. 19(16)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f79f5523457d4b79a00ff6fd8737471
https://pubmed.ncbi.nlm.nih.gov/1886782

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