Zobrazeno 1 - 10
of 28
pro vyhledávání: '"David J. Kast"'
Autor:
David J. Kast, Silvia Jansen
Publikováno v:
European Journal of Cell Biology, Vol 102, Iss 4, Pp 151363- (2023)
In vitro reconstitution assays using purified actin have greatly improved our understanding of cytoskeletal dynamics and their regulation by actin-binding proteins. However, early purification methods consisted of harsh conditions to obtain pure acti
Externí odkaz:
https://doaj.org/article/30fa2bc9551b49d1848a86dd6ab01805
Autor:
David J. Kast, Roberto Dominguez
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
IRSp53 is a key regulator of filopodia formation and cell migration. Here, the authors elucidate a mechanism of phosphorylation-dependent inhibition of IRSp53 by 14-3-3, which impedes the interactions of IRSp53 with membranes and downstream cytoskele
Externí odkaz:
https://doaj.org/article/bbc58d89b3dc4cbe9c4e3713e91232cf
Autor:
Roberto Dominguez, David J. Kast
Publikováno v:
Molecular Biology of the Cell
Filopodia are actin-filled membrane protrusions that play essential roles in cell motility and cell–cell communication and act as precursors of dendritic spines. IRSp53 is an essential regulator of filopodia formation, which couples Rho-GTPase sign
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7c7e13a50767bf116a204966d54232f
https://doi.org/10.1091/mbc.e18-09-0600
https://doi.org/10.1091/mbc.e18-09-0600
Autor:
Roberto Dominguez, David J. Kast
Publikováno v:
Current biology : CB. 27(8)
Actin cytoskeleton dynamics play vital roles in most forms of intracellular trafficking by promoting the biogenesis and transport of vesicular cargoes. Mounting evidence indicates that actin dynamics and membrane-cytoskeleton scaffolds also have esse
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::619287c606b042f15d34882cd1425a6f
https://pubmed.ncbi.nlm.nih.gov/28441569
https://pubmed.ncbi.nlm.nih.gov/28441569
Autor:
Moritz Winterhoff, Christophe Ampe, Giorgio Scita, David J. Kast, Andrea Disanza, Hans-Michael Müller, Paola Marighetti, Francesca Milanesi, Joern Linkner, Salvatore Cortellino, Jan Faix, Roberto Dominguez, Dmitry S. Ushakov, Davy Waterschoot, Marie-France Carlier, Sara Bisi, Andrea Palamidessi, Walter Nickel, Guillaume Romet-Lemonne
Publikováno v:
The EMBO Journal; Vol 32
The EMBO Journal
The EMBO Journal
Filopodia explore the environment, sensing soluble and mechanical cues during directional motility and tissue morphogenesis. How filopodia are initiated and spatially restricted to specific sites on the plasma membrane is still unclear. Here, we show
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0017c1e4fd489c424fb6e5282345f91a
Publikováno v:
Biophysical Journal. 93(6):2083-2090
We have performed molecular dynamics simulations of the phosphorylated (at S-19) and the unphosphorylated 25-residue N-terminal phosphorylation domain of the regulatory light chain (RLC) of smooth muscle myosin to provide insight into the structural
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b17d900e06cbbb83e3fbc1b634a77b3
Autor:
David J. Kast, Roberto Dominguez
Publikováno v:
Autophagy. 11(9)
Nucleation promoting factors (NPFs) control the spatio-temporal activity of Arp2/3 complex in cells [1, 2]. Thus, WASP and the WAVE complex direct the formation of branched actin networks at the leading edge during cell motility and endo/exocytosis,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::728474fb61442d515bc377281b4797c0
https://pubmed.ncbi.nlm.nih.gov/26096974
https://pubmed.ncbi.nlm.nih.gov/26096974
Publikováno v:
Nature Communications. 5
Actin filament nucleation and branching by Arp2/3 complex is activated by nucleation-promoting factors (NPFs), whose C-terminal WCA region contains binding sites for actin (W) and Arp2/3 complex (CA). It is debated whether one or two NPFs are require
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4633c54bc8f79cab59a306467b5921d3
https://doi.org/10.1038/ncomms4308
https://doi.org/10.1038/ncomms4308
Autor:
Tatyana Svitkina, Roberto Dominguez, Yadaiah Madasu, Changsong Yang, Malgorzata Boczkowska, David J. Kast, Andrea Disanza, Giorgio Scita
Publikováno v:
Nature structuralmolecular biology. 21(4)
The Rho family GTPase effector IRSp53 has essential roles in filopodia formation and neuronal development, but its regulatory mechanism is poorly understood. IRSp53 contains a membrane-binding BAR domain followed by an unconventional CRIB motif that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3fdd08f5aa00efbb0c5ea285f25f901
https://pubmed.ncbi.nlm.nih.gov/24584464
https://pubmed.ncbi.nlm.nih.gov/24584464
Chromatin-remodeling complexes are assembled around a catalytic subunit that contains a central ATPase domain and flanking sequences that recruit auxiliary subunits. The catalytic subunits of SWI/SNF remodelers recruit Arp7/9 through a helicase/SANT-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28ad7f191a85718211132734da9d1206
https://europepmc.org/articles/PMC3861627/
https://europepmc.org/articles/PMC3861627/
