Zobrazeno 1 - 10
of 23
pro vyhledávání: '"David J, Wurm"'
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 11 (2023)
The two major scale-up criteria in continuously stirred bioreactors are 1) constant aerated power input per volume (Pg/Vl), and 2) the volumetric O2 mass transfer coefficient (kla). However, Pg/Vl is only influenced by the stirrer geometry, stirrer s
Externí odkaz:
https://doaj.org/article/fb674f43b8fc4ee0b0ab6bb4d2edb526
Publikováno v:
AMB Express, Vol 12, Iss 1, Pp 1-9 (2022)
Key points Development of a flow cytometry (FCM) method for viability determination of S. acidocaldarius using the fluorescent dyes fluorescein diacetate and concanavalin A conjugated with rhodamine. Applicability of the developed method was shown vi
Externí odkaz:
https://doaj.org/article/3c1b6f916d564927a6d8622e35464101
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 8 (2021)
To date, high-pressure homogenization is the standard method for cell disintegration before the extraction of cytosolic and periplasmic protein from E. coli. Its main drawback, however, is low selectivity and a resulting high load of host cell impuri
Externí odkaz:
https://doaj.org/article/ca3fb7276a994272919d2ed685e3a040
Autor:
Julian Kopp, Stefan Kittler, Christoph Slouka, Christoph Herwig, Oliver Spadiut, David J. Wurm
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)
Recombinant protein production with Escherichia coli is usually carried out in fed-batch mode in industry. As set-up and cleaning of equipment are time- and cost-intensive, it would be economically and environmentally favorable to reduce the number o
Externí odkaz:
https://doaj.org/article/cb141ed4f8e24e8e882c747741f62b93
Autor:
Christoph Slouka, David J. Wurm, Georg Brunauer, Andreas Welzl-Wachter, Oliver Spadiut, Jürgen Fleig, Christoph Herwig
Publikováno v:
Sensors, Vol 16, Iss 11, p 1900 (2016)
New approaches in process monitoring during industrial fermentations are not only limited to classical pH, dO2 and offgas analysis, but use different in situ and online sensors based on different physical principles to determine biomass, product qual
Externí odkaz:
https://doaj.org/article/04b0653d9036413d9b489b74bd596858
Publikováno v:
Frontiers in Bioengineering and Biotechnology
To date, high-pressure homogenization is the standard method for cell disintegration before the extraction of cytosolic and periplasmic protein from E. coli. Its main drawback, however, is low selectivity and a resulting high load of host cell impuri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::37fc1a2576ddfd471baf7da582d8a77f
https://pubmed.ncbi.nlm.nih.gov/33634078
https://pubmed.ncbi.nlm.nih.gov/33634078
Publikováno v:
International Journal of Molecular Sciences, Vol 21, Iss 3935, p 3935 (2020)
The microbial cell membrane is affected by physicochemical parameters, such as temperature and pH, but also by the specific growth rate of the host organism. Homeoviscous adaption describes the process of maintaining membrane fluidity and permeabilit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doajarticles::bfee0d6163968595c75da562dbe2adec
https://www.mdpi.com/1422-0067/21/11/3935
https://www.mdpi.com/1422-0067/21/11/3935
Autor:
Julia Mildner, David J. Wurm, Bernhard Lendl, Christoph Herwig, Andreas Schwaighofer, Karin Wieland, Britta Eggenreich, Christoph Slouka, Oliver Spadiut, Julian Quehenberger, Vignesh Rajamanickam
Publikováno v:
Applied Microbiology and Biotechnology
Against the outdated belief that inclusion bodies (IBs) in Escherichia coli are only inactive aggregates of misfolded protein, and thus should be avoided during recombinant protein production, numerous biopharmaceutically important proteins are curre
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::022e8c8664a95d0df0a38297b4a84037
https://doi.org/10.1007/s00253-017-8641-6
https://doi.org/10.1007/s00253-017-8641-6
Publikováno v:
Engineering in Life Sciences. 17:598-604
In a recently published study, we developed a simple methodology to monitor Escherichia coli cell integrity and lysis during bioreactor cultivations, where we intentionally triggered leakiness. In this follow-up study, we used this methodology, compr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c71bb74d8f636384fd3c15919461b172
https://doi.org/10.1002/elsc.201600204
https://doi.org/10.1002/elsc.201600204
Autor:
Oliver Spadiut, David J. Wurm
Publikováno v:
Methods in Molecular Biology ISBN: 9781493990238
Pichia pastoris is one of the most important host organisms for the recombinant production of proteins in industrial biotechnology. A prominent promoter system for recombinant protein production in P. pastoris is the promoter of alcohol oxidase (PAOX
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f65e0e765f957d12dffe24a3baafb15c
https://doi.org/10.1007/978-1-4939-9024-5_15
https://doi.org/10.1007/978-1-4939-9024-5_15
