Zobrazeno 1 - 10
of 12
pro vyhledávání: '"David Ivor Gwynne"'
Publikováno v:
Gene. 132(2)
A new expression-secretion system of Aspergillus niger which directs the secretion of heterologous proteins is described. The promoter and signal peptide-encoding region of the phosphate-repressible aphA gene of A. niger, when fused to the coding reg
Autor:
Sheila Garven, R.W. Davies, MacRae Wd, David Ivor Gwynne, Susan Sibley, Herbert N. Arst, Frank Buxton
Publikováno v:
Gene. 130(2)
A clone from an Aspergillus nidulans library was identified by its ability to confer enhanced staining for acid phosphatase (APase) activity upon phosphatase-deficient A. nidulans mutants. This APase activity is not repressed by high phosphate concen
Publikováno v:
Gene. 37:207-214
A mutant of Aspergillus niger defective in ornithine transcarbamylase function was transformed with plasmids carrying a functional copy of the argB gene of Aspergillus nidulans after treatment of spheroplasts in the presence of polyethylene glycol an
Autor:
Frank Buxton, David Ivor Gwynne, Elliott R, Lockington Ra, Claudio Scazzocchio, Pickett M, R.W. Davies, H.M. Sealy-Lewis
Publikováno v:
Gene. 51:217-226
We have cloned and sequenced the aldA (encoding aldehyde dehydrogenase) gene of Aspergillus nidulans . The gene contains two introns which are similar in size and structure to other fungal introns. The amino acid sequence of aldehyde dehydrogenase (4
Publikováno v:
Current Genetics. 14:561-566
We describe the preparation of an in vitro translation system from heat shock-treated Aspergillus nidulans, capable of supporting efficient and faithful synthesis of proteins from natural and in vitro transcribed eukaryotic messages. In vitro synthes
Autor:
Susan Sibley, Claudio Scazzocchio, Frank Buxton, David Ivor Gwynne, Robin A. Lockington, R. Wayne Davies, H.M. Sealy-Lewis
Publikováno v:
Gene. 51:205-216
The alcA and aldA genes of Aspergillus nidulans are regulated in exactly the same manner, being subject to positive control by the product of the alcR gene. We report the complete nucleotide sequence of the alcA gene and its 5′ non-coding region, p
Publikováno v:
Nature Biotechnology. 5:713-719
Secretion of biologically active human interferon α2 and a bacterial (Cellulomonas fimi) endoglucanase from Aspergillus nidulans is described. The genes encoding these proteins were linked to NH2–terminal fungal secretion signal sequences, synthet
Publikováno v:
Gene. 84(2)
Mutants that lack adenosine triphosphate sulfurylase (ATPsase; EC 2.7.7.4) are unable to use sulfate as sole source of sulfur and are also resistant to selenate. These mutants, denoted sC − , are readily obtained from any strain of Aspergillus nige
Publikováno v:
Gene. 60(2-3)
We have cloned the gene encoding ornithine carbamoyl transferase (OCTase) from Aspergillus niger. The structure and complete nucleotide sequence of this gene have been determined. The gene encodes an mRNA of 1.3 kb. The transcription unit contains an
Autor:
David Ivor Gwynne, Jagdeep K. Buch, A. Michael Sills, Frank Buxton, R. Wayne Davies, Deborah Drake, Shirley A. Williams, James A. Johnstone, Margit Westphal, Zimin Guo
Publikováno v:
Biochemical Society transactions. 17(2)