Zobrazeno 1 - 10
of 68
pro vyhledávání: '"David H Perlman"'
Autor:
Zia Khan, Joshua S Bloom, Sasan Amini, Mona Singh, David H Perlman, Amy A Caudy, Leonid Kruglyak
Publikováno v:
Molecular Systems Biology, Vol 8, Iss 1, Pp 1-12 (2012)
Abstract Understanding the genetic basis of gene regulatory variation is a key goal of evolutionary and medical genetics. Regulatory variation can act in an allele‐specific manner (cis‐acting) or it can affect both alleles of a gene (trans‐acti
Externí odkaz:
https://doaj.org/article/fb38a8f903814fb7852ab6f53283aa4c
Autor:
Harrison Specht, Edward Emmott, Aleksandra A. Petelski, R. Gray Huffman, David H. Perlman, Marco Serra, Peter Kharchenko, Antonius Koller, Nikolai Slavov
Publikováno v:
Genome Biology, Vol 22, Iss 1, Pp 1-27 (2021)
Abstract Background Macrophages are innate immune cells with diverse functional and molecular phenotypes. This diversity is largely unexplored at the level of single-cell proteomes because of the limitations of quantitative single-cell protein analys
Externí odkaz:
https://doaj.org/article/48c645d97c414b82abdbbbe683278eab
Autor:
R. Gray Huffman, Andrew Leduc, Christoph Wichmann, Marco Di Gioia, Francesco Borriello, Harrison Specht, Jason Derks, Saad Khan, Luke Khoury, Edward Emmott, Aleksandra A. Petelski, David H. Perlman, Jürgen Cox, Ivan Zanoni, Nikolai Slavov
Publikováno v:
Nature Methods. 20:714-722
Major aims of single-cell proteomics include increasing the consistency, sensitivity and depth of protein quantification, especially for proteins and modifications of biological interest. Here, to simultaneously advance all these aims, we developed p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c2cbe6263ec39b9b43e5265aae1afe91
https://doi.org/10.1038/s41592-023-01830-1
https://doi.org/10.1038/s41592-023-01830-1
Autor:
Rob C. Oslund, Tamara Reyes-Robles, Cory H. White, Jake H. Tomlinson, Kelly A. Crotty, Edward P. Bowman, Dan Chang, Vanessa M. Peterson, Lixia Li, Silvia Frutos, Miquel Vila-Perelló, David Vlerick, Karen Cromie, David H. Perlman, Sampat Ingale, Samantha D. O’ Hara, Lee R. Roberts, Grazia Piizzi, Erik C. Hett, Daria J. Hazuda, Olugbeminiyi O. Fadeyi
Publikováno v:
Nature Chemical Biology. 18:850-858
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ab69960405338ea714227347b00d561a
https://doi.org/10.1038/s41589-022-01044-0
https://doi.org/10.1038/s41589-022-01044-0
Autor:
Laurent Gatto, Ruedi Aebersold, Juergen Cox, Vadim Demichev, Jason Derks, Edward Emmott, Alexander M. Franks, Alexander R. Ivanov, Ryan T. Kelly, Luke Khoury, Andrew Leduc, Michael J. MacCoss, Peter Nemes, David H. Perlman, Aleksandra A. Petelski, Christopher M. Rose, Erwin M. Schoof, Jennifer Van Eyk, Christophe Vanderaa, John R. Yates, Nikolai Slavov
Publikováno v:
Nature Methods, Vol. 20, no.3, p. 375-386 (2023)
Nat Methods
Nat Methods
Analyzing proteins from single cells by tandem mass spectrometry (MS) has become technically feasible. While such analysis has the potential to accurately quantify thousands of proteins across thousands of single cells, the accuracy and reproducibili
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ffcf2355b99e372fc841c11cdcc6592
https://hdl.handle.net/2078.1/273466
https://hdl.handle.net/2078.1/273466
Autor:
Stephan Menne, Stefan Wieland, David H. Perlman, Peter Revill, Jianming Hu, Laurie Luckenbaugh, Xupeng Hong
Publikováno v:
Hepatology
BACKGROUND AND AIMS The hepatitis B core-related antigen (HBcrAg), a composite antigen of precore/core gene including classical hepatitis B core protein (HBc) and HBeAg and, additionally, the precore-related antigen PreC, retaining the N-terminal sig
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::def0deced31a0331776f3eeb9344f387
https://doi.org/10.1002/hep.31720
https://doi.org/10.1002/hep.31720
Autor:
R Gray Huffman, Andrew Leduc, Christoph Wichmann, Marco di Gioia, Francesco Borriello, Harrison Specht, Jason Derks, Saad Khan, Luke Khoury, Edward Emmott, Aleksandra A. Petelski, David H Perlman, Jürgen Cox, Ivan Zanoni, Nikolai Slavov
Major aims of single-cell proteomics include increasing the consistency, sensitivity, and depth of protein quantification, especially for proteins and modifications of biological interest. To simultaneously advance all these aims, we developed priori
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5fa6871c587c9af505a64fa41b0578c6
https://doi.org/10.1101/2022.03.16.484655
https://doi.org/10.1101/2022.03.16.484655
Autor:
Grazia Piizzi, Karen Cromie, Samantha D. O’Hara, Rob C. Oslund, Kelly A. Crotty, Vanessa M. Peterson, Lee R. Roberts, Daria J. Hazuda, Cory H. White, Erik C. Hett, Tamara Reyes-Robles, David H. Perlman, Dan Chang, David Vlerick, Silvia Frutos, Olugbeminiyi O. Fadeyi, Edward P. Bowman, Jake H. Tomlinson, Miquel Vila-Perelló, Lixia Li
Cell-cell interactions drive essential biological processes critical to cell and tissue development, function, pathology, and disease outcome. The growing appreciation of immune cell interactions within disease environments has led to significant eff
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::be94bbf6f8e3c3e1350c37ea44680a31
https://doi.org/10.1101/2021.10.04.463039
https://doi.org/10.1101/2021.10.04.463039
Autor:
Harrison Specht, Edward Emmott, Aleksandra A. Petelski, R. Gray Huffman, David H. Perlman, Antonius Koller, Nikolai Slavov
Protocol for preparing single cells for mass-spec analysis by SCoPE2 as described by Specht et al., doi: 10.1101/665307 (2019) and Specht et al., Genome Biology, doi: 10.1186/s13059-021-02267-5 (2021). For a detailed protocol paper, see Petelski A, E
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c31f971b892eeedbf5c7d3a2910d4fb3
https://doi.org/10.17504/protocols.io.bwb3paqn
https://doi.org/10.17504/protocols.io.bwb3paqn
Autor:
R. Gray Huffman, Aleksandra A. Petelski, Andrew Leduc, Harrison Specht, Edward Emmott, David H. Perlman, Nikolai Slavov
Publikováno v:
Nature Protocols
Nat Protoc
Nat Protoc
Many biological systems are composed of diverse single cells. This diversity necessitates functional and molecular single-cell analysis. Single-cell protein analysis has long relied on affinity reagents, but emerging mass-spectrometry methods (either
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a48a61879c63746354df9dec3b721bc1
https://doi.org/10.1101/2021.03.12.435034
https://doi.org/10.1101/2021.03.12.435034