Zobrazeno 1 - 10
of 17
pro vyhledávání: '"David G. Whittingham"'
Publikováno v:
Biology of Reproduction. 69:1859-1862
Mouse spermatozoa from the caudae epididymides could be freeze-dried without losing their ability to support normal development. Immature spermatozoa from the testes, in contrast, were damaged by freeze-drying. However, immature spermatozoa became re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9c38af3d034be69f3591b6b01c523b58
https://doi.org/10.1095/biolreprod.103.019729
https://doi.org/10.1095/biolreprod.103.019729
Long-Term Preservation of Mouse Spermatozoa after Freeze-Drying and Freezing Without Cryoprotection1
Autor:
David G. Whittingham, Monika A. Ward, Hirokazu Kusakabe, John D. Biggers, Takehito Kaneko, Ryuzo Yanagimachi
Publikováno v:
Biology of Reproduction. 69:2100-2108
The widespread production of mice with transgenes, disrupted genes and mutant genes, has strained the resources available for maintaining these mouse lines as live populations, and dependable methods for gamete and embryo preservation in these lines
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c92367941429764827f3a4e9820d6acc
https://doi.org/10.1095/biolreprod.103.020529
https://doi.org/10.1095/biolreprod.103.020529
Publikováno v:
Biology of Reproduction. 68:136-139
The nuclei of freeze-dried mouse spermatozoa are able to retain their chromosome integrity and developmental potential. To optimize the conditions of freeze-drying, we examined whether pH values of the freeze-drying solution affect the chromosome int
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1cd5b23d18c39d7a2299cc468a5b10fb
https://doi.org/10.1095/biolreprod.102.008706
https://doi.org/10.1095/biolreprod.102.008706
Publikováno v:
Biology of Reproduction. 67:1278-1284
Efficient and dependable mouse cryopreservation methods are urgently needed because the production of mice with transgenes and disrupted and mutant genes is now commonplace. Preservation of these unique genomes provides an essential safeguard for fut
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a8cea704211ee1faba4b6bc6cd5639f2
https://doi.org/10.1095/biolreprod67.4.1278
https://doi.org/10.1095/biolreprod67.4.1278
Publikováno v:
Biology of Reproduction. 67:287-292
Success with in vitro fertilization (IVF) using inbred strains of mice varies considerably and appears to be related to the proportion of motile spermatozoa present in epididymal sperm samples of different strains. In this study, motile spermatozoa w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9c23d58898eacf829247a2b3640cd597
https://doi.org/10.1095/biolreprod67.1.287
https://doi.org/10.1095/biolreprod67.1.287
Publikováno v:
Proceedings of the National Academy of Sciences. 98:13501-13506
Chromosome stability was maintained in mouse spermatozoa after freeze-drying or freezing without cryoprotection in a simple Tris⋅HCl buffer containing EGTA (50 mM) and NaCl (50 mM). The ability of spermatozoa to activate oocytes spontaneously was n
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d57ab94ffdda8ddccce461de26c356e
https://doi.org/10.1073/pnas.241517598
https://doi.org/10.1073/pnas.241517598
Autor:
Audrey L. Muggleton-Harris, David G. Whittingham, Leroy Hood, Christopher M. Gomez, Carol Readhead
Publikováno v:
Proceedings of the National Academy of Sciences. 87:4481-4484
As a model for the detection of human genetic disease in preimplantation embryos, we describe a method in which trophectoderm biopsy samples from viable mouse blastocysts are simultaneously analyzed for the presence of a normal or mutant allele of th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e9643e8a6939629006318eb751afe2ac
https://doi.org/10.1073/pnas.87.12.4481
https://doi.org/10.1073/pnas.87.12.4481
Autor:
Monika A, Ward, Takehito, Kaneko, Hirokazu, Kusakabe, John D, Biggers, David G, Whittingham, Ryuzo, Yanagimachi
Publikováno v:
Biology of reproduction. 69(6)
The widespread production of mice with transgenes, disrupted genes and mutant genes, has strained the resources available for maintaining these mouse lines as live populations, and dependable methods for gamete and embryo preservation in these lines
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::ba571dd9a4ba2a778259bac12b44fc5d
https://pubmed.ncbi.nlm.nih.gov/12930716
https://pubmed.ncbi.nlm.nih.gov/12930716
Publikováno v:
Biology of reproduction. 69(6)
Mouse spermatozoa from the caudae epididymides could be freeze-dried without losing their ability to support normal development. Immature spermatozoa from the testes, in contrast, were damaged by freeze-drying. However, immature spermatozoa became re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::9c0bbfcc26413bc6b0e696f4d5ff8a47
https://pubmed.ncbi.nlm.nih.gov/12904320
https://pubmed.ncbi.nlm.nih.gov/12904320
Publikováno v:
Biology of reproduction. 68(1)
The nuclei of freeze-dried mouse spermatozoa are able to retain their chromosome integrity and developmental potential. To optimize the conditions of freeze-drying, we examined whether pH values of the freeze-drying solution affect the chromosome int
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::98442d5f364aa5c247a125f5b018c588
https://pubmed.ncbi.nlm.nih.gov/12493705
https://pubmed.ncbi.nlm.nih.gov/12493705