Zobrazeno 1 - 8
of 8
pro vyhledávání: '"David G. Schwark"'
Publikováno v:
Frontiers in Molecular Biosciences, Vol 9 (2022)
The extent to which alteration of endogenous tRNA modifications may be exploited to improve genetic code expansion efforts has not been broadly investigated. Modifications of tRNAs are strongly conserved evolutionarily, but the vast majority of E. co
Externí odkaz:
https://doaj.org/article/a5b239e32e264788a3f37dd61f807f9e
Publikováno v:
Frontiers in Chemistry, Vol 10 (2022)
The expansion of the genetic code beyond a single type of noncanonical amino acid (ncAA) is hindered by inefficient machinery for reassigning the meaning of sense codons. A major obstacle to using directed evolution to improve the efficiency of sense
Externí odkaz:
https://doaj.org/article/4d7c169826ad485bac71a0e7d3ef9377
Publikováno v:
International Journal of Molecular Sciences, Vol 22, Iss 2, p 895 (2021)
Genetic code expansion has largely focused on the reassignment of amber stop codons to insert single copies of non-canonical amino acids (ncAAs) into proteins. Increasing effort has been directed at employing the set of aminoacyl tRNA synthetase (aaR
Externí odkaz:
https://doaj.org/article/2409ed3f96d440f7857996dd901d5c11
Autor:
Y. Bill Kim, Elizabeth B. Pierce, Michael Brown, Brenda A. Peterson, Derek Sanford, Justin Fear, David Nicholl, Ellyce San Pedro, Grace M. Reynolds, Joanne E. Hunt, David G. Schwark, Sathya Jali, Nathaniel Graham, Zoe Cesarz, Tracey A. Lincoln Chapman, Joseph M. Watts, Aaron W. Hummel
CRISPR/Cas systems coupled with reverse transcriptase (RT), such as the recently described Prime editing, allow for site-specific replacement of DNA sequences. Despite widespread testing of Prime editing, it is currently only compatible with type II
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3c974a20bbc15459608b6ad51e81a78c
https://doi.org/10.1101/2022.12.13.520319
https://doi.org/10.1101/2022.12.13.520319
Autor:
Nathaniel D. Graham, David G. Schwark, Thomas J. Poorten, Fräbel Sabine, Jingyi Nie, Shai J. Lawit
Publikováno v:
Genome editing for precision crop breeding ISBN: 9781786764478
Genome editing for precision crop breeding
Genome editing for precision crop breeding
Base editors are gene editing tools that allow targeted nucleic acid conversions, most commonly C>T and A>G, through pairing of deamination domains with impaired nucleases. Multiple deaminase domains and architectures have been demonstrated in planta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::206394d5104eaf6e1a3ff1e7e16d8e60
https://doi.org/10.1201/9781003048237-6
https://doi.org/10.1201/9781003048237-6
Publikováno v:
International Journal of Molecular Sciences, Vol 22, Iss 895, p 895 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 2
International Journal of Molecular Sciences
Volume 22
Issue 2
Genetic code expansion has largely focused on the reassignment of amber stop codons to insert single copies of non-canonical amino acids (ncAAs) into proteins. Increasing effort has been directed at employing the set of aminoacyl tRNA synthetase (aaR
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4fdb1b82ee1aa99722c4c0505210467
https://www.mdpi.com/1422-0067/22/2/895
https://www.mdpi.com/1422-0067/22/2/895
Publikováno v:
Chembiochem : a European journal of chemical biology. 21(16)
A quantitative understanding of how system composition and molecular properties conspire to determine the fidelity of translation is lacking. Our strategy directs an orthogonal tRNA to directly compete against endogenous tRNAs to decode individual ta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4683e371f3d80a668f4ec551dc6ccc56
https://pubmed.ncbi.nlm.nih.gov/32203635
https://pubmed.ncbi.nlm.nih.gov/32203635
Publikováno v:
Genes
Non-canonical amino acids (ncAAs) are finding increasing use in basic biochemical studies and biomedical applications. The efficiency of ncAA incorporation is highly variable, as a result of competing system composition and codon context effects. The
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::eb4c73ea6be4693fdc7bfbfddfabd124
https://pubmed.ncbi.nlm.nih.gov/30424562
https://pubmed.ncbi.nlm.nih.gov/30424562