Zobrazeno 1 - 5
of 5
pro vyhledávání: '"David Estapé"'
Publikováno v:
Journal of Biotechnology. 127:244-257
During production in recombinant Escherichia coli, the human basic fibroblast growth factor (hFGF-2) partly aggregates into stable cytoplasmic inclusion bodies. These inclusion bodies additionally contain significant amounts of the heat-shock chapero
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d4fc078659905e8c2fdef399b3521c4
https://doi.org/10.1016/j.jbiotec.2006.07.004
https://doi.org/10.1016/j.jbiotec.2006.07.004
Publikováno v:
Biochemical Journal. 335:343-349
The conformational stability and the folding properties of the all-beta-type protein human basic fibroblast growth factor (hFGF-2) were studied by means of fluorescence spectroscopy. The results show that the instability of the biological activity of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::803d1024148a527c8c6bd722f0e2a5c3
https://doi.org/10.1042/bj3350343
https://doi.org/10.1042/bj3350343
Autor:
Anke Seeger, David Estapé
Publikováno v:
Single-Use Technology in Biopharmaceutical Manufacture
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::acb7b8e782dbd3f6c60decf08213c743
https://doi.org/10.1002/9780470909997.ch30
https://doi.org/10.1002/9780470909997.ch30
Publikováno v:
Biotechnology Techniques. 7:287-292
The incorporation of small glass hollow spheres into Ca-alginate beads allows the density of immobilized cell preparations to be decreased. For immobilized yeast, glass hollow spheres at 14% (v/v) decrease the density from 1088 to 996 kg m-3, thus gr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d75bb151cfbcd5961ec16d986c393fd2
https://doi.org/10.1007/bf00150900
https://doi.org/10.1007/bf00150900
Autor:
Ursula Rinas, David Estapé
Publikováno v:
Scopus-Elsevier
The efficiency of purification of basic fibroblast growth factor (bFGF) inclusion bodies using EDTA and nonionic detergents was improved from 25 to 40% by shifting the pH from 8.5 to strong alkaline conditions (pH 9.5 – 10.5). Complete dissolution
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5899fd97ab209468122c19c4c262fa34
http://www.scopus.com/inward/record.url?eid=2-s2.0-3542997762&partnerID=MN8TOARS
http://www.scopus.com/inward/record.url?eid=2-s2.0-3542997762&partnerID=MN8TOARS