Zobrazeno 1 - 10
of 13
pro vyhledávání: '"David E. Portwood"'
Autor:
James Hough, John D. Howard, Stephen Brown, David E. Portwood, Peter M. Kilby, Mark J. Dickman
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 10 (2022)
Current crop pest control strategies rely on insecticidal and fungicidal sprays, plant genetic resistance, transgenes and agricultural practices. However, many insects, plant viruses, and fungi have no current means of control or have developed resis
Externí odkaz:
https://doaj.org/article/a3eccdba05404b3b839e2cace1a41570
Autor:
Sandip Kumar, Mark J. Dickman, Peter M. Kilby, Alison O. Nwokeoji, David E. Portwood, Jamie K. Hobbs
Publikováno v:
The Analyst. 144:4985-4994
Long double-stranded (ds) RNA is emerging as a novel alternative to chemical and genetically-modified insect and fungal management strategies. The ability to produce large quantities of dsRNA in either bacterial systems, by in vitro transcription, in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c060b204fdd06bd39f4e1e86f9c51ba5
https://doi.org/10.1039/c9an00954j
https://doi.org/10.1039/c9an00954j
Publikováno v:
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
The emergence of new sustainable approaches for insect management using RNA interference (RNAi) based insecticides has created the demand for high throughput analytical techniques to fully characterise and accurately quantify double stranded RNA (dsR
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d61b983f1494cb58c683de148c21288e
https://doi.org/10.1016/j.jchromb.2018.11.027
https://doi.org/10.1016/j.jchromb.2018.11.027
Publikováno v:
Rapid Communications in Mass Spectrometry. 32:590-596
RATIONALE: Recent developments in RNA interference (RNAi) have created a need for cost-effective and large scale synthesis of double stranded RNA (dsRNA), in conjunction with high throughput analytical techniques to fully characterise and accurately
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35e83a31e070906dd4b9b627e9db50e0
https://doi.org/10.1002/rcm.8074
https://doi.org/10.1002/rcm.8074
Publikováno v:
Analytical Biochemistry. 512:36-46
RNASwift is an inexpensive, versatile method for the rapid extraction of RNA. Existing RNA extraction methods typically use hazardous chemicals including phenol, chloroform and formamide which are often difficult to completely remove from the extract
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::08651bb62de5798df5b109027599e3df
https://doi.org/10.1016/j.ab.2016.08.001
https://doi.org/10.1016/j.ab.2016.08.001
Publikováno v:
Analytical chemistry. 89(24)
UV absorbance spectrophotometry is widely used for the quantification of nucleic acids. For accurate quantification, it is important to determine the hypochromicity of the oligonucleotide or complex nucleic acid structure. The use of thermal denatura
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::419655d9a916e55f940ab1eb69bd41cd
https://pubmed.ncbi.nlm.nih.gov/29141408
https://pubmed.ncbi.nlm.nih.gov/29141408
Publikováno v:
Molecular Informatics
Liquid chromatography coupled to mass spectrometry (LC-MS) is commonly applied to investigate the small molecule complement of organisms. Several software tools are typically joined in custom pipelines to semi-automatically process and analyse the re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c657875a7ae57dd824eac680dd46360f
https://doi.org/10.1002/minf.201400016
https://doi.org/10.1002/minf.201400016
Autor:
Alison O, Nwokeoji, Sandip, Kumar, Peter M, Kilby, David E, Portwood, Jamie K, Hobbs, Mark J, Dickman
Publikováno v:
The Analyst. 144:6773-6773
Correction for ‘Analysis of long dsRNA produced in vitro and in vivo using atomic force microscopy in conjunction with ion-pair reverse-phase HPLC’ by Alison O. Nwokeoji, et al., Analyst, 2019, 144, 4985–4994.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24aa5a57510009b4ef2da4cfd2c23f5d
https://doi.org/10.1039/c9an90100k
https://doi.org/10.1039/c9an90100k
Autor:
Reza M. Salek, Charlie Hodgman, Graham B. Seymour, Charles Baxter, Stephan Beisken, David E. Portwood, Zsuzsanna Ament, Christoph Steinbeck, Rebecca Smith, Paul D. Fraser, Aniko Kende, Mark Seymour, Mark Earll
Publikováno v:
Scientific Data
Application of mass spectrometry enables the detection of metabolic differences between groups of related organisms. Differences in the metabolic fingerprints of wild-type Solanum lycopersicum and three monogenic mutants, ripening inhibitor (rin), no
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f9b2ac8d1e1c79ca1fcafbd082a0686
https://doi.org/10.1038/sdata.2014.29
https://doi.org/10.1038/sdata.2014.29
Autor:
David E. Portwood, Kerry A. Hale
Publikováno v:
Journal of Environmental Science and Health, Part B. 31:477-484
Spinosad is a natural product with biological activity against a range of insects including lepidoptera. It is comprised of two major components namely spinosyns A and D. The degradation of spinosad in soil under aerobic conditions was investigated u
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fff87d47a6cad0ca911f97265782e368
https://doi.org/10.1080/03601239609373010
https://doi.org/10.1080/03601239609373010