Zobrazeno 1 - 10
of 66
pro vyhledávání: '"David E, Wolf"'
Publikováno v:
Sensors, Vol 15, Iss 8, Pp 19709-19722 (2015)
Diffuse Correlation Spectroscopy (DCS) is a well-established optical technique that has been used for non-invasive measurement of blood flow in tissues. Instrumentation for DCS includes a correlation device that computes the temporal intensity autoco
Externí odkaz:
https://doaj.org/article/bb6427744da540eb8029930240866762
Autor:
David E. Wolf
Publikováno v:
Spectroscopic Membrane Probes ISBN: 9781351076807
Spectroscopic Membrane Probes
Spectroscopic Membrane Probes
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5c32fa6894402ba2d7babc66eb5c2bda
https://doi.org/10.1201/9781351076807-8
https://doi.org/10.1201/9781351076807-8
Autor:
David E. Wolf, Christine Thompson, Beth Brown, Zhenning Hong, Rama Bansil, Nezam H. Afdhal, Xingxiang Cao
Publikováno v:
Biomacromolecules. 5:269-275
We utilized fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spectroscopy (FCS) to examine the role of gallbladder mucin (GBM) in promoting the aggregation and/or fusion of cholesterol enriched vesicles. By fluorescent l
Autor:
Greenfield Sluder, David E. Wolf
The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital c
Publikováno v:
Proceedings of the National Academy of Sciences. 95:6043-6048
Fluorescein-labeled oligodeoxynucleotides (oligos) were introduced into cultured rat myoblasts, and their molecular movements inside the nucleus were studied by fluorescence correlation spectroscopy (FCS) and fluorescence recovery after photobleachin
Publikováno v:
Molecular Reproduction and Development. 50:86-92
The technique of fluorescence recovery after photobleaching (FRAP) was employed on spermatozoa labeled with the fluorescent lipid analogue C 14 dil to provide two measures of lateral diffusion in the plane of the sperm plasma membrane during capacita
Mobility of TrkA Is Regulated by Phosphorylation and Interactions with the Low-Affinity NGF Receptor
Autor:
David E. Wolf, Alonzo H. Ross, Marie-Claire Daou, Christine McKinnon-Thompson, David R. Kaplan, Robert M. Stephens
Publikováno v:
Biochemistry. 37:3178-3186
The nerve growth factor (NGF) receptor is a complex of two proteins, gp75 and the tyrosine kinase TrkA. Using fluorescence recovery after photobleaching, we have studied the diffusion properties of the TrkA receptor. For PC12 cells that express both
Autor:
David E, Wolf
Publikováno v:
Methods in cell biology. 114
Although geometric optics gives a good understanding of how the microscope works, it fails in one critical area, which is explaining the origin of microscope resolution. To accomplish this, one must consider the microscope from the viewpoint of physi
Autor:
David E, Wolf
Publikováno v:
Methods in cell biology. 114
This chapter discusses the fundamental physics of fluorescence. The application of fluorescence to microscopy represents an important transition in the development of microscopy, particularly as it applies to biology. It enables quantitating the amou
Publikováno v:
Methods in cell biology. 114
This chapter discusses quantitative analysis of digital microscope images and presents several exercises to provide examples to explain the concept. This chapter also presents the basic concepts in quantitative analysis for imaging, but these concept