A tale of two secondary structure elements: when a β-hairpin becomes an α-helix 1 1Edited by A. R. Fersht

Autor: Maria J. Macias, Gerlind Wallon, Luis Serrano, Concepción Civera, David Cregut

Publikováno v: Journal of Molecular Biology. 292:389-401

In this work, we have analyzed the relative importance of secondary versus tertiary interactions in stabilizing and guiding protein folding. For this purpose, we have designed four different mutants to replace the α-helix of the GB1 domain by a sequ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1e070cea770ba605822bfb67004818e0
https://doi.org/10.1006/jmbi.1999.2966

Site-directed mutagenesis of a calcium binding site modifies specifically the different biochemical properties of annexin I

Autor: Joannes Sri Widada, G. Travé, David Cregut, J.-F. Quignard, Corinne Lionne, Jean-Pierre Liautard, Laurent Chiche

Publikováno v: Protein Engineering, Design and Selection
Protein Engineering, Design and Selection, Oxford University Press (OUP), 1994, 7 (5), pp.689-696. ⟨10.1093/protein/7.5.689⟩

All the functions of annexins in vitro as well as in vivo are mediated and probably regulated by calcium. We have used recombinant annexin I, synthesized by Escherichia coli, and we have performed site-directed mutagenesis. We have mutated the endone

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49981ecd76007d5928a5e8ebc2958d82
https://doi.org/10.1093/protein/7.5.689

Statistical potentials and learning methods to evaluate protein models: application to the coiled-coil tropomyosin

Autor: Laurent Chiche, David Cregut, Jérôme Gracy

Publikováno v: Journal of Molecular Structure: THEOCHEM. 286:47-54

A machine learning system trained on mostly α-helical proteins was used to predict the helical propensity along six tropomyosin sequences. The average prediction profile showed flat plateaux separated by minima that appeared about every 40 residues

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e85d789e0aea638705889a08046ccf2b
https://doi.org/10.1016/0166-1280(93)87150-c

Towards the design and computational characterization of a membrane protein

Autor: D.P Tieleman, David Cregut, H.J.C. Berendsen, C.T. Choma, L. Serrano

Publikováno v: Journal of Molecular Graphics & Modelling, 20(3), 219-234

The design of a transmembrane four-helix bundle is described. We start with an idealized four-helix bundle geometry, then use statistical information to build a plausible transmembrane bundle. Appropriate residues are chosen using database knowledge

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6552266ce4ccd14b74e5d7ffb4da4844
https://pubmed.ncbi.nlm.nih.gov/11766047

Hinge-bending motions in annexins: molecular dynamics and essential dynamics of apo-annexin V and of calcium bound annexin V and I

Autor: David Cregut, Jean-Pierre Liautard, Guillaume Drin, Laurent Chiche

Publikováno v: Scopus-Elsevier

Annexins are homologous proteins that bind to membranes in a calcium dependent manner, but for which precise physiological roles have yet to be defined. Most annexins are composed of a planar array of four homologous repeats, each containing five alp

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a7000c74205e9e779c953774d7cc7aa
https://pubmed.ncbi.nlm.nih.gov/9862208