Zobrazeno 1 - 10
of 104
pro vyhledávání: '"David Baddeley"'
Publikováno v:
Frontiers in Molecular Biosciences, Vol 8 (2021)
The fusion pore is the initial narrow connection that forms between fusing membranes. During vesicular release of hormones or neurotransmitters, the nanometer-sized fusion pore may open-close repeatedly (flicker) before resealing or dilating irrevers
Externí odkaz:
https://doaj.org/article/01fcd20291524125bb67f74978da266e
Autor:
Yufeng Hou, Jizhong Bai, Xin Shen, Oscar de Langen, Amy Li, Sean Lal, Cristobal G. dos Remedios, David Baddeley, Peter N. Ruygrok, Christian Soeller, David J. Crossman
Publikováno v:
Frontiers in Physiology, Vol 12 (2021)
The disrupted organisation of the ryanodine receptors (RyR) and junctophilin (JPH) is thought to underpin the transverse tubule (t-tubule) remodelling in a failing heart. Here, we assessed the nanoscale organisation of these two key proteins in the f
Externí odkaz:
https://doaj.org/article/b2bbfa1206e548a38c1020f6a2d5211e
Publikováno v:
eLife, Vol 8 (2019)
Actin dynamics generate forces to deform the membrane and overcome the cell’s high turgor pressure during clathrin-mediated endocytosis (CME) in yeast, but precise molecular details are still unresolved. Our previous models predicted that actin fil
Externí odkaz:
https://doaj.org/article/40e9d46d3e734499b901a70f919e5855
Autor:
Isuru Jayasinghe, Alexander H. Clowsley, Ruisheng Lin, Tobias Lutz, Carl Harrison, Ellen Green, David Baddeley, Lorenzo Di Michele, Christian Soeller
Publikováno v:
Cell Reports, Vol 22, Iss 2, Pp 557-567 (2018)
Summary: Signaling nanodomains rely on spatial organization of proteins to allow controlled intracellular signaling. Examples include calcium release sites of cardiomyocytes where ryanodine receptors (RyRs) are clustered with their molecular partners
Externí odkaz:
https://doaj.org/article/16126721d7004c8086a4dbcafdb5274d
Spontaneous Activity Patterns Are Altered in the Developing Visual Cortex of the Fmr1 Knockout Mouse
Publikováno v:
Frontiers in Neural Circuits, Vol 13 (2019)
Fragile X syndrome (FXS) is the most prevalent inherited cause of autism and is accompanied by behavioral and sensory deficits. Errors in the wiring of the brain during early development likely contribute to these deficits, but the underlying mechani
Externí odkaz:
https://doaj.org/article/c365670191074ad99966392c5f440c7a
Autor:
Francesca Bottanelli, Emil B. Kromann, Edward S. Allgeyer, Roman S. Erdmann, Stephanie Wood Baguley, George Sirinakis, Alanna Schepartz, David Baddeley, Derek K. Toomre, James E. Rothman, Joerg Bewersdorf
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-5 (2016)
The intracellular applications of STED microscopy are limited by the availability of dyes. Here the authors develop a two-colour labelling strategy based on SiR and ATTO590 dyes, and apply their strategy to image various subcellular membrane compartm
Externí odkaz:
https://doaj.org/article/ea973638e80347418c454d3d0a47cc6b
Autor:
David Baddeley, David Crossman, Sabrina Rossberger, Juliette E Cheyne, Johanna M Montgomery, Isuru D Jayasinghe, Christoph Cremer, Mark B Cannell, Christian Soeller
Publikováno v:
PLoS ONE, Vol 6, Iss 5, p e20645 (2011)
BackgroundOptical super-resolution imaging of fluorescently stained biological samples is rapidly becoming an important tool to investigate protein distribution at the molecular scale. It is therefore important to develop practical super-resolution m
Externí odkaz:
https://doaj.org/article/275e5a23c994488d9b10d4bad1c78623
Autor:
Andrew E S Barentine, Yu Lin, Edward M Courvan, Phylicia Kidd, Miao Liu, Leonhard Balduf, Timy Phan, Felix Rivera-Molina, Michael R Grace, Zach Marin, Mark Lessard, Juliana Rios Chen, Siyuan Wang, Karla M Neugebauer, Joerg Bewersdorf, David Baddeley
Publikováno v:
Nature Biotechnology.
Diffraction-unlimited single-molecule techniques like STORM and (F)PALM enable three-dimensional (3D) fluorescence imaging at tens of nanometer resolution and are invaluable to investigate sub-cellular organization. The multitude of camera frames req
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6cdd0517d4be9044eaab9ffc37a50ce5
https://doi.org/10.1038/s41587-023-01702-1
https://doi.org/10.1038/s41587-023-01702-1
Autor:
Kenny K. H. Chung, Zhao Zhang, Phylicia Kidd, Yongdeng Zhang, Nathan D. Williams, Bennett Rollins, Yang Yang, Chenxiang Lin, David Baddeley, Joerg Bewersdorf
Publikováno v:
Nature Methods. 19:554-559
DNA-based points accumulation for imaging in nanoscale topography (DNA-PAINT) is a powerful super-resolution microscopy method that can acquire high-fidelity images at nanometer resolution. It suffers, however, from high background and slow imaging s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a71dbc882047a8e99b75b661c1c54233
https://doi.org/10.1038/s41592-022-01464-9
https://doi.org/10.1038/s41592-022-01464-9
Autor:
David Benoist, David Baddeley, Nicolas P. Smith, Richard D. Walton, Gregory B. Sands, Bruce H. Smaill, Olivier Bernus, Igor R. Efimov, Mark L. Trew, Jesse L. Ashton
Publikováno v:
Progress in Biophysics and Molecular Biology. 168:18-32
Recent developments in clearing and microscopy enable 3D imaging with cellular resolution up to the whole organ level. These methods have been used extensively in neurobiology, but their uptake in other fields has been much more limited. Application
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51aa8aaa504638ea631c9ebf9307c56e
https://doi.org/10.1016/j.pbiomolbio.2021.06.005
https://doi.org/10.1016/j.pbiomolbio.2021.06.005