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pro vyhledávání: '"David B. Wilde"'
Publikováno v:
ImmunoMethods. 1:177-183
The polymerase chain reaction is a powerful tool for deriving semi-quantitative information about the relative abundance of cytokine RNA species that are present in pathologic states, such as autoimmune disease. We describe here the MAPPing technique
Autor:
Ethan M. Shevach, R Valas, Knut Sturmhöfel, David B. Wilde, Angel Ezquerra, John E. Coligan, Thomas J. McConnell
Publikováno v:
European Journal of Immunology. 22:491-498
A panel of dendritic epidermal T cell (DETC) lines, and hybridomas derived from them, has been shown to spontaneously secrete lymphokines in the absence of added stimuli, which suggests that these cells are autoreactive. These cell lines are characte
Autor:
Kewan Roberts, David B. Wilde, Gary Kikuchi, Knut Sturmhöfel, John E. Coligan, Ethan M. Shevach
Publikováno v:
European journal of immunology. 22(2)
A minor population of dendritic epidermal T cells (DETC) express the V gamma 1.1C gamma 4V delta 6 T cell receptor and T cell clones and hybridomas derived from this subset constitutively secrete cytokines in culture secondary to recognition of an au