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Akademický článek

Abstract OR-16: High Resolution Cryo-Electron Microscopy and New Direct Electron Detector from Gatan - K3

Autor: Alexander Myasnikov, Shawn Zheng, David Bulkley, Yifan Cheng, David Agard

Publikováno v: International Journal of Biomedicine, Vol 9, Iss Suppl_1, Pp S12-S12 (2019)

Background: Much of the success of modern cryo-Electron microscopy is the result of advances in electron detector, specifically the advent of direct electron detectors [Kuhlbrandt, 2014, Biochemistry]. Of particular utility has been the Gatan K2 came

Externí odkaz: https://doaj.org/article/d7d4650ef20e417296b1a14ac0844f84

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Akademický článek

Hsp70 Forms Antiparallel Dimers Stabilized by Post-translational Modifications to Position Clients for Transfer to Hsp90

Autor: Nina Morgner, Carla Schmidt, Victoria Beilsten-Edmands, Ima-obong Ebong, Nisha A. Patel, Eugenia M. Clerico, Elaine Kirschke, Soumya Daturpalli, Sophie E. Jackson, David Agard, Carol V. Robinson

Publikováno v: Cell Reports, Vol 11, Iss 5, Pp 759-769 (2015)

Protein folding in cells is regulated by networks of chaperones, including the heat shock protein 70 (Hsp70) system, which consists of the Hsp40 cochaperone and a nucleotide exchange factor. Hsp40 mediates complex formation between Hsp70 and client p

Externí odkaz: https://doaj.org/article/44cbb207b5e1483da5af592660e5f1ce

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Akademický článek

Protein Expression and Purification of the Hsp90-Cdc37-Cdk4 Kinase Complex from Saccharomyces cerevisiae

Autor: Kliment Verba, David Agard

Publikováno v: Bio-Protocol, Vol 7, Iss 19 (2017)

Interactions between Hsp90, its co-chaperone Cdc37 and kinases have been biochemically studied for over three decades and have been shown to be functionally important in organisms from yeast to humans. However, formation of a stable complex for struc

Externí odkaz: https://doaj.org/article/b577c9a007a240d08f3b0a05103da42d

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Akademický článek

Condensed mitotic chromosome structure at nanometer resolution using PALM and EGFP- histones.

Autor: Atsushi Matsuda, Lin Shao, Jerome Boulanger, Charles Kervrann, Peter M Carlton, Peter Kner, David Agard, John W Sedat

Publikováno v: PLoS ONE, Vol 5, Iss 9, p e12768 (2010)

Photoactivated localization microscopy (PALM) and related fluorescent biological imaging methods are capable of providing very high spatial resolutions (up to 20 nm). Two major demands limit its widespread use on biological samples: requirements for

Externí odkaz: https://doaj.org/article/e715c3be77f44c93838d16555c75ea58

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Structural features of mTORC2 that control substrate-specific activities

Autor: Zanlin Yu, Junliang Chen, Enzo Takagi, Feng Wang, Bidisha Saha, Xi Liu, Lydia-Marie Joubert, Catherine E. Gleason, Mingliang Jin, Chengmin Li, Carlos Nowotny, David Agard, Yifan Cheng, David Pearce

mTORC2 is a multi-subunit kinase complex that is central to multiple essential signaling pathways. Two core subunits, Rictor and mSin1 distinguish it from its relative, mTORC1 and support context-dependent phosphorylation of its substrates. mTORC2 st

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::55c72a063ae12142da801ad1db253226
https://doi.org/10.1101/2022.02.03.478898

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Entropy-regularized deconvolution of cellular cryotransmission electron tomograms

Autor: Matthew Croxford, Michael Elbaum, Muthuvel Arigovindan, Zvi Kam, David Agard, Elizabeth Villa, John Sedat

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, vol 118, iss 50

Significance Cellular cryo-electron tomography suffers from severely compromised Z resolution due to the missing wedges of information not collected during the acquisition of tilt series. This paper shows that application of entropy-regularized decon

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b35bc87268a7a480f36068e6410ec175
http://europepmc.org/articles/PMC8685678

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Interactions between mTORC2 core subunits Rictor and mSin1 dictate selective and context-dependent phosphorylation of substrate kinases SGK1 and Akt

Publikováno v: The Journal of biological chemistry, vol 298, iss 9

Mechanistic target of rapamycin complex 2 (mTORC2) is a multi-subunit kinase complex, central to multiple essential signaling pathways. Two core subunits, Rictor and mSin1, distinguish it from the related mTORC1 and support context-dependent phosphor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18a54ee2ce88a9c9c1c8646842e1df3f
https://doi.org/10.1016/j.jbc.2022.102288

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[The basics of immobilisation]

Autor: Isabelle, Rebours, David, Agard, Cécile, Messager, Christelle, Cattelin, Olivier, Barbier, Camille, Choufani

Publikováno v: Revue de l'infirmiere. 70(267)

Treatments for a patient with severe limb trauma, require immobilisation in order to stabilise and thus allow bone consolidation or ligament healing or skin protection.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::7e0bf53fce7bb91862b3445f3f30c673
https://pubmed.ncbi.nlm.nih.gov/33455677

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