CRISPR/Cas9-Targeted Mutagenesis of CiGAS and CiGAO to Reduce Bitterness in Chicory (Cichorium intybus L.)

Autor: Justine Domont, Margot Thiblet, Audrey Etienne, Harmony Alves Dos Santos, Thierry Cadalen, Philippe Hance, David Gagneul, Jean-Louis Hilbert, Caroline Rambaud

Publikováno v: Frontiers in Bioscience-Landmark, Vol 28, Iss 9, p 201 (2023)

Background: Chicory (Cichorium intybus L.), a member of the Asteraceae family, is known for its numerous health benefits, including its prebiotic, digestive, antioxidant or anti-inflammatory effects. Used as a coffee substitute, chicory roots is also

Externí odkaz: https://doaj.org/article/79d4e88ff4884ad082865ce0482c836c

MeJA Elicitation of Chicory Hairy Roots Promotes Efficient Increase of 3,5-diCQA Accumulation, a Potent Antioxidant and Antibacterial Molecule

Autor: Guillaume Bernard, Harmony Alves Dos Santos, Audrey Etienne, Jennifer Samaillie, Christel Neut, Sevser Sahpaz, Jean-Louis Hilbert, David Gagneul, Nathalie Jullian, Ali Tahrioui, Sylvie Chevalier, Céline Rivière, Caroline Rambaud

Publikováno v: Antibiotics, Vol 9, Iss 10, p 659 (2020)

Cichorium intybus L. (Asteraceae) is an important industrial crop, as well as a medicinal plant which produces some bioactive compounds implicated in various biological effects with potential applications in human health. Particularly, roots produce

Externí odkaz: https://doaj.org/article/6c1073164a9e45b9b732b22127e3ce04

Efficient Genome Editing Using CRISPR/Cas9 Technology in Chicory

Autor: Guillaume Bernard, David Gagneul, Harmony Alves Dos Santos, Audrey Etienne, Jean-Louis Hilbert, Caroline Rambaud

Publikováno v: International Journal of Molecular Sciences, Vol 20, Iss 5, p 1155 (2019)

CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated with protein CAS9) is a genome-editing tool that has been extensively used in the last five years because of its novelty, affordability, and feasibility. This te

Externí odkaz: https://doaj.org/article/a3a062dc2dd9472395631470bc511706

Exploiting Spermidine N -Hydroxycinnamoyltransferase Diversity and Substrate Promiscuity to Produce Various Trihydroxycinnamoyl Spermidines and Analogues in Engineered Yeast

Autor: Thibaut Munsch, Natalja Kulagina, David Gagneul, Marianne Unlubayir, Benoit St-Pierre, Arnaud Lanoue, Thomas Dugé de Bernonville, Vincent Courdavault, Inês Carqueijeiro, Nathalie Giglioli-Guivarc’h, Johan-Owen De Craene, Jennifer Perrin, Marc Clastre, Sébastien Besseau

Publikováno v: ACS Synthetic Biology
ACS Synthetic Biology, American Chemical Society, 2021, 10 (2), pp.286-296. ⟨10.1021/acssynbio.0c00391⟩
ACS Synthetic Biology, 2021, 10 (2), pp.286-296. ⟨10.1021/acssynbio.0c00391⟩

International audience; Trihydroxycinnamoyl spermidines (THCSpd) are plant specialized metabolites with promising pharmacological activities as antifungals, antibacterial, antiviral, and antidepressant drugs. However, their characterization and poten

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c1c2ddc060fa278825decfd4c165cf9
https://hal-univ-tours.archives-ouvertes.fr/hal-03287505

Phenolamides in plants: An update on their function, regulation, and origin of their biosynthetic enzymes

Autor: David Gagneul, Marwa Roumani, Sébastien Besseau, Christophe Robin, Romain Larbat

Publikováno v: Journal of Experimental Botany
Journal of Experimental Botany, Oxford University Press (OUP), 2021, 72 (7), pp.2334-2355. ⟨10.1093/jxb/eraa582⟩
Journal of Experimental Botany, 2021, 72 (7), pp.2334-2355. ⟨10.1093/jxb/eraa582⟩

Phenolamides represent a family of specialized metabolites, consisting of the association of hydroxycinnamic acid derivatives with aliphatic or aromatic amines. Since the discovery of the first phenolamide in the late 1940s, decades of phytochemical

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d28e9976857474456bd8a4f271d5e248
https://hal.univ-lorraine.fr/hal-03066081/document