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Akademický článek

NegFluo, a Fast and Efficient Method to Determine Starch Granule Size and Morphology In Situ in Plant Chloroplasts

Autor: Camille Vandromme, Angelina Kasprowicz, Adeline Courseaux, Dave Trinel, Maud Facon, Jean-Luc Putaux, Christophe D’Hulst, Fabrice Wattebled, Corentin Spriet

Publikováno v: Frontiers in Plant Science, Vol 10 (2019)

Starch granules that accumulate in the plastids of plants vary in size, shape, phosphate, or protein content according to their botanical origin. Depending on their size, the applications in food and nonfood industries differ. Being able to master st

Externí odkaz: https://doaj.org/article/cc13d9ac30f443e0a1460333d813d670

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Akademický článek

Optimization of ERK Activity Biosensors for both Ratiometric and Lifetime FRET Measurements

Autor: Pauline Vandame, Corentin Spriet, Franck Riquet, Dave Trinel, Katia Cailliau-Maggio, Jean-François Bodart

Publikováno v: Sensors, Vol 14, Iss 1, Pp 1140-1154 (2014)

Among biosensors, genetically-encoded FRET-based biosensors are widely used to localize and measure enzymatic activities. Kinases activities are of particular interest as their spatiotemporal regulation has become crucial for the deep understanding o

Externí odkaz: https://doaj.org/article/4a95a607a6e5488e9303f8905dcba98e

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Akademický článek

NS2 protein of hepatitis C virus interacts with structural and non-structural proteins towards virus assembly.

Autor: Costin-Ioan Popescu, Nathalie Callens, Dave Trinel, Philippe Roingeard, Darius Moradpour, Véronique Descamps, Gilles Duverlie, François Penin, Laurent Héliot, Yves Rouillé, Jean Dubuisson

Publikováno v: PLoS Pathogens, Vol 7, Iss 2, p e1001278 (2011)

Growing experimental evidence indicates that, in addition to the physical virion components, the non-structural proteins of hepatitis C virus (HCV) are intimately involved in orchestrating morphogenesis. Since it is dispensable for HCV RNA replicatio

Externí odkaz: https://doaj.org/article/aac1d161aed24e28862061d99b3924ef

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FRET-Based Enzyme Activity Reporter: Practical Hints for Kinases as Indicators of Virulence

Autor: Dave Trinel, Jean-François Bodart, Corentin Spriet, Angelina Kasprowicz

Publikováno v: Biosensing Technologies for the Detection of Pathogens-A Prospective Way for Rapid Analysis
Biosensing Technologies for the Detection of Pathogens-A Prospective Way for Rapid Analysis, InTech, 2018, 978-953-51-3916-4. ⟨10.5772/intechopen.72388⟩

International audience; Modulation of protein kinases activity is often requested for pathogenicity or virulence. This chapter provides several hints for one who might be interested in using FRET-based kinase activity reporters. The archetypes of the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c246924bfa25dec40543e89ece7bdfdf
https://hal.univ-lille.fr/hal-03007678/document

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High-capacity data hiding in encrypted images using MSB prediction

Autor: Dave Trinel, Pauline Puteaux, William Puech

Publikováno v: IPTA
6th International Conference on Image Processing Theory Tools and Applications
IPTA: Image Processing Theory Tools and Applications
IPTA: Image Processing Theory Tools and Applications, Dec 2016, Oulu, Finland. ⟨10.1109/IPTA.2016.7820991⟩

International audience; In the last few years, visual privacy has become a major problem. Because of this, encrypted image processing has received a lot of attention within the scientific and business communities. Data hiding in encrypted images (DHE

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fd86bf5e0620b7bec8251a646cee266
https://doi.org/10.1109/ipta.2016.7820991

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Biometric data assessment on Xenopus laevis tadpoles

Autor: Jean-François Bodart, Dave Trinel, Corentin Spriet, Sylvain Slaby, Sébastien Lemière, Matthieu Marin, Julie Hanotel, Alain Leprêtre

Publikováno v: Journal of Xenobiotics
Journal of Xenobiotics, Page Press publications, 2016, 6 (2), pp.33-35. ⟨10.4081/xeno.2016.6587⟩
Journal of Xenobiotics, 2016, 6 (2), pp.33-35. ⟨10.4081/xeno.2016.6587⟩
Journal of Xenobiotics, Vol 6, Iss 2 (2016)
Journal of Xenobiotics; Volume 6; Issue 2; Pages: 6587
Journal of Xenobiotics 2 (6), 33-35. (2016)

Recent studies stated that 41% of known species of amphibians are threatened.1 Indeed, their dramatic population decline is due to environmental conditions, including pollution.2,3 Their permeable skin and their close relationship with aquatic compar

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::934430983667bb185115e83c872cba9e
https://hal.archives-ouvertes.fr/hal-01606980

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Generalization of the polar representation in time domain fluorescence lifetime imaging microscopy for biological applications: practical implementation

Autor: Aymeric Leray, Corentin Spriet, Laurent Héliot, Dave Trinel, Yves Usson

Publikováno v: Journal of Microscopy. 248:66-76

Summary The polar representation or phasor, which provides a fast and visual indication on the number of exponentials present in the intensity decay of the fluorescence lifetime images is increasingly used in time domain fluorescence lifetime imaging

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2cd5ba112d31499808bc852dffa42799
https://doi.org/10.1111/j.1365-2818.2012.03651.x

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Upgrading time domain FLIM using an adaptive Monte Carlo data inflation algorithm

Autor: Yves Usson, Corentin Spriet, Aymeric Leray, Dave Trinel, Laurent Héliot

Publikováno v: Cytometry Part A. :528-537

Fluorescence Lifetime Imaging Microscopy (FLIM) is a powerful technique to investigate the local environment of fluorophores in living cells. To correctly estimate all lifetime parameters, time domain FLIM imaging requires a high number of photons an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8cf413c5387798acd5750b745e39be8e
https://doi.org/10.1002/cyto.a.21054

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Quantitative comparison of polar approach versus fitting method in time domain FLIM image analysis

Autor: Corentin Spriet, Aymeric Leray, Dave Trinel, Yves Usson, Ralf Blossey, Laurent Héliot

Publikováno v: Cytometry Part A
Cytometry Part A, 2011, 79 (2), pp.149-58. ⟨10.1002/cyto.a.20996⟩
Cytometry Part A, Wiley, 2011, 79 (2), pp.149-58. ⟨10.1002/cyto.a.20996⟩

International audience; We calculate here analytically the performance of the polar approach (or phasor) in terms of signal-to-noise ratio and F values when performing time-domain Fluorescence Lifetime Imaging Microscopy (FLIM) to determine the minim

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4eefd89f61f17fb2ee68c6e06dd5f83c
https://doi.org/10.1002/cyto.a.20996

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Three-dimensional polar representation for multispectral fluorescence lifetime imaging microscopy

Autor: Corentin Spriet, Dave Trinel, Laurent Héliot, Aymeric Leray

Publikováno v: Cytometry Part A. :1007-1014

Multispectral fluorescence lifetime imaging microscopy is a promising and powerful technique for discriminating multiply labeled samples and for detecting molecular interactions inside thick, heterogeneous, and light-scattering milieu such as tissue.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::556a5c263b320d418b07cf3bea10b9e7
https://doi.org/10.1002/cyto.a.20802

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