Single-molecule force spectroscopy reveals the dynamic strength of the hair-cell tip-link connection

Autor: Eric M. Mulhall, Andrew Ward, Darren Yang, Mounir A. Koussa, David P. Corey, Wesley P. Wong

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-15 (2021)

The conversion of auditory and vestibular stimuli into electrical signals is initiated by force transmitted to a mechanotransduction channel through the tip link. Here authors show that a single tip-link bond is more mechanically stable relative to c

Externí odkaz: https://doaj.org/article/cedc8ced7e7c4b7984736d122c8d046c

Flow-induced elongation of von Willebrand factor precedes tension-dependent activation

Autor: Hongxia Fu, Yan Jiang, Darren Yang, Friedrich Scheiflinger, Wesley P. Wong, Timothy A. Springer

Publikováno v: Nature Communications, Vol 8, Iss 1, Pp 1-12 (2017)

Von Willebrand factor (VWF) is a blood protein involved in clotting and is proposed to be activated by flow, but the mechanism is unknown. Here the authors show that VWF is first converted from a compact to linear form by flow, and is subsequently ac

Externí odkaz: https://doaj.org/article/a5fbfb5781ac4911a873b2e2e5610c15

Resolving Molecular Heterogeneity with Single-Molecule Centrifugation

Autor: Yi Luo, Jeffrey Chang, Darren Yang, J. Shepard Bryan, Molly MacIsaac, Steve Pressé, Wesley P. Wong

Publikováno v: Journal of the American Chemical Society. 145:3276-3282

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::db2caada4105e48959b4edbf55ceca36
https://doi.org/10.1021/jacs.2c11450

Single-molecule mechanical fingerprinting with DNA nanoswitch calipers

Autor: Andrew Ward, Darren Yang, Wesley P. Wong, Serkan Cabi, Elisha Krieg, Bhavik Nathwani, Prakash Shrestha, Yi Luo, Toma E. Tomov, William M. Shih, James I. MacDonald, Hans T. Bergal, Alexander Johnson-Buck

Publikováno v: Nature Nanotechnology. 16:1362-1370

Decoding the identity of biomolecules from trace samples is a longstanding goal in the field of biotechnology. Advances in DNA analysis have substantially affected clinical practice and basic research, but corresponding developments for proteins face

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::726d7e5e758032a33e31cc8662ea38e0
https://doi.org/10.1038/s41565-021-00979-0

Stretching DNA to twice the normal length with single-molecule hydrodynamic trapping

Autor: Yan Jiang, Darren Yang, Wesley P. Wong, Julia A M Bakx, Theodore Feldman

Publikováno v: Lab Chip
Lab on a Chip, 20(10), 1780-1791. Royal Society of Chemistry
Jiang, Y, Feldman, T, Bakx, J A M, Yang, D & Wong, W P 2020, ' Stretching DNA to twice the normal length with single-molecule hydrodynamic trapping ', Lab on a Chip, vol. 20, no. 10, pp. 1780-1791 . https://doi.org/10.1039/c9lc01028a

Single-molecule force spectroscopy has brought many new insights into nanoscale biology, from the functioning of molecular motors, to the mechanical response of soft materials within the cell. Yet in the extreme-force regime, limitations in current a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec217f7ac4ee0a55c68ff2ae2709680b
https://europepmc.org/articles/PMC7239757/