Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Darren L. Beene"'
Autor:
Jamie A. Ashby, Dennis A. Dougherty, Neil J. Harrison, Darren L. Beene, Jinti Wang, Sarah C. R. Lummis, Katherine S. Millen
Publikováno v:
ACS Chemical Neuroscience
The ligand binding site of Cys-loop receptors is dominated by aromatic amino acids. In GABA(C) receptors, these are predominantly tyrosine residues, with a number of other aromatic residues located in or close to the binding pocket. Here we examine t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::422ca115ff1040018bde8e94063a2133
http://europepmc.org/articles/PMC3309607
http://europepmc.org/articles/PMC3309607
Autor:
John D. Scott, Darren L Beene
Publikováno v:
Current Opinion in Cell Biology. 19:192-198
A-kinase anchoring proteins (AKAPs) are signaling scaffolds that contribute to various aspects of cAMP signaling. They do this by tethering protein kinase-A to specific subcellular sites, thereby focusing its activity toward relevant substrates. Rece
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aedaf8244b92253792b5f36ec9996ac0
https://doi.org/10.1016/j.ceb.2007.02.011
https://doi.org/10.1016/j.ceb.2007.02.011
Publikováno v:
The Journal of Neuroscience. 24:9097-9104
The mechanism by which agonist binding triggers pore opening in ligand-gated ion channels is poorly understood. Here, we used unnatural amino acid mutagenesis to introduce subtle changes to the side chains of tyrosine residues (Tyr141, Tyr143, Tyr153
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db1bc4b4fa126ca78e96d061c005300f
https://doi.org/10.1523/jneurosci.2429-04.2004
https://doi.org/10.1523/jneurosci.2429-04.2004
Publikováno v:
Current Opinion in Neurobiology. 13:264-270
Unnatural amino acid mutagenesis makes possible the site-specific incorporation of synthetic amino acids, enabling detailed structure–function studies as well as the incorporation of biophysical probes. This method has been adapted for use with het
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6735afd7631f494dbfab4eb7cc06caeb
https://doi.org/10.1016/s0959-4388(03)00068-0
https://doi.org/10.1016/s0959-4388(03)00068-0
Autor:
Darren L. Beene, Henry A. Lester, R. William Broadhurst, Dennis A. Dougherty, Sarah C. R. Lummis, Lori W. Lee
5-Hydroxytryptamine type 3 (5-HT_3) receptors are members of the Cys-loop receptor superfamily. Neurotransmitter binding in these proteins triggers the opening (gating) of an ion channel by means of an as-yet-uncharacterized conformational change. He
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54946586e2795e0ed190d88c2e3bd1f6
https://resolver.caltech.edu/CaltechAUTHORS:20150319-085128410
https://resolver.caltech.edu/CaltechAUTHORS:20150319-085128410
Publikováno v:
Chemistrybiology. 12(9)
GABA(C) (rho) receptors are members of the Cys-loop superfamily of neurotransmitter receptors, which includes nicotinic acetylcholine (nACh), 5-HT(3), and glycine receptors. As in other members of this family, the agonist binding site of GABA(C) rece
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c0581ef37f13813ff91167e148bb898
https://pubmed.ncbi.nlm.nih.gov/16183023
https://pubmed.ncbi.nlm.nih.gov/16183023
Autor:
Gabriel S. Brandt, Wenge Zhong, Darren L. Beene, Henry A. Lester, Niki M. Zacharias, Dennis A. Dougherty
Publikováno v:
Biochemistry. 41(32)
A series of tryptophan analogues has been introduced into the binding site regions of two ion channels, the ligand-gated nicotinic acetylcholine and serotonin 5-HT(3A) receptors, using unnatural amino acid mutagenesis and heterologous expression in X
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6bd2cb70bc9ec590a7c34800fdf7d965
https://pubmed.ncbi.nlm.nih.gov/12162741
https://pubmed.ncbi.nlm.nih.gov/12162741