Zobrazeno 1 - 10
of 73
pro vyhledávání: '"Darrell P, Chandler"'
Autor:
Nitu Thakore, Ryan Norville, Molly Franke, Roger Calderon, Leonid Lecca, Michael Villanueva, Megan B Murray, Christopher G Cooney, Darrell P Chandler, Rebecca C Holmberg
Publikováno v:
PLoS ONE, Vol 13, Iss 7, p e0199869 (2018)
Automated nucleic acid extraction from primary (raw) sputum continues to be a significant technical challenge for molecular diagnostics. In this work, we developed a prototype open-architecture, automated nucleic acid workstation that includes a mech
Externí odkaz:
https://doaj.org/article/9a47a5771c654d108f3fa774fea990cd
Autor:
Christopher G. Cooney, Nicole Parrish, Megan Murray, Arial Bueno, Ryan Norville, Rebecca C. Holmberg, Alexander Kukhtin, Darrell P. Chandler, Peter Qu
Publikováno v:
Analytical Chemistry. 92:5311-5318
Automated genotyping of drug-resistant Mycobacterium tuberculosis (MTB) directly from sputum is challenging for three primary reasons. First, the sample matrix, sputum, is highly viscous and heterogeneous, posing a challenge for sample processing. Se
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d3c983425f1ee1828ca2e8b966bbf49e
https://doi.org/10.1021/acs.analchem.9b05853
https://doi.org/10.1021/acs.analchem.9b05853
Publikováno v:
BioTechniques, Vol 39, Iss 1, Pp 99-107 (2005)
Protein profiling and characterization of protein interactions in biological samples ultimately require indicator-free methods of signal detection, which likewise offer an opportunity to distinguish specific interactions from nonspecific protein bind
Externí odkaz:
https://doaj.org/article/6d81dcaa185c4996a04471eec624f1ca
Autor:
Darrell P. Chandler, Ann E. Jarrell
Publikováno v:
BioTechniques, Vol 38, Iss 4, Pp 591-600 (2005)
With the rapid pace of nucleic acid microarray technology development and a renewed national emphasis on detecting and characterizing microorganisms in environmental samples, there is a rush to operationalize existing microarray technologies and appl
Externí odkaz:
https://doaj.org/article/315e30b6fd6d426992dc02f5b6ae31a9
Publikováno v:
BioTechniques, Vol 30, Iss 2, Pp 368-379 (2001)
Microarrays printed on glass slides are often constructed by covalently linking oligonucleotide probes to a derivatized surface. These procedures typically require relatively expensive amine- or thiol-modified oligonucleotide probes that add consider
Externí odkaz:
https://doaj.org/article/2d62036994ad401f81bd1c522eb2297a
Autor:
Alexander V, Kukhtin, Ryan, Norville, Arial, Bueno, Peter, Qu, Nicole, Parrish, Megan, Murray, Darrell P, Chandler, Rebecca C, Holmberg, Christopher G, Cooney
Publikováno v:
Anal Chem
Automated genotyping of drug-resistant Mycobacterium tuberculosis (MTB) directly from sputum is challenging for three primary reasons. First, the sample matrix – sputum – is highly viscous and heterogeneous, posing a challenge for sample processi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::30a892e63e59194e0e9d520422c5745c
https://pubmed.ncbi.nlm.nih.gov/32142258
https://pubmed.ncbi.nlm.nih.gov/32142258
Autor:
Yvonne Linger, Rebecca C. Holmberg, Christopher G. Cooney, Alex Perov, Thomas Sebastian, Alexander C Kukhtin, Arial Bueno, Peter Qu, Julia Golova, Christopher Knickerbocker, Michael Villanueva, Darrell P. Chandler
Publikováno v:
Lab on a chip. 19(7)
We describe a Lab-on-a-Film disposable that detects multidrug-resistant tuberculosis (MDR-TB) from sputum extracts. The Lab-on-a-Film disposable consists of 203 gel elements that include DNA sequences (probes) for 37 mutations, deletions, or insertio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82fd3bdc6580aaf8a086fb5006538f67
https://pubmed.ncbi.nlm.nih.gov/30801596
https://pubmed.ncbi.nlm.nih.gov/30801596
Autor:
Rebecca C. Holmberg, Megan Murray, Molly F. Franke, Michael Villanueva, Ryan Norville, Christopher G. Cooney, Nitu Thakore, Darrell P. Chandler, Roger Calderon, Leonid Lecca
Publikováno v:
PLoS ONE
PLoS ONE, Vol 13, Iss 7, p e0199869 (2018)
PLoS ONE, Vol 13, Iss 7, p e0199869 (2018)
Automated nucleic acid extraction from primary (raw) sputum continues to be a significant technical challenge for molecular diagnostics. In this work, we developed a prototype open-architecture, automated nucleic acid workstation that includes a mech
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3de403dc1c121703fecc01e523eb2924
http://europepmc.org/articles/PMC6033430
http://europepmc.org/articles/PMC6033430
Autor:
Molly F. Franke, Nitu Thakore, Peter Qu, Megan Murray, Yvonne Linger, Rebecca C. Holmberg, Leonid Lecca, Roger Calderon, Christopher Knickerbocker, Alexander Kukhtin, Darrell P. Chandler, Julia Golova, David Sipes, Christopher G. Cooney
Publikováno v:
Journal of Clinical Microbiology
There is a growing awareness that molecular diagnostics for detect-to-treat applications will soon need a highly multiplexed mutation detection and identification capability. In this study, we converted an open-amplicon microarray hybridization test
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::668b5e0ea04f3e697420c1423aef6ecb
https://doi.org/10.1128/jcm.01652-17
https://doi.org/10.1128/jcm.01652-17
Autor:
Rebecca C. Holmberg, Peter Qu, Ryan Norville, Nitu Thakore, Steve Garber, Christopher G. Cooney, Arial Bueno, Darrell P. Chandler, Michael Villanueva
Publikováno v:
Journal of microbiological methods. 148
Systems that automate extraction of nucleic acid from cells or viruses in complex clinical matrices have tremendous value even in the absence of an integrated downstream detector. We describe our bench-top automated workstation that integrates our pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ecd741aaa126193dfb8a8f3dccbace5
https://pubmed.ncbi.nlm.nih.gov/31514020
https://pubmed.ncbi.nlm.nih.gov/31514020