Výsledky vyhledávání - "Darrell N. Ward"

Characterization of cleavage products in selected human lutropin preparations

Autor: Hyun S. Nahm, Darrell N. Ward, Ted Wen, Stephan D. Glenn

Publikováno v: International Journal of Peptide and Protein Research. 27:70-78

Low molecular weight fragments derived from the beta subunit of human lutropin have been frequently observed. These fragments are detected by polyacrylamide gel electrophoresis in sodium dodecyl sulfate following reduction of the disulfide bonds. A s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54b42820e2a4671c87300abd5354cf43
https://doi.org/10.1111/j.1399-3011.1986.tb02767.x

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Structural Analysis of N-Linked Oligosaccharides of Equine Chorionic Gonadotropin and Lutropin .beta.-Subunits

Autor: Koiti Titani, Hiromu Sugino, Tsuguo Mizuochi, Darrell N. Ward, George R. Bousfield, Taei Matsui, Motonori Hoshi, Tatsuyuki Okinaga

Publikováno v: Biochemistry. 33:14039-14048

Equine chorionic gonadotropin (eCG) and lutropin (eLH) are composed of alpha- and beta-subunits with an identical amino acid sequence but show different biological activities. To elucidate the molecular difference between these gonadotropins, the str

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c36f6bdf72d2e00fd2b77da4c51ac834
https://doi.org/10.1021/bi00251a012

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Evidence for two folding domains in glycoprotein hormone alpha-subunits

Autor: Darrell N. Ward, George R. Bousfield

Publikováno v: Endocrinology. 135:624-635

We reconstituted ovine (o) LH alpha from its amino- and carboxyl-terminal fragments obtained as follows. oLH alpha was nicked at Arg46-Ser47 with Arg-C protease. Nicked oLH alpha disulfide bonds were broken by sulfitolysis, and its N-terminal peptide

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b049c5ccdf017c6962744b3a95960601
https://doi.org/10.1210/endo.135.2.7518386

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Initial Characterization of Equine Inhibin1

Autor: Katherine H. Moore, Bonnie S. Dunbar, S George R. Bousfield, Darrell N. Ward

Publikováno v: Biology of Reproduction. 51:63-71

Inhibin has been characterized from a number of mammals; however, it has not been extensively studied in horses. Western blot analysis was used to examine the size heterogeneity of equine inhibin a- and [S-subunits. The distribution of equine inhibin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f66380efd4b7d130f6dca2d36957b554
https://doi.org/10.1095/biolreprod51.1.63

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Chemical cross-linking of porcine luteinizing hormone: location of the cross-link and consequences for stability and biological activity

Autor: S van Dijk, Darrell N. Ward

Publikováno v: Endocrinology. 132:534-538

To study the interaction between the subunits of LH and determine which amino acid residues are involved in this interaction, porcine and ovine LH (pLH and oLH) were cross-linked with 0.02 M 1-ethyl-(3-(3-dimethylaminopropyl)carbodiimide to generate

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d538693b7ee704562095d8ca2f815c67
https://doi.org/10.1210/endo.132.2.8425474

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Heterogeneity in porcine pituitary luteinizing hormone: amino acid and carbohydrate analysis

Autor: Darrell N. Ward, S van Dijk

Publikováno v: Endocrinology. 130:1966-1974

Pituitary LH from porcine pituitary glands was purified by a buffered ethanol extraction procedure, ion exchange on DEAE- and carboxymethyl-cellulose, and molecular exclusion on Sephacryl S-200. Purity was assessed by amino acid composition, N-termin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c57a686b02c69214a5e66d18177e22bb
https://doi.org/10.1210/endo.130.4.1547724

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Expression of biologically active heterodimeric bovine follicle-stimulating hormone in milk of transgenic mice

Autor: Jeffrey M. Rosen, Jerry J. Reeves, Joseph W. Anderson, Keiji Nishimori, Darrell N. Ward, David D. Deavila, Norman M. Greenberg, Aaron J. W. Hsueh

Publikováno v: Proceedings of the National Academy of Sciences. 88:8327-8331

Follicle-stimulating hormone (FSH; follitropin) is a pituitary glycoprotein composed of two post-translationally modified subunits, which must properly assemble to be biologically active. FSH has been difficult to purify and to obtain in quantities s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0954e154b1ec88126e330f39360a8053
https://doi.org/10.1073/pnas.88.19.8327

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GonadotropinsChemistry and Biosynthesis

Autor: George R. Bousfield, Li Jia, Darrell N. Ward

Publikováno v: Knobil and Neill's Physiology of Reproduction (Third Edition)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::928651c205738b68b77ca152ad7867c0
https://doi.org/10.1016/b978-012515400-0/50035-x

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Circular dichroic spectroscopy of Arg46-nicked ovine lutropin alpha and derived fragments

Autor: David Puett, George R. Bousfield, Kou-Cheng Peng, Darrell N. Ward

Publikováno v: Journal of protein chemistry. 15(6)

The alpha subunit of ovine lutropin can be nicked with the endoproteinase Arg-C to give a single cleavage of the Arg46-Ser47 peptide bond. Following reduction by sulfitolysis, the N-terminal (residues 1-46) and C-terminal (residues 47-96) fragments c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c313d699db9c3b9170939b047f56dca5
https://pubmed.ncbi.nlm.nih.gov/8895101

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