Zobrazeno 1 - 10
of 37
pro vyhledávání: '"Darrell Cockburn"'
Autor:
Darrell Cockburn, Casper Wilkens, Adiphol Dilokpimol, Hiroyuki Nakai, Anna Lewińska, Maher Abou Hachem, Birte Svensson
Publikováno v:
PLoS ONE, Vol 11, Iss 8, p e0160112 (2016)
Carbohydrate active enzymes often contain auxiliary binding sites located either on independent domains termed carbohydrate binding modules (CBMs) or as so-called surface binding sites (SBSs) on the catalytic module at a certain distance from the act
Externí odkaz:
https://doaj.org/article/1bcd987e28e34267b6e0166ee6f8ab01
Autor:
Darrell Cockburn, Nicole Koropatkin
Publikováno v:
Bio-Protocol, Vol 5, Iss 20 (2015)
Thin layer chromatography (TLC) is a useful technique for detecting the presence of monosaccharides through to oligosaccharides, though it needs to be optimized for the specific sugars that are analyzed. Here we present a method for visualizing the r
Externí odkaz:
https://doaj.org/article/2343f1eb3180412b9079636e09752a67
Autor:
Darrell Cockburn, Peter DeMartino
Publikováno v:
Current Opinion in Biotechnology. 61:66-71
Resistant starch has received a lot of attention for its potential to exert a healthy impact on the gut and certain members of its resident microbiota, particularly through enhanced butyrate production. However, resistant starch is a broad category t
Publikováno v:
Amylase. 4:24-44
Bacteria in the human gut including Ruminococcus bromii and Eubacterium rectale encode starch-active enzymes that dictate how these bacteria interact with starch to initiate a metabolic cascade that leads to increased butyrate. Here, we determined th
Publikováno v:
Beverages
Volume 7
Issue 3
Beverages, Vol 7, Iss 53, p 53 (2021)
Volume 7
Issue 3
Beverages, Vol 7, Iss 53, p 53 (2021)
The mashing step underpins the brewing process, during which the endogenous amylolytic enzymes in the malt, chiefly β-amylase, α-amylase, and limit dextrinase, act concurrently to rapidly hydrolyze malt starch to fermentable sugars. With barley mal
Autor:
Darrell Cockburn, June Teichmann
Publikováno v:
Frontiers in Microbiology
Frontiers in Microbiology, Vol 12 (2021)
Frontiers in Microbiology, Vol 12 (2021)
One of the primary benefits associated with dietary resistant starch (RS) is the production of butyrate by the gut microbiome during fermentation of this fiber in the large intestine. The ability to degrade RS is a relatively rare trait among microbe
Autor:
Darrell Cockburn, Nicole M. Koropatkin, Ryan Kibler, Haley A. Brown, Rebecca M. Duvall, Sarah Moraïs, Edward A. Bayer
Publikováno v:
Journal of structural biology. 213(3)
Pullulanases are glycoside hydrolase family 13 (GH13) enzymes that target α1,6 glucosidic linkages within starch and aid in the degradation of the α1,4- and α1,6- linked glucans pullulan, glycogen and amylopectin. The human gut bacterium Ruminococ
Publikováno v:
Protein Science. 27:1093-1101
Bacteria that reside in the mammalian intestinal tract efficiently hydrolyze dietary carbohydrates, including starch, that escape digestion in the small intestine. Starch is an abundant dietary carbohydrate comprised of α1,4 and α1,6 linked glucose
Autor:
Sarah Moraïs, Itzhak Mizrahi, Darrell Cockburn, Nicole M. Koropatkin, Eric C. Martens, Edward A. Bayer, Yonit Ben-David, Sylvia H. Duncan, Harry J. Flint
Publikováno v:
Environmental Microbiology. 18:5112-5122
Ruminococcus champanellensis is a keystone species in the human gut that produces an intricate cellulosome system of various architectures. A variety of cellulosomal enzymes have been identified, which exhibit a range of hydrolytic activities on lign
Autor:
Darrell Cockburn, Nicole M. Koropatkin
Publikováno v:
Journal of Molecular Biology. 428:3230-3252
Carbohydrates comprise a large fraction of the typical diet, yet humans are only able to directly process some types of starch and simple sugars. The remainder transits the large intestine where it becomes food for the commensal bacterial community.