Zobrazeno 1 - 10
of 24
pro vyhledávání: '"Dario Meluzzi"'
Autor:
Kyle Ford, Brenton P. Munson, Samson H. Fong, Rebecca Panwala, Wai Keung Chu, Joseph Rainaldi, Nongluk Plongthongkum, Vinayagam Arunachalam, Jarek Kostrowicki, Dario Meluzzi, Jason F. Kreisberg, Kristen Jensen-Pergakes, Todd VanArsdale, Thomas Paul, Pablo Tamayo, Kun Zhang, Jadwiga Bienkowska, Prashant Mali, Trey Ideker
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-20 (2023)
Abstract Cell-cycle control is accomplished by cyclin-dependent kinases (CDKs), motivating extensive research into CDK targeting small-molecule drugs as cancer therapeutics. Here we use combinatorial CRISPR/Cas9 perturbations to uncover an extensive
Externí odkaz:
https://doaj.org/article/fcd5b895626948aba19a440f77fb4bf1
Publikováno v:
eLife, Vol 11 (2022)
Adenosine deaminases acting on RNA (ADARs) can be repurposed to enable programmable RNA editing, however their exogenous delivery leads to transcriptome-wide off-targeting, and additionally, enzymatic activity on certain RNA motifs, especially those
Externí odkaz:
https://doaj.org/article/73940bf37fb4455a9643d0af3f0b4fdc
Autor:
Amir Gamliel, Dario Meluzzi, Soohwan Oh, Nan Jiang, Eugin Destici, Michael G. Rosenfeld, Sreejith J. Nair
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America, vol 119, iss 32
The eukaryotic genome is partitioned into distinct topological domains separated by boundary elements. Emerging data support the concept that several well-established nuclear compartments are ribonucleoprotein condensates assembled through the physic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0dfad534ddc0ce87f189297eb4918508
https://pubmed.ncbi.nlm.nih.gov/35914133
https://pubmed.ncbi.nlm.nih.gov/35914133
Autor:
Dhruva Katrekar, James Yen, Yichen Xiang, Anushka Saha, Dario Meluzzi, Yiannis Savva, Prashant Mali
Publikováno v:
Nature biotechnology, vol 40, iss 6
Recruiting endogenous adenosine deaminases using exogenous guide RNAs to edit cellular RNAs is a promising therapeutic strategy, but editing efficiency and durability remain low using current guide RNA designs. In this study, we engineered circular A
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15e9dbb4347070021f13af6a7fb1183b
https://escholarship.org/uc/item/5zq150jc
https://escholarship.org/uc/item/5zq150jc
Autor:
Qi Ma, Kenneth A. Ohgi, Tom Suter, Soohwan Oh, Priya R. Banerjee, Lu Yang, Jie Zhang, Meyer J. Friedman, Amir Gamliel, Yiren Hu, Aneel K. Aggarwal, Feng Yang, Dario Meluzzi, Susan Wang, Ranveer Singh Jayani, Yuliang Tan, Ibraheem Alshareedah, Sreejith J Nair, Michael G. Rosenfeld
Publikováno v:
Nature structural & molecular biology, vol 26, iss 3
A crucial feature of differentiated cells is the rapid activation of enhancer-driven transcriptional programs in response to signals. The potential contributions of physicochemical properties of enhancer assembly in signaling events remain poorly und
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60dbddbe4124804483d8e658b8c8b6ba
https://doi.org/10.1038/s41594-019-0190-5
https://doi.org/10.1038/s41594-019-0190-5
Autor:
Genghao Chen, Prashant Mali, Ashwin Ganesh, Yu Ru V. Shih, Dario Meluzzi, Atharv Worlikar, Shyni Varghese, Dhruva Katrekar
Publikováno v:
Nature methods, vol 16, iss 3
Nature methods
Nature methods
We present in vivo sequence-specific RNA base editing via adenosine deaminases acting on RNA (ADAR) enzymes with associated ADAR guide RNAs (adRNAs). To achieve this, we systematically engineered adRNAs to harness ADARs, and comprehensively evaluated
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c095da4f8ec9773531f525df8d6ecefd
https://doi.org/10.1038/s41592-019-0323-0
https://doi.org/10.1038/s41592-019-0323-0
Autor:
Prashant Mali, Yichen Xiang, Yiannis A. Savva, Dario Meluzzi, James Yen, Dhruva Katrekar, Anushka Saha
Akin to short-hairpin RNAs and antisense oligonucleotides which efficaciously recruit endogenous cellular machinery such as Argonaute and RNase H to enable targeted RNA knockdown, simple long antisense guide RNAs (1) can recruit endogenous adenosine
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::53efd543fb08ce93aa7b5971010ac6fb
https://doi.org/10.1101/2021.01.12.426286
https://doi.org/10.1101/2021.01.12.426286
Autor:
Gaurav Arya, Dario Meluzzi
Publikováno v:
Methods
Chromosome conformation capture (3C) and its variants are powerful experimental techniques for probing intra- and inter-chromosomal interactions within cell nuclei at high resolution and in a high-throughput, quantitative manner. The contact maps der
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d35881fc788d4d5d67b1de57d2ad54e7
https://pubmed.ncbi.nlm.nih.gov/31470090
https://pubmed.ncbi.nlm.nih.gov/31470090
Publikováno v:
Annual review of biophysics. 39
Knots appear in a wide variety of biophysical systems, ranging from biopolymers, such as DNA and proteins, to macroscopic objects, such as umbilical cords and catheters. Although significant advancements have been made in the mathematical theory of k
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9555cfe5602a0d342609b4c10f703460
https://pubmed.ncbi.nlm.nih.gov/20192771
https://pubmed.ncbi.nlm.nih.gov/20192771