Zobrazeno 1 - 10
of 33
pro vyhledávání: '"Dario Alessi"'
Autor:
Maurizio Ballico, Dario Alessi, Eleonora Aneggi, Marta Busato, Daniele Zuccaccia, Lorenzo Allegri, Giuseppe Damante, Christian Jandl, Walter Baratta
Publikováno v:
Molecules, Vol 29, Iss 9, p 2146 (2024)
The cyclometalated terpyridine complexes [Ru(η2-OAc)(NC-tpy)(PP)] (PP = dppb 1, (R,R)-Skewphos 4, (S,S)-Skewphos 5) are easily obtained from the acetate derivatives [Ru(η2-OAc)2(PP)] (PP = dppb, (R,R)-Skewphos 2, (S,S)-Skewphos 3) and tpy in methan
Externí odkaz:
https://doaj.org/article/9d26ac985d06439882c15b3c543df0d6
Autor:
Rotimi Fasimoye, Dario Alessi
Raw data files used for the manuscript "Golgi-IP, a novel tool for multimodal analysis of Golgi molecular content". The Word document (GolgiTAG paper_ file description Zenodo.docx) contains a description of each file.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47a860dce19fbd879dc859167151ca76
Preparation and imaging of enriched Golgi from GolgiTAG-IP using Transmission Electron Microscopy v1
Autor:
Dario Alessi, Rotimi Fasimoye
Transmission electron microscopy (TEM) is a tool used to image, in good resolution, the structure of organelles within the cell. Available protocols are designed to image structures within fixed intact cells. Here, we described a protocol where Golgi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::28313bf62a234de78451c3d6eb25261f
https://doi.org/10.17504/protocols.io.x54v9y9nqg3e/v1
https://doi.org/10.17504/protocols.io.x54v9y9nqg3e/v1
Purification of intact organelles by previously described methods (dx.doi.org/10.17504/protocols.io.bybjpskn; dx.doi.org/10.17504/protocols.io.6qpvrdjrogmk/v1) allows to profile the organelle proteome using quantitative mass spectrometry. Here we pro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ecfc06d0a1af2430fa0cbc1b5d94c402
https://doi.org/10.17504/protocols.io.kxygxzrokv8j/v1
https://doi.org/10.17504/protocols.io.kxygxzrokv8j/v1
Autor:
Dario Alessi, Rotimi Fasimoye
Analysis of the expression of organelle-specific markers is essential to verify the efficiency of any Golgi immunoprecipitation (IP) protocol. Here, we describe our immunoblotting method to assess efficient enrichment of Golgi proteins in Golgi immun
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::07bbb46b3650f8c28a5a33d22dee80bb
https://doi.org/10.17504/protocols.io.bp2l61oxdvqe/v1
https://doi.org/10.17504/protocols.io.bp2l61oxdvqe/v1
Autor:
Dario Alessi, Rotimi Fasimoye
Immunofluorescent (IF) microscopy is a powerful tool used in cellular and molecular biology to monitor the subcellular localisation of proteins. By combining the advantages of immunostaining and confocal light microscope, IF microscopy can be used to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e235a862dff2b93f8aa8674eff2644e4
https://doi.org/10.17504/protocols.io.q26g74qpkgwz/v1
https://doi.org/10.17504/protocols.io.q26g74qpkgwz/v1
Raw data files used for the manuscript "Impact of 100LRRK2 variants linked to Parkinson’s Disease on kinase activity and microtubule binding". This is a revised versionof the manuscript"Impact of 98 LRRK2 variants linked to Parkinson's Disease on k
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d8a65469d642312be10639e3b9ce14d
Autor:
Xingui Liu, Alexia Kalogeropulou, Sofia Domingos, Nikolai Makukhin, Raja Nirujogi, Francois Singh, Natalia Shpiro, Anton Saalfrank, Esther Sammler, Ian Ganley, Rui Moreira, Dario Alessi, Alessio Ciulli
Leucine Rich Repeat Kinase 2 (LRRK2) is one of the most promising targets for Parkinson’s Disease. LRRK2 targeting strategies have primarily focused on Type 1 kinase inhibitors, which however have limitations as the inhibited protein can interfere
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::213a6be7c5453a5c33432f09907f7fdf
https://doi.org/10.26434/chemrxiv-2022-4gzm0-v2
https://doi.org/10.26434/chemrxiv-2022-4gzm0-v2
Autor:
Suzanne Pfeffer, Dario Alessi
We present here a protocol for fractionating crude cellular extracts to prepare membrane and cytosol-enriched fractions and a nuclei-containing insoluble fraction from cultured cells. We deploy this protocol for determining the membrane versus cytoso
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0de23a9edb8366f68717af460bf2b948
https://doi.org/10.17504/protocols.io.yxmvmnb99g3p/v1
https://doi.org/10.17504/protocols.io.yxmvmnb99g3p/v1
We describe a non-radioactive, mass spectrometry-based assay that we deploy for identifying novel PKC-regulated sites on LRRK1 that are responsible for activation of its kinase activity.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8e6e487e2cd899084c473111d0bbbb40
https://doi.org/10.17504/protocols.io.261gen89dg47/v1
https://doi.org/10.17504/protocols.io.261gen89dg47/v1