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pro vyhledávání: '"Danylo Vyhovskyi"'
Autor:
Olga Musharova, Danylo Vyhovskyi, Sofia Medvedeva, Jelena Guzina, Yulia Zhitnyuk, Marko Djordjevic, Konstantin Severinov, Ekaterina Savitskaya
Publikováno v:
mBio, Vol 9, Iss 6 (2018)
ABSTRACT CRISPR DNA arrays of unique spacers separated by identical repeats ensure prokaryotic immunity through specific targeting of foreign nucleic acids complementary to spacers. New spacers are acquired into a CRISPR array in a process of CRISPR
Externí odkaz:
https://doaj.org/article/7aa6c4b730ee47aeb0dc8159091991c9
Autor:
William Rostain, Theophile Grebert, Danylo Vyhovskyi, Paula Thiel Pizarro, Gatwa Tshinsele-Van Bellingen, Lun Cui, David Bikard
Publikováno v:
Nucleic Acids Research
Nucleic Acids Research, 2023, 51 (7), pp.3485-3496. ⟨10.1093/nar/gkad170⟩
Nucleic Acids Research, 2023, 51 (7), pp.3485-3496. ⟨10.1093/nar/gkad170⟩
Genetic tools derived from the Cas9 RNA-guided nuclease are providing essential capabilities to study and engineer bacteria. While the importance of off-target effects was noted early in Cas9’s application to mammalian cells, off-target cleavage by
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d5a2a90b6a567fdcddb4f2416aa3ddf
https://hal-pasteur.archives-ouvertes.fr/pasteur-04130634/document
https://hal-pasteur.archives-ouvertes.fr/pasteur-04130634/document
Publikováno v:
Biochemical Society Transactions
Prokaryotic adaptive immunity is built when short DNA fragments called spacers are acquired into CRISPR (clustered regularly interspaced short palindromic repeats) arrays. CRISPR adaptation is a multistep process which comprises selection, generation
Autor:
Danylo Vyhovskyi, Sofia Medvedeva, Konstantin Severinov, Ekaterina Savitskaya, Olga Musharova, Yulia Zhitnyuk, Jelena Guzina, Marko Djordjevic
Publikováno v:
mBio, Vol 9, Iss 6 (2018)
mBio, Vol 9, Iss 6, p e02169-18 (2018)
mBio
mBio, Vol 9, Iss 6, p e02169-18 (2018)
mBio
Adaptive immunity of prokaryotes depends on acquisition of foreign DNA fragments into CRISPR arrays as spacers followed by destruction of foreign DNA by CRISPR interference machinery. Different fragments are acquired into CRISPR arrays with widely di