Structural basis for DNA damage-induced phosphoregulation of MDM2 RING domain

Autor: Helge M. Magnussen, Syed F. Ahmed, Gary. J. Sibbet, Ventzislava A. Hristova, Koji Nomura, Andreas K. Hock, Lewis J. Archibald, Andrew G. Jamieson, David Fushman, Karen H. Vousden, Allan M. Weissman, Danny T. Huang

Publikováno v: Nature Communications, Vol 11, Iss 1, Pp 1-15 (2020)

p53 is an important tumor suppressor protein which is regulated by the E3 ubiquitin ligase MDM2. Here the authors reveal that DNA damage-induced Ser429 phosphorylation of MDM2 serve to boost the activity of MDM2 homodimer by stabilizing the active E2

Externí odkaz: https://doaj.org/article/80e8bf383dda460bb88d16b6b1cd6555

Hepatic glutamine synthetase controls N5-methylglutamine in homeostasis and cancer

Autor: Victor H. Villar, Maria Francesca Allega, Ruhi Deshmukh, Tobias Ackermann, Mark A. Nakasone, Johan Vande Voorde, Thomas M. Drake, Janina Oetjen, Algernon Bloom, Colin Nixon, Miryam Müller, Stephanie May, Ee Hong Tan, Lars Vereecke, Maude Jans, Gillian Blancke, Daniel J. Murphy, Danny T. Huang, David Y. Lewis, Thomas G. Bird, Owen J. Sansom, Karen Blyth, David Sumpton, Saverio Tardito

Publikováno v: NATURE CHEMICAL BIOLOGY
Villar, V H, Allega, M F, Deshmukh, R, Ackermann, T, Nakasone, M A, Voorde, J V, Drake, T, Oetjen, J, Bloom, A, Nixon, C, Vereecke, L, Jans, M, Blancke, G, Bird, T G, Lewis, D, Sansom, O J, Blyth, K, Sumpton, D & Tardito, S 2022, ' Hepatic glutamine synthetase controls N5-methylglutamine in homeostasis and cancer ', Nature Chemical Biology . https://doi.org/10.1038/s41589-022-01154-9

Glutamine synthetase (GS) activity is conserved from prokaryotes to humans, where the ATP-dependent production of glutamine from glutamate and ammonia is essential for neurotransmission and ammonia detoxification. Here, we show that mammalian GS uses

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d644a6f88baef6ab2fcb6f68543200e9
https://doi.org/10.1038/s41589-022-01154-9

Author Reply to Peer Reviews of PTEN deficiency exposes a requirement for an ARF GTPase module in integrin-dependent invasion in ovarian cancer

Autor: Konstantina Nikolatou, Emma Sandilands, Alvaro Roman-Fernandez, Erin M. Cumming, Eva C. Freckmann, Sergio Lilla, Lori Buetow, Lynn McGarry, Matthew Neilson, Robin Shaw, David Strachan, Crispin Miller, Danny T Huang, Ian McNeish, James C. Norman, Sara Zanivan, David M Bryant

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3558da1a05c3ad0c5ac2fe3772e9e225
https://doi.org/10.15252/rc.2023374128

E3 ligase-inactivation rewires CBL interactome to elicit oncogenesis by hijacking RTK–CBL–CIN85 axis

Autor: Danny T. Huang, David Sumpton, Sergio Lilla, Lori Buetow, Syed Feroj Ahmed, William C. Clark, Gary J. Sibbet, Ann Hedley, Sara Zanivan, Mads Gabrielsen

Publikováno v: Oncogene

Casitas B-lineage lymphoma (CBL) is a ubiquitin ligase (E3) that becomes activated upon Tyr371-phosphorylation and targets receptor protein tyrosine kinases for ubiquitin-mediated degradation. Deregulation of CBL and its E3 activity is observed in my

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9005bbfbdbdd5e0fa9a704a9b8bd131e
https://doi.org/10.1038/s41388-021-01684-x

Structure of UBE2K-Ub/E3/polyUb reveals mechanisms of K48-linked Ub chain extension

Autor: Mark A, Nakasone, Karolina A, Majorek, Mads, Gabrielsen, Gary J, Sibbet, Brian O, Smith, Danny T, Huang

Publikováno v: Nature chemical biology. 18(4)

Ubiquitin (Ub) chain types govern distinct biological processes. K48-linked polyUb chains target substrates for proteasomal degradation, but the mechanism of Ub chain synthesis remains elusive due to the transient nature of Ub handover. Here, we pres

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::a423c870b00a52268d7ef3f5190bcd0f
https://pubmed.ncbi.nlm.nih.gov/35027744