CryoET reveals actin filaments within platelet microtubules

Autor: Chisato Tsuji, Marston Bradshaw, Megan F. Allen, Molly L. Jackson, Judith Mantell, Ufuk Borucu, Alastair W. Poole, Paul Verkade, Ingeborg Hers, Danielle M. Paul, Mark P. Dodding

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-8 (2024)

Abstract Crosstalk between the actin and microtubule cytoskeletons is important for many cellular processes. Recent studies have shown that microtubules and F-actin can assemble to form a composite structure where F-actin occupies the microtubule lum

Externí odkaz: https://doaj.org/article/9f1e9987006d4254bcc55c15f45b131c

In situ cryo-electron tomography reveals filamentous actin within the microtubule lumen

Autor: Danielle M. Paul, Jennifer Coombs, Katherine J Surridge, Ufuk Borucu, John M. Squire, Mark P. Dodding, Paul Verkade, Judith Mantell

Publikováno v: Paul, D M, Mantell, J M, Borucu, U, Coombs, J, Surridge, K J, Squire, J M, Verkade, P & Dodding, M P 2020, ' In situ cryo-electron tomography reveals filamentous actin within the microtubule lumen ', Journal of Cell Biology, vol. 219, no. 9, e201911154 . https://doi.org/10.1083/jcb.201911154
The Journal of Cell Biology

Paul et al. use cryo-ET to show that microtubules can contain actin filaments in their lumen. Two types of F-actin structure are distinguished, Class I and Class II, with slightly different helical symmetries and outer microtubule diameters. They cal

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c34746d0ffcc7be0b951aff58a935121
https://hdl.handle.net/1983/f4f23cfd-eea3-47a0-a22c-390c3e979adf

After the revolution: how is Cryo-EM contributing to muscle research?

Autor: Marston Bradshaw, Danielle M. Paul

Publikováno v: Journal of Muscle Research and Cell Motility
Bradshaw, M & Paul, D M 2019, ' After the revolution : how is Cryo-EM contributing to muscle research? ', Journal of Muscle Research and Cell Motility, vol. 40, no. 2, pp. 93-98 . https://doi.org/10.1007/s10974-019-09537-7

The technique of electron microscopy (EM) has been fundamental to muscle research since the days of Huxley and Hanson. Direct observation of how proteins in the sarcomere are arranged and visualising the changes that occur upon activation have greatl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06af9b25588514a35d8deef729de69b4
https://pubmed.ncbi.nlm.nih.gov/31302812

Myosin and Actin Filaments in Muscle: Structures and Interactions

Autor: Danielle M. Paul, Edward P. Morris, John M. Squire

Publikováno v: Subcellular Biochemistry ISBN: 9783319496726

In the last decade, improvements in electron microscopy and image processing have permitted significantly higher resolutions to be achieved (sometimes

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6ffc2307b9d40f026cc61813c7cd44f9
https://doi.org/10.1007/978-3-319-49674-0_11

Structure and orientation of troponin in the thin filament

Autor: Robert W. Kensler, Edward P. Morris, Danielle M. Paul, John M. Squire

Publikováno v: The Journal of Biological Chemistry

The troponin complex on the thin filament plays a crucial role in the regulation of muscle contraction. However, the precise location of troponin relative to actin and tropomyosin remains uncertain. We have developed a method of reconstructing thin f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26c9150e57e7446515d5226fe84f7edf
https://pubmed.ncbi.nlm.nih.gov/19321455