Rate-limiting transport of positively charged arginine residues through the Sec-machinery is integral to the mechanism of protein secretion

Autor: William J Allen, Robin A Corey, Daniel W Watkins, A Sofia F Oliveira, Kiel Hards, Gregory M Cook, Ian Collinson

Publikováno v: eLife, Vol 11 (2022)

Transport of proteins across and into membranes is a fundamental biological process with the vast majority being conducted by the ubiquitous Sec machinery. In bacteria, this is usually achieved when the SecY-complex engages the cytosolic ATPase SecA

Externí odkaz: https://doaj.org/article/1b494683a74344b6a9b7e11b4db47ad9

Association of Fluorescent Protein Pairs and Its Significant Impact on Fluorescence and Energy Transfer

Autor: Jacob R. Pope, Rachel L. Johnson, W. David Jamieson, Harley L. Worthy, Senthilkumar Kailasam, Rochelle D. Ahmed, Ismail Taban, Husam Sabah Auhim, Daniel W. Watkins, Pierre J. Rizkallah, Oliver K. Castell, D. Dafydd Jones

Publikováno v: Advanced Science, Vol 8, Iss 1, Pp n/a-n/a (2021)

Abstract Fluorescent proteins (FPs) are commonly used in pairs to monitor dynamic biomolecular events through changes in proximity via distance dependent processes such as Förster resonance energy transfer (FRET). The impact of FP association is ass

Externí odkaz: https://doaj.org/article/7938b9fd96124ed0a636bbaf8e40a461

A bacterial secretosome for regulated envelope biogenesis and quality control?

Autor: Daniel W. Watkins, Sophie L. Williams, Ian Collinson

Publikováno v: Watkins, D W, Williams, S L & Collinson, I 2022, ' A bacterial secretosome for regulated envelope biogenesis and quality control? ', Microbiology (Reading, England), vol. 168, no. 10, 001255 . https://doi.org/10.1099/mic.0.001255

The Gram-negative bacterial envelope is the first line of defence against environmental stress and antibiotics. Therefore, its biogenesis is of considerable fundamental interest, as well as a challenge to address the growing problem of antimicrobial

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a30d0a0c5f801cef7d4a52c1ccdbf4c1
https://hdl.handle.net/1983/12f716fd-2e45-49cd-973c-a77ee69ca298

Refined measurement of SecA-driven protein secretion reveals that translocation is indirectly coupled to ATP turnover

Autor: Mark S. Dillingham, Ian Collinson, William J. Allen, Daniel W. Watkins

Publikováno v: Allen, W J, Watkins, D W, Dillingham, M S & Collinson, I 2020, ' Refined measurement of SecA-driven protein secretion reveals that translocation is indirectly coupled to ATP turnover ', Proceedings of the National Academy of Sciences of the United States of America . https://doi.org/10.1073/pnas.2010906117
Proc Natl Acad Sci U S A

The universally conserved Sec system is the primary method cells utilise totransport proteins across membranes. Until recently, measuring the activity – aprerequisite for understanding how biological systems works – has been limited to discontinu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78b5e308ef298f34e91e891b548962ef
https://doi.org/10.1073/pnas.2010906117

Controlling Protein Nanocage Assembly with Hydrostatic Pressure

Autor: Adam W. Perriman, Ben M. Carter, Andrew J. Smith, Helmut Cölfen, Valeska P. Ting, Kristian Le Vay, T.-Y. Dora Tang, Robert P. Rambo, Daniel W. Watkins, J. L. Ross Anderson

Publikováno v: Le Vay, K, Carter, B M, Watkins, D W, Tang, T-Y D, Ting, V P, Cölfen, H, Rambo, R P, Smith, A J, Anderson, J L R & Perriman, A W 2020, ' Controlling protein nanocage assembly with hydrostatic pressure ', Journal of the American Chemical Society . https://doi.org/10.1021/jacs.0c07285

Controlling the assembly and disassembly of nanoscale protein cages for the capture and internalisation of protein or non-proteinaceous components is fundamentally important to a diverse range of bionanotechnological applications. Here, we study the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7927c49b2d17cf7dbaa601e31b4631f8