Rate-limiting transport of positively charged arginine residues through the Sec-machinery is integral to the mechanism of protein secretion

Autor: William J Allen, Robin A Corey, Daniel W Watkins, A Sofia F Oliveira, Kiel Hards, Gregory M Cook, Ian Collinson

Publikováno v: eLife, Vol 11 (2022)

Transport of proteins across and into membranes is a fundamental biological process with the vast majority being conducted by the ubiquitous Sec machinery. In bacteria, this is usually achieved when the SecY-complex engages the cytosolic ATPase SecA

Externí odkaz: https://doaj.org/article/1b494683a74344b6a9b7e11b4db47ad9

Inter-membrane association of the Sec and BAM translocons for bacterial outer-membrane biogenesis

Autor: Sara Alvira, Daniel W Watkins, Luca A Troman, William J Allen, James S Lorriman, Gianluca Degliesposti, Eli J Cohen, Morgan Beeby, Bertram Daum, Vicki AM Gold, J Mark Skehel, Ian Collinson

Publikováno v: eLife, Vol 9 (2020)

The outer-membrane of Gram-negative bacteria is critical for surface adhesion, pathogenicity, antibiotic resistance and survival. The major constituent – hydrophobic β-barrel Outer-Membrane Proteins (OMPs) – are first secreted across the inner-m

Externí odkaz: https://doaj.org/article/f24b1cd117cb40268b4bfbcedb0b00c8

A bacterial secretosome for regulated envelope biogenesis and quality control?

Autor: Daniel W. Watkins, Sophie L. Williams, Ian Collinson

Publikováno v: Watkins, D W, Williams, S L & Collinson, I 2022, ' A bacterial secretosome for regulated envelope biogenesis and quality control? ', Microbiology (Reading, England), vol. 168, no. 10, 001255 . https://doi.org/10.1099/mic.0.001255

The Gram-negative bacterial envelope is the first line of defence against environmental stress and antibiotics. Therefore, its biogenesis is of considerable fundamental interest, as well as a challenge to address the growing problem of antimicrobial

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a30d0a0c5f801cef7d4a52c1ccdbf4c1
https://hdl.handle.net/1983/12f716fd-2e45-49cd-973c-a77ee69ca298

Refined measurement of SecA-driven protein secretion reveals that translocation is indirectly coupled to ATP turnover

Autor: Mark S. Dillingham, Ian Collinson, William J. Allen, Daniel W. Watkins

Publikováno v: Allen, W J, Watkins, D W, Dillingham, M S & Collinson, I 2020, ' Refined measurement of SecA-driven protein secretion reveals that translocation is indirectly coupled to ATP turnover ', Proceedings of the National Academy of Sciences of the United States of America . https://doi.org/10.1073/pnas.2010906117
Proc Natl Acad Sci U S A

The universally conserved Sec system is the primary method cells utilise totransport proteins across membranes. Until recently, measuring the activity – aprerequisite for understanding how biological systems works – has been limited to discontinu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78b5e308ef298f34e91e891b548962ef
https://doi.org/10.1073/pnas.2010906117

Controlling Protein Nanocage Assembly with Hydrostatic Pressure

Autor: Adam W. Perriman, Ben M. Carter, Andrew J. Smith, Helmut Cölfen, Valeska P. Ting, Kristian Le Vay, T.-Y. Dora Tang, Robert P. Rambo, Daniel W. Watkins, J. L. Ross Anderson

Publikováno v: Le Vay, K, Carter, B M, Watkins, D W, Tang, T-Y D, Ting, V P, Cölfen, H, Rambo, R P, Smith, A J, Anderson, J L R & Perriman, A W 2020, ' Controlling protein nanocage assembly with hydrostatic pressure ', Journal of the American Chemical Society . https://doi.org/10.1021/jacs.0c07285

Controlling the assembly and disassembly of nanoscale protein cages for the capture and internalisation of protein or non-proteinaceous components is fundamentally important to a diverse range of bionanotechnological applications. Here, we study the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7927c49b2d17cf7dbaa601e31b4631f8