Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Daniel T Radoff"'
Publikováno v:
eLife, Vol 3 (2014)
The presynaptic protein complexin (CPX) is a critical regulator of synaptic vesicle fusion, but the mechanisms underlying its regulatory effects are not well understood. Its highly conserved central helix (CH) directly binds the ternary SNARE complex
Externí odkaz:
https://doaj.org/article/7e8d9e91972044a29909d61497abf177
Autor:
Daniel Kümmel, Shyam S. Krishnakumar, Feng Li, Karin M. Reinisch, Claudio G. Giraudo, Daniel T Radoff, James E. Rothman, Frederic Pincet
Publikováno v:
Nature structural & molecular biology
Summary Complexin prevents SNAREs from releasing neurotransmitters until an action potential arrives at the synapse. To understand the mechanism for this inhibition, we determined the structure of complexin bound to a mimetic of a pre-fusion SNAREpin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e7c23d3292a2a71fa321465ac7563ae
https://doi.org/10.1038/nsmb.2101
https://doi.org/10.1038/nsmb.2101
Autor:
Stephanie Wood Baguley, Feng Li, Karin M. Reinisch, Frederic Pincet, Daniel Kümmel, Lavan Khandan, Jeff Coleman, Shyam S. Krishnakumar, James E. Rothman, Daniel T Radoff, Claudio G. Giraudo
Publikováno v:
Nature Structural & Molecular Biology. 18:934-940
The crystal structure of complexin bound to a prefusion SNAREpin mimetic shows that the accessory helix extends away from the SNAREpin in an 'open' conformation, binding another SNAREpin and inhibiting its assembly, to clamp fusion. In contrast, the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8268e1498affea15852357ae2351f0be
https://doi.org/10.1038/nsmb.2103
https://doi.org/10.1038/nsmb.2103
Publikováno v:
eLife
eLife, Vol 3 (2014)
eLife, Vol 3 (2014)
The presynaptic protein complexin (CPX) is a critical regulator of synaptic vesicle fusion, but the mechanisms underlying its regulatory effects are not well understood. Its highly conserved central helix (CH) directly binds the ternary SNARE complex
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d321029cf1cdd823fb3a77cab785fad
http://europepmc.org/articles/PMC4270070
http://europepmc.org/articles/PMC4270070
Autor:
Sunny Jones, Karin M. Reinisch, Daniel T Radoff, James E. Rothman, Daniel Kümmel, Shyam S. Krishnakumar
Publikováno v:
Biophysical Journal. (11):2507-2516
Synaptotagmin triggers rapid exocytosis of neurotransmitters from synaptic vesicles in response to Calcium (Ca2+) ions. Here, we use a novel Nanodisc-based system, designed to be a soluble mimetic of the clamped synaptic vesicle-bilayer junction, com
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f963614d8daad49647ea3de6ec25e8b