Zobrazeno 1 - 10
of 44
pro vyhledávání: '"Daniel Ley"'
Autor:
Stefan Kol, Daniel Ley, Tune Wulff, Marianne Decker, Johnny Arnsdorf, Sanne Schoffelen, Anders Holmgaard Hansen, Tanja Lyholm Jensen, Jahir M. Gutierrez, Austin W. T. Chiang, Helen O. Masson, Bernhard O. Palsson, Bjørn G. Voldborg, Lasse Ebdrup Pedersen, Helene Faustrup Kildegaard, Gyun Min Lee, Nathan E. Lewis
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Host cell proteins can contaminate biotherapeutics and compromise and degrade their quality. Here the authors use modelling and CRISPR to delete secreted host proteins in CHO cells, leading to improved monoclonal antibody production and purity.
Externí odkaz:
https://doaj.org/article/fb48699f0dc94c81a1ca056457c7fde6
Autor:
Jahir M. Gutierrez, Amir Feizi, Shangzhong Li, Thomas B. Kallehauge, Hooman Hefzi, Lise M. Grav, Daniel Ley, Deniz Baycin Hizal, Michael J. Betenbaugh, Bjorn Voldborg, Helene Faustrup Kildegaard, Gyun Min Lee, Bernhard O. Palsson, Jens Nielsen, Nathan E. Lewis
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
The secretory pathway is used in the production of most biopharmaceuticals, but the associated biosynthetic costs are little understood. Here, the authors integrate the core secretory pathway into genome-scale metabolic models of human, mouse, and CH
Externí odkaz:
https://doaj.org/article/7b7b5cdd06f3404dbb201e1d925f5e77
Autor:
Anne Mathilde Davy, Helene Faustrup Kildegaard, Tune Wulff, Hooman Hefzi, Johnny Arnsdorf, Sara Pereira, Tae Kwang Ha, Lasse Ebdrup Pedersen, Mikael Rørdam Andersen, Daniel Ley
Publikováno v:
Ley, D, Pereira, S, Pedersen, L E, Arnsdorf, J, Hefzi, H, Davy, A M, Kwang Ha, T, Wulff, T, Kildegaard, H F & Andersen, M R 2019, ' Reprogramming AA catabolism in CHO cells with CRISPR/Cas9 genome editing improves cell growth and reduces byproduct secretion ', Metabolic Engineering, vol. 56, pp. 120-129 . https://doi.org/10.1016/j.ymben.2019.09.005
Chinese hamster ovary (CHO) cells are the preferred host for producing biopharmaceuticals. Amino acids are biologically important precursors for CHO metabolism; they serve as building blocks for proteogenesis, including synthesis of biomass and recom
Publikováno v:
BMC Public Health, Vol 24, Iss 1, Pp 1-14 (2024)
Abstract Background Physical activity has been proven to be beneficial for physical and psychological health as well as for academic achievement. However, especially university students are insufficiently physically active because of difficulties in
Externí odkaz:
https://doaj.org/article/c2b1d7a34f0a48ec9881624ae9dde45c
Autor:
Daniel Leykam
Publikováno v:
AAPPS Bulletin, Vol 34, Iss 1, Pp 1-8 (2024)
Abstract Flat bands arise in periodic media when symmetries or fine-tuning result in perfect wavepacket localisation. Flat band localisation is fragile and exhibits remarkably sharp sensitivity to perturbations including interactions and disorder, le
Externí odkaz:
https://doaj.org/article/588b870b5cf440ab8f741f8a567b5c9e
Autor:
Susann-Cathrin Olthof, Elisabeth Weiland, Thomas Benkert, Daniel Wessling, Daniel Leyhr, Saif Afat, Konstantin Nikolaou, Heike Preibsch
Publikováno v:
Diagnostics, Vol 14, Iss 16, p 1742 (2024)
The objective of this study was to evaluate a high-resolution deep-learning (DL)-based diffusion-weighted imaging (DWI) sequence for breast magnetic resonance imaging (MRI) in comparison to a standard DWI sequence (DWIStd) at 1.5 T. It is a prospecti
Externí odkaz:
https://doaj.org/article/40c3d0399bd346dcb82b3b9132f224fb
Autor:
Bernhard O. Palsson, Austin W. T. Chiang, Johnny Arnsdorf, Marianne Decker, Sanne Schoffelen, Tune Wulff, Lasse Ebdrup Pedersen, Helene Faustrup Kildegaard, Stefan Kol, Tanja Lyholm Jensen, Jahir M. Gutierrez, Gyun Min Lee, Bjørn G. Voldborg, Anders Holmgaard Hansen, Helen O. Masson, Daniel Ley, Nathan E. Lewis
Publikováno v:
Nature communications, vol 11, iss 1
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Kol, S, Ley, D, Wulff, T, Decker, M, Arnsdorf, J, Schoffelen, S, Hansen, A H, Jensen, T L, Gutierrez, J M, Chiang, A W T, Masson, H O, Palsson, B O, Voldborg, B G, Pedersen, L E, Kildegaard, H F, Min Lee, G & Lewis, N E 2020, ' Multiplex secretome engineering enhances recombinant protein production and purity ', Nature Communications, vol. 11, no. 1 . https://doi.org/10.1038/s41467-020-15866-w
Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Kol, S, Ley, D, Wulff, T, Decker, M, Arnsdorf, J, Schoffelen, S, Hansen, A H, Jensen, T L, Gutierrez, J M, Chiang, A W T, Masson, H O, Palsson, B O, Voldborg, B G, Pedersen, L E, Kildegaard, H F, Min Lee, G & Lewis, N E 2020, ' Multiplex secretome engineering enhances recombinant protein production and purity ', Nature Communications, vol. 11, no. 1 . https://doi.org/10.1038/s41467-020-15866-w
Nature Communications
Host cell proteins (HCPs) are process-related impurities generated during biotherapeutic protein production. HCPs can be problematic if they pose a significant metabolic demand, degrade product quality, or contaminate the final product. Here, we pres
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::39eafb13b29626fb0af0e6200e0258ea
https://escholarship.org/uc/item/6dr7w8j6
https://escholarship.org/uc/item/6dr7w8j6
Autor:
Carlos Daniel Ley Magana, Ida Schoultz, Carl Mårten Lindqvist, Åsa V. Keita, Ake Ost, Jonas Halfvarson
Publikováno v:
Journal of Crohn's & Colitis
Background and Aims The aetiology of Crohn’s disease is poorly understood. By investigating twin pairs discordant for Crohn’s disease, we aimed to assess whether the dysregulated barrier represents a cause or a consequence of inflammation and to
Autor:
Daniel Leykam, Dimitris G. Angelakis
Publikováno v:
Advances in Physics: X, Vol 8, Iss 1 (2023)
ABSTRACTTopological data analysis refers to approaches for systematically and reliably computing abstract ‘shapes’ of complex data sets. There are various applications of topological data analysis in life and data sciences, with growing interest
Externí odkaz:
https://doaj.org/article/9e9590a626f248a1978e662f13cf4a6b
Publikováno v:
Ley, D, Andersen, M R & Kildegaard, H F Feb. 22 2018, Method for reducing ammonium and lactate production in cho cells, Patent No. WO2018033542, IPC No. C12N15/52; C12P21/02 .
Technical University of Denmark Orbit
Technical University of Denmark Orbit
The present invention relates to modified producer cells for improved production of therapeutic proteins. Specifically, the inventors have found that removing genes involved in amino acid catabolism in Chinese Hamster Ovary (CHO) cells improves the c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::8bf3a27a789c2298d6d43cde7282fcfb
http://orbit.dtu.dk/ws/files/146143136/WO2018033542A1.pdf
http://orbit.dtu.dk/ws/files/146143136/WO2018033542A1.pdf